| Basic Information | |
|---|---|
| Species | Chlamydomonas reinhardtii |
| Cazyme ID | g10103.t1 |
| Family | CBM20 |
| Protein Properties | Length: 791 Molecular Weight: 78159.9 Isoelectric Point: 8.5962 |
| Chromosome | Chromosome/Scaffold: 9 Start: 6504481 End: 6510318 |
| Description | Carbohydrate-binding-like fold |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM20 | 42 | 130 | 2.8e-25 |
| VAVSFRTQYKCDFGQALALVGDSVTLGSWKGTQAQRMKWTSGDQWTCTVALPAGSALECKYIVVDERTGREVRWQEGGNMLVTVPASVQ | |||
| Full Sequence |
|---|
| Protein Sequence Length: 791 Download |
| MQQQGFRSVQ RPAQLQARCR CQPYRPNVVI RASLSDSRPH MVAVSFRTQY KCDFGQALAL 60 VGDSVTLGSW KGTQAQRMKW TSGDQWTCTV ALPAGSALEC KYIVVDERTG REVRWQEGGN 120 MLVTVPASVQ SLPVEQYETR ISWCKQYSSF KAHPLTSVAA AAAGITAAAA AKSAAPVMAW 180 AAAKGASVAA AAAVGSSNSS SITATAVAAP PQQQQQLKAA GAGAAGAGGA GTAALGALPT 240 VSAAAAPDGL VATIAQAAAD AVLSGASLAG LPVATPPPGM AAGVGADASS GGAARFHLTP 300 ASEDEPLTLT LLPRSTSPPL KLSLSNNYSQ LMEMTGGGSS PGGGSSPSAS RSSSSANLAG 360 MAAASAATAA AATPATSAPA GSTSAASQIR SHPIEVRCSP WSEPLSQQQL QQRRASSTSS 420 SSSAAKQQQP QPAPKQPVAA LPAVSTASVS AWGAEYDMYA SLYDAPYMHT AFDLDAYALY 480 DTPSVPEPAS VASSVTARAR SPLAGGRAAS PAAAAGTAGT AAASIFGSPG GVKAASGCVD 540 PLESAVQAAR SSSPSKAAAA AAAPTAPPTS AGAAAAAAAP GSAPAYNSIF TDAPAASKQP 600 PSAAASAAAS ASATSSASST DAAAASSSYR STSRSGKAVA DWAGRTLFTL SSASASASIL 660 DSADSDSDEE DDEVSEHEAR VAHALTAPVT AADMAALLKN MGTALGHSVR LRHGGVEPAA 720 ADLLELDRQI ALQCSKLYRK RDNLLVGFVR QETARRQLAP AVAAAAAGGS GGSGAGGAGV 780 RAGSNSVTPM * 840 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| smart01065 | CBM_2 | 6.0e-14 | 42 | 127 | 90 | + Starch binding domain. | ||
| cd02853 | E_set_MTHase_like_N | 5.0e-14 | 42 | 128 | 87 | + N-terminal Early set domain associated with the catalytic domain of Maltooligosyl trehalose trehalohydrolase (also called Glycosyltrehalose trehalohydrolase) and similar proteins. E or "early" set domains are associated with the catalytic domain of Maltooligosyl trehalose trehalohydrolase (MTHase) and similar proteins at the N-terminal end. This subfamily also includes bacterial alpha amylases and 1,4-alpha-glucan branching enzymes which are highly similar to MTHase. Maltooligosyl trehalose synthase (MTSase) and MTHase work together to produce trehalose. MTSase is responsible for converting the alpha-1,4-glucosidic linkage to an alpha,alpha-1,1-glucosidic linkage at the reducing end of the maltooligosaccharide through an intramolecular transglucosylation reaction, while MTHase hydrolyzes the penultimate alpha-1,4 linkage of the reducing end, resulting in the release of trehalose. The N-terminal domain of MTHase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others. | ||
| cd05467 | CBM20 | 1.0e-17 | 44 | 128 | 88 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| pfam00686 | CBM_20 | 2.0e-19 | 41 | 131 | 94 | + Starch binding domain. | ||
| cd02861 | E_set_pullulanase_like | 2.0e-20 | 44 | 125 | 82 | + Early set domain associated with the catalytic domain of pullulanase-like proteins. E or "early" set domains are associated with the catalytic domain of pullulanase at either the N-terminal or C-terminal end, and in a few instances at both ends. Pullulanase (also called dextrinase or alpha-dextrin endo-1,6-alpha glucosidase) is an enzyme with action similar to that of isoamylase; it cleaves 1,6-alpha-glucosidic linkages in pullulan, amylopectin, and glycogen, and in alpha-and beta-amylase limit-dextrins of amylopectin and glycogen. The E set domain of pullulanase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase. This domain is also a member of the CBM48 (Carbohydrate Binding Module 48) family whose members include maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, isoamylase, and the beta subunit of AMP-activated protein kinase. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | XP_001696233.1 | 0.000000000002 | 19 | 104 | 12 | 90 | hypothetical protein CHLREDRAFT_182288 [Chlamydomonas reinhardtii] |
| RefSeq | XP_001697682.1 | 0 | 1 | 163 | 1 | 163 | hypothetical protein CHLREDRAFT_192837 [Chlamydomonas reinhardtii] |
| RefSeq | XP_001697682.1 | 0.00004 | 254 | 283 | 155 | 184 | hypothetical protein CHLREDRAFT_192837 [Chlamydomonas reinhardtii] |
| RefSeq | XP_001697682.1 | 2.8026e-45 | 665 | 745 | 208 | 288 | hypothetical protein CHLREDRAFT_192837 [Chlamydomonas reinhardtii] |
| RefSeq | XP_001770618.1 | 0.000000000002 | 35 | 135 | 104 | 207 | predicted protein [Physcomitrella patens subsp. patens] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1kum_A | 0.000004 | 37 | 126 | 2 | 93 | A Chain A, Wif Domain-Egf-Like Domain 1 Of Human Wnt Inhibitory Factor 1 In Complex With 1,2-Dipalmitoylphosphatidylcholine |
| PDB | 1kul_A | 0.000004 | 37 | 126 | 2 | 93 | A Chain A, Wif Domain-Egf-Like Domain 1 Of Human Wnt Inhibitory Factor 1 In Complex With 1,2-Dipalmitoylphosphatidylcholine |
| PDB | 1acz_A | 0.000004 | 37 | 126 | 2 | 93 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, 5 Structures |
| PDB | 1ac0_A | 0.000004 | 37 | 126 | 2 | 93 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| PDB | 2vn7_A | 0.001 | 23 | 127 | 485 | 584 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| FC374099 | 105 | 35 | 135 | 0.0000000000006 |
| DC911534 | 105 | 35 | 135 | 0.000000000002 |
| BY992120 | 98 | 35 | 132 | 0.000000000004 |
| BI529517 | 88 | 17 | 104 | 0.000000000006 |
| BQ820503 | 88 | 17 | 104 | 0.000000000006 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
