| Basic Information | |
|---|---|
| Species | Mimulus guttatus |
| Cazyme ID | mgv1a002612m |
| Family | PL4 |
| Protein Properties | Length: 655 Molecular Weight: 73665.1 Isoelectric Point: 6.602 |
| Chromosome | Chromosome/Scaffold: 3 Start: 2327236 End: 2333658 |
| Description | Rhamnogalacturonate lyase family protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| PL4 | 21 | 623 | 0 |
| VELLRDDQHVVMSNGIVSIRLTVPGGAVTNITYNGSDNLLLPEDKEADRGHWNIVWNMTERGKIIDNLEGTSYKVIVQNENQTEISFTRTWTVGSTNAPL NVDKRFVMLRDSPGFYSYTVLERLKGWPVFDLENGRMVFKLDGNMFDYMAMSDVRQRYMPTSEDRENGEKLEYKEAVLLTNPSNPDFKGEVDDKYLYSCD NKDNKVHGWVSSNLSSGFWMITPSNEFRSGGPLKQDLTSHVGPTVLSVFISTHYAGVDLALKFESQEYWKKVLGPVFIYLNSDASAKTNPSVLWSDAKKR MLEEVASWPYSFPNSKEFVKSEQRGALSGQLLVQDRFVHKKAAPASSSYVGLAPLGKAGSWQFESKGYQFWTQTDEDGSFSIKNVIPGNYSLFAWVPGFV GDYKHASDITITPGSNIQATNVVFDAPRNGPTLWELGIPDRSAAEFFIPDPDSRFKIHQYKQPVEKFRQYGLWARYGELYPQKDLVFTVGSSNYSQDWFF SQVTRNVGGQYVGTTWQILFDLKDVDKTGNYTLQLALASIHYAELQVRFNNPKSAPHFTTGVIGKDNAIARHGIRGLYRFYTIPVPGSSLLVGTNTIFLT QTQ | |||
| Full Sequence |
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| Protein Sequence Length: 655 Download |
| MMISKMNFTS VAVSDSPSPG VELLRDDQHV VMSNGIVSIR LTVPGGAVTN ITYNGSDNLL 60 LPEDKEADRG HWNIVWNMTE RGKIIDNLEG TSYKVIVQNE NQTEISFTRT WTVGSTNAPL 120 NVDKRFVMLR DSPGFYSYTV LERLKGWPVF DLENGRMVFK LDGNMFDYMA MSDVRQRYMP 180 TSEDRENGEK LEYKEAVLLT NPSNPDFKGE VDDKYLYSCD NKDNKVHGWV SSNLSSGFWM 240 ITPSNEFRSG GPLKQDLTSH VGPTVLSVFI STHYAGVDLA LKFESQEYWK KVLGPVFIYL 300 NSDASAKTNP SVLWSDAKKR MLEEVASWPY SFPNSKEFVK SEQRGALSGQ LLVQDRFVHK 360 KAAPASSSYV GLAPLGKAGS WQFESKGYQF WTQTDEDGSF SIKNVIPGNY SLFAWVPGFV 420 GDYKHASDIT ITPGSNIQAT NVVFDAPRNG PTLWELGIPD RSAAEFFIPD PDSRFKIHQY 480 KQPVEKFRQY GLWARYGELY PQKDLVFTVG SSNYSQDWFF SQVTRNVGGQ YVGTTWQILF 540 DLKDVDKTGN YTLQLALASI HYAELQVRFN NPKSAPHFTT GVIGKDNAIA RHGIRGLYRF 600 YTIPVPGSSL LVGTNTIFLT QTQIQSPFVE IMYDYIRLEG PTSKLVDGLF AKSK* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam13620 | CarboxypepD_reg | 3.0e-5 | 392 | 444 | 53 | + Carboxypeptidase regulatory-like domain. | ||
| cd10316 | RGL4_M | 4.0e-31 | 343 | 438 | 96 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10317 | RGL4_C | 7.0e-47 | 454 | 639 | 188 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10320 | RGL4_N | 2.0e-63 | 26 | 308 | 290 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| pfam06045 | Rhamnogal_lyase | 3.0e-71 | 26 | 211 | 189 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_179847.1 | 0 | 4 | 641 | 36 | 674 | lyase [Arabidopsis thaliana] |
| RefSeq | XP_002308510.1 | 0 | 14 | 641 | 40 | 671 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002527245.1 | 0 | 30 | 642 | 2 | 619 | lyase, putative [Ricinus communis] |
| RefSeq | XP_002527356.1 | 0 | 29 | 641 | 127 | 746 | lyase, putative [Ricinus communis] |
| RefSeq | XP_002527356.1 | 2e-30 | 88 | 211 | 1 | 128 | lyase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3njx_A | 0.0002 | 389 | 619 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
| PDB | 1nkg_A | 0.0002 | 389 | 619 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
| PDB | 3njv_A | 0.0002 | 389 | 619 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
| PDB | 2xhn_B | 0.0002 | 389 | 619 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
| PDB | 2xhn_A | 0.0002 | 389 | 619 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| GR163924 | 247 | 91 | 335 | 0 |
| GR153116 | 241 | 207 | 447 | 0 |
| GR150158 | 254 | 168 | 419 | 0 |
| DY969340 | 323 | 239 | 559 | 0 |
| GR187349 | 258 | 382 | 638 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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