Basic Information | |
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Species | Carica papaya |
Cazyme ID | evm.model.supercontig_29.123 |
Family | GH32 |
Protein Properties | Length: 674 Molecular Weight: 74744.3 Isoelectric Point: 5.2337 |
Chromosome | Chromosome/Scaffold: 29 Start: 1160349 End: 1163240 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 134 | 449 | 0 |
HFQPEKNWMNGPMFYKGWYHFFYQYNPNGAVWGDIAWGHAVSKDLIRWFYLPLAMVPDQWYDINGVWSGSATILHDGQIVMLYTGSTNESVQVQNLAYPA DLSDPLLINWVKYSNNPVLVPPPGIHFKDFRDPTTAWLTLEGKWRITVGSKVNKTGISLVYDTNDFINFELLDSILHGVPGTGMWECVDFYPVSKTSNYG LDTSSYGPKVKHVVKASMDDTRNDHYAIGTYFEENGTWVPDFPEIDVGIGLRYDYGTFYASKTFYDQDKQRRVLWGWITESDSEAADVKKGWASVQSIPR TLVLDQQTGTNLLQWP |
Full Sequence |
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Protein Sequence Length: 674 Download |
MDGQKTFLPI SNTHVYTALP SGPHPVGPPA TDRRRPIKAF LVTSSGFLTL AFLVALIASY 60 NGDNLGNNNT VINNNDASLS YVSMEPAKLM GPVSRGSPAG VSEKSNRPLF LGLKAMPAFP 120 WSNDMLSWQR TSFHFQPEKN WMNGPMFYKG WYHFFYQYNP NGAVWGDIAW GHAVSKDLIR 180 WFYLPLAMVP DQWYDINGVW SGSATILHDG QIVMLYTGST NESVQVQNLA YPADLSDPLL 240 INWVKYSNNP VLVPPPGIHF KDFRDPTTAW LTLEGKWRIT VGSKVNKTGI SLVYDTNDFI 300 NFELLDSILH GVPGTGMWEC VDFYPVSKTS NYGLDTSSYG PKVKHVVKAS MDDTRNDHYA 360 IGTYFEENGT WVPDFPEIDV GIGLRYDYGT FYASKTFYDQ DKQRRVLWGW ITESDSEAAD 420 VKKGWASVQS IPRTLVLDQQ TGTNLLQWPV EEVEILRLSS KEFVDIKVGP GSVIPLDIGP 480 ATQLDIVAEF EVDKEALNGT AMTEVGFSCS AGGGAAQRGA LGPFGILVLA DESLSEQTPV 540 YFYVSKGSDG SFKTFFCTDT LRSSLATDVN KLVHWSFVPV LEGEKLSLRI LVDHSIVESF 600 AQGGRSAITA RVYPTKAIYG AAKIFLFNNA TDAKINASLK IWQMNSAFNR PYHDDDDDSD 660 LSSQPHIPNH YFS* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-49 | 130 | 614 | 505 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 1.0e-69 | 130 | 615 | 501 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 9.0e-91 | 140 | 452 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 2.0e-148 | 134 | 449 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 134 | 604 | 484 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAB82419.1 | 0 | 75 | 665 | 70 | 657 | acid invertase [Citrus unshiu] |
DDBJ | BAF34362.1 | 0 | 75 | 665 | 70 | 657 | beta-fructofuranosidase [Citrus sinensis] |
DDBJ | BAF35859.1 | 0 | 83 | 656 | 80 | 653 | soluble acid invertase [Pyrus pyrifolia var. culta] |
RefSeq | XP_002303519.1 | 0 | 7 | 655 | 7 | 660 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002532576.1 | 0 | 78 | 652 | 71 | 645 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 119 | 653 | 8 | 545 | A Chain A, Crystal Structure At 2.0 A Of The S-Component For Thiamin From An Ecf- Type Abc Transporter |
PDB | 3ugh_A | 0 | 119 | 653 | 8 | 545 | A Chain A, Crystal Structure At 2.0 A Of The S-Component For Thiamin From An Ecf- Type Abc Transporter |
PDB | 3ugg_B | 0 | 119 | 653 | 8 | 545 | A Chain A, Crystal Structure At 2.0 A Of The S-Component For Thiamin From An Ecf- Type Abc Transporter |
PDB | 3ugg_A | 0 | 119 | 653 | 8 | 545 | A Chain A, Crystal Structure At 2.0 A Of The S-Component For Thiamin From An Ecf- Type Abc Transporter |
PDB | 3ugf_B | 0 | 119 | 653 | 8 | 545 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
fructan biosynthesis | RXN-1801 | EC-2.4.1.100 | 2,1-fructan:2,1-fructan 1-fructosyltransferase |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |