| Basic Information | |
|---|---|
| Species | Mimulus guttatus |
| Cazyme ID | mgv1a001031m |
| Family | GT20 |
| Protein Properties | Length: 909 Molecular Weight: 102127 Isoelectric Point: 6.3761 |
| Chromosome | Chromosome/Scaffold: 174 Start: 440181 End: 449059 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
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| Family | Start | End | Evalue |
| GT20 | 74 | 541 | 0 |
| KQRLLVVANRLPVSAVRKGEDSWSLDISAGGLVSALLGVTDFEARWIGWAGVNVPDEIGQKALTKALAEKRCIPVFLDEETVHLYYNGYCNNILWPLFHY LGLPQEDRLATTRSFQSQFAAYKKANQMFADVVIEHYEEGDIIWCHDYHLMFLPKCLKDYNSQMKVGWFLHTPFPSSEIHRTLPSRSELLRAVLCADLVG FHTYDYARHFVSACARILGLEGTPEGVEDQGRLTRVAAFPIGIDSERFTRALEVPKVQEYIRELKERFAGRVVMLGVDRLDMIKGIPQKILAFEKFLEDS PEWRDKVVLLQIAVPTRTEVAEYQKLTSQVHEIVGRVNGRFGTLTAVPIHHLDRSLDFAALCAIYAITDVALVTSLRDGMNLVSYEFVACQNSKKGVLIL SEFAGAAQSLGAGAILINPWNITEVAAAIGQALNMPAEEREKRHHHNFVHVTTHTAQKWAEVFVSELN | |||
| Full Sequence |
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| Protein Sequence Length: 909 Download |
| MARNNHNDKS QVPNTRVGRL LRERELRRSG RTSDATDGIR GLEISDIDSS SGSSSTEHNV 60 ESEESESPER RPIKQRLLVV ANRLPVSAVR KGEDSWSLDI SAGGLVSALL GVTDFEARWI 120 GWAGVNVPDE IGQKALTKAL AEKRCIPVFL DEETVHLYYN GYCNNILWPL FHYLGLPQED 180 RLATTRSFQS QFAAYKKANQ MFADVVIEHY EEGDIIWCHD YHLMFLPKCL KDYNSQMKVG 240 WFLHTPFPSS EIHRTLPSRS ELLRAVLCAD LVGFHTYDYA RHFVSACARI LGLEGTPEGV 300 EDQGRLTRVA AFPIGIDSER FTRALEVPKV QEYIRELKER FAGRVVMLGV DRLDMIKGIP 360 QKILAFEKFL EDSPEWRDKV VLLQIAVPTR TEVAEYQKLT SQVHEIVGRV NGRFGTLTAV 420 PIHHLDRSLD FAALCAIYAI TDVALVTSLR DGMNLVSYEF VACQNSKKGV LILSEFAGAA 480 QSLGAGAILI NPWNITEVAA AIGQALNMPA EEREKRHHHN FVHVTTHTAQ KWAEVFVSEL 540 NDTVIEAQQR IRIVPPQLLA GDAIRRYLES DNRLLILGFN AALTEPYDIP ERRGRDQIKE 600 MELKLHPELK QPLTMLCSDP KTTVLILSGS DRNVLDDNFW EHDLWLAAEH GMFLRSTKGD 660 WMTTMPENLN MDWVDSVKHV FEYFTERTPR SIFEERHTSL VWNYKYSDVE FGRLQAMDML 720 QHLWTGPISN SSVDVVQGSR SVEVRAVGVT KGAAIDRIIG EVVHSKGMSS PIDYVLCIGH 780 FLERDEDIYT FFEPELPPDS MGGSRSKSTD STSRHGSEKR PTPPKLQTVG RSNSSRRSSG 840 GSSSGSGRRS TQEETAWNVV DLKSENYFSC AVARTRTHAR YLINSSEDVV SFIKEIAAAG 900 ASPSNSSY* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 70 | 902 | 845 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 74 | 906 | 833 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PRK14501 | PRK14501 | 0 | 76 | 800 | 732 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 76 | 542 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 0 | 75 | 542 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABO61742.1 | 0 | 33 | 906 | 37 | 926 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
| EMBL | CBI20106.1 | 0 | 1 | 902 | 1 | 879 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002285632.1 | 0 | 1 | 902 | 1 | 926 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002331300.1 | 0 | 1 | 899 | 1 | 920 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002531237.1 | 0 | 1 | 901 | 1 | 924 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 76 | 553 | 3 | 467 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 76 | 553 | 3 | 467 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 76 | 553 | 3 | 467 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 76 | 553 | 3 | 467 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1uqu_B | 0 | 76 | 553 | 3 | 467 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| CV711307 | 293 | 74 | 366 | 0 |
| CX546763 | 292 | 74 | 365 | 0 |
| DW069910 | 288 | 162 | 449 | 0 |
| ES813375 | 361 | 437 | 797 | 0 |
| CB651133 | 281 | 102 | 382 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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