y
Basic Information | |
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Species | Mimulus guttatus |
Cazyme ID | mgv1a002627m |
Family | GH32 |
Protein Properties | Length: 653 Molecular Weight: 72784.3 Isoelectric Point: 5.8634 |
Chromosome | Chromosome/Scaffold: 53 Start: 716989 End: 722281 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 133 | 448 | 0 |
HFQPRKNWMNGPLIYKGWYHFFYQYNPYGAVWGNIVWGHAVSKDLIHWRHLPIAMVPDNWYDINGVWTGSATILEDGQLIMLYTGSTNESVQVQNLAYPS DLSDPLLVDWVKYPGNPVLGPPPGIAPQDFRDPTTAWLTPEGKWRFTIGSQINKTGISLVYDTKDFKTFELLNGALHRVAGTGMWECVDFYPVSKVVENG LDTSDNGPEVKHIVKTSLDDDRNDYYAIGTYDGESGSWIPDDPTIDVGIGLRYDYGYFYASKSFYDQEKKRRVLWGWIKETDNEKTDIQKGWASVQAIPR TIVFDKKTGSNIVQWP |
Full Sequence |
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Protein Sequence Length: 653 Download |
MSFQEQGHAL PQSHDTTPIL NSSLCPYTPL LESADANAAA DAPLRQRRRR RTSSVVLLLL 60 TGLLGGAIFV AVVLLKNGNN EPNEEMAVPL VNAVPVGRGV AEGVSSKSFR PLLRAPVFAW 120 TAKMLAWHRT AFHFQPRKNW MNGPLIYKGW YHFFYQYNPY GAVWGNIVWG HAVSKDLIHW 180 RHLPIAMVPD NWYDINGVWT GSATILEDGQ LIMLYTGSTN ESVQVQNLAY PSDLSDPLLV 240 DWVKYPGNPV LGPPPGIAPQ DFRDPTTAWL TPEGKWRFTI GSQINKTGIS LVYDTKDFKT 300 FELLNGALHR VAGTGMWECV DFYPVSKVVE NGLDTSDNGP EVKHIVKTSL DDDRNDYYAI 360 GTYDGESGSW IPDDPTIDVG IGLRYDYGYF YASKSFYDQE KKRRVLWGWI KETDNEKTDI 420 QKGWASVQAI PRTIVFDKKT GSNIVQWPVE EIKRLRMNKN VFEKVEAKPG SIVYIDVPSA 480 SQVDIVVEFE LDEEALKSIN GTDDDEAYSC MTSGGAAERG ALGPFGLLIH THKSLTEQTP 540 VYFYISKDTQ GNFKTFFCAD HSRSSEASEV DKAIYGSTVP VLKGEKLSMR ILVDHSIVES 600 FAQGGRTCIT SRIYPTKAIY EDGHIFLFNN ATDAAITASL QIWQMSSAYA HS* 660 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-59 | 128 | 615 | 511 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 2.0e-66 | 128 | 616 | 511 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 1.0e-91 | 139 | 451 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 2.0e-144 | 133 | 448 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 5.0e-180 | 133 | 605 | 486 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAB47172.1 | 0 | 80 | 649 | 83 | 654 | vacuolar invertase 2, GIN2 [Vitis vinifera=grape berries, Sultana, berries, Peptide, 664 aa] |
EMBL | CAA47636.1 | 0 | 23 | 648 | 6 | 639 | soluble beta-fructosidase [Daucus carota] |
EMBL | CAD12104.1 | 0 | 73 | 649 | 67 | 645 | beta-fructofuranosidase [Cichorium intybus] |
EMBL | CAJ19056.1 | 0 | 25 | 652 | 12 | 651 | invertase [Solanum lycopersicum] |
RefSeq | XP_002272774.1 | 0 | 80 | 649 | 23 | 594 | PREDICTED: similar to vacuolar invertase 2, GIN2 isoform 1 [Vitis vinifera] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 118 | 649 | 8 | 540 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt85h2- Insights Into The Structural Basis Of A Multifunctional (Iso) Flavonoid Glycosyltransferase |
PDB | 3ugh_A | 0 | 118 | 649 | 8 | 540 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt85h2- Insights Into The Structural Basis Of A Multifunctional (Iso) Flavonoid Glycosyltransferase |
PDB | 3ugg_B | 0 | 118 | 649 | 8 | 540 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt85h2- Insights Into The Structural Basis Of A Multifunctional (Iso) Flavonoid Glycosyltransferase |
PDB | 3ugg_A | 0 | 118 | 649 | 8 | 540 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt85h2- Insights Into The Structural Basis Of A Multifunctional (Iso) Flavonoid Glycosyltransferase |
PDB | 3ugf_B | 0 | 118 | 649 | 8 | 540 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |