Basic Information | |
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Species | Mimulus guttatus |
Cazyme ID | mgv1a001722m |
Family | GH85 |
Protein Properties | Length: 770 Molecular Weight: 85896.7 Isoelectric Point: 6.7402 |
Chromosome | Chromosome/Scaffold: 119 Start: 321603 End: 325315 |
Description | Glycosyl hydrolase family 85 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH85 | 80 | 360 | 0 |
MAGGYTDDKYVQGGTNADAYAIWHWYLIDVFVYFSHNLVALPPPCWTNTAHKHGVKVLGTFIMEGEDGDKQATKLLATKTSAQMYAERLTELAVAMGFDG WLINMEVDLVRAQVPVLEEFVSHLSQTMHSSLPGSLVIWYDSVTVDGFLDWQNKLNDKNKAFFDRCDGIFLNYSWEEEYAKQSADVAGDRKFDVYMGIDV FGRSTFGGGQWTTNVALDVIKKNNISAAIFAPGWVYETKQPPDFQTAQNRWWGLVEKSWGTVPKYPKLLPFYSNFDQGRGY |
Full Sequence |
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Protein Sequence Length: 770 Download |
MRRHSFSATT DAPAPPPFNP STPAVPISYP LKTLDELESR AYFKSFHFPF NQSSVKLPAP 60 AGGADGSLPQ RPRILVCHDM AGGYTDDKYV QGGTNADAYA IWHWYLIDVF VYFSHNLVAL 120 PPPCWTNTAH KHGVKVLGTF IMEGEDGDKQ ATKLLATKTS AQMYAERLTE LAVAMGFDGW 180 LINMEVDLVR AQVPVLEEFV SHLSQTMHSS LPGSLVIWYD SVTVDGFLDW QNKLNDKNKA 240 FFDRCDGIFL NYSWEEEYAK QSADVAGDRK FDVYMGIDVF GRSTFGGGQW TTNVALDVIK 300 KNNISAAIFA PGWVYETKQP PDFQTAQNRW WGLVEKSWGT VPKYPKLLPF YSNFDQGRGY 360 HICIDGEQVF STPWNNISSQ GFQPLIEYPG DSPTQPIQVS VNLKEASYSG GGNIVFRGTL 420 ALKAEFTARL FQGELLLGNS PLHITYSVKS SENSMLGLAL KTSSANNEKT SVFLAASENT 480 SLTIMNQFSK VIMPHKVTNL GADQPGWVMQ ETSIQMKGQV LEEIRAVCYK SNPEKSSSSE 540 YYAILGDIKI TSSGQTPTFP PSSSWIVDGQ FVNWTSDPKG SKHLSVKITW KLKDGNNAAD 600 IFTKYTIYVE KLTKEYEYIG VAAVQSYYIS DLEVTAGTSS LKFIVQACGL DGACQKLEDS 660 PFFLLQVQGN KYEMEKCSRY KCGRILTIKT IGCSERLFYC CSVHGFSCNS ENGDEKGYNG 720 PKIMQKDDSV EVPTYKTRVV LRVVYMLLTV NLFILLVYMS SRLFLKYQK* |
Functional Domains Download unfiltered results here | ||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
cd00598 | GH18_chitinase-like | 0.0002 | 80 | 250 | 183 | + The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model. |
COG4724 | COG4724 | 2.0e-38 | 50 | 472 | 457 | + Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism] |
pfam03644 | Glyco_hydro_85 | 9.0e-119 | 80 | 361 | 301 | + Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates. |
cd06547 | GH85_ENGase | 9.0e-128 | 75 | 388 | 334 | + Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model. |
Gene Ontology | |
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GO Term | Description |
GO:0005737 | cytoplasm |
GO:0033925 | mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | XP_002273683.1 | 0 | 12 | 669 | 10 | 689 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002315137.1 | 0 | 7 | 669 | 9 | 698 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002315138.1 | 0 | 15 | 669 | 18 | 695 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002520781.1 | 0 | 15 | 669 | 16 | 692 | endo beta n-acetylglucosaminidase, putative [Ricinus communis] |
RefSeq | XP_002520784.1 | 0 | 7 | 669 | 9 | 686 | endo beta n-acetylglucosaminidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3fha_D | 1e-26 | 49 | 461 | 28 | 463 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
PDB | 3fha_C | 1e-26 | 49 | 461 | 28 | 463 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
PDB | 3fha_B | 1e-26 | 49 | 461 | 28 | 463 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
PDB | 3fha_A | 1e-26 | 49 | 461 | 28 | 463 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
PDB | 3fhq_F | 1e-26 | 103 | 461 | 83 | 463 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |