| Basic Information | |
|---|---|
| Species | Mimulus guttatus |
| Cazyme ID | mgv1a023316m |
| Family | PL4 |
| Protein Properties | Length: 631 Molecular Weight: 71396.4 Isoelectric Point: 6.8098 |
| Chromosome | Chromosome/Scaffold: 3 Start: 2291500 End: 2295389 |
| Description | Rhamnogalacturonate lyase family protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| PL4 | 18 | 611 | 0 |
| VQLLQDDEHVVISNGIVSIRLTVPGGSVTNITYKGSDNVLFPENNEADRGHWDIVWNKTAHADQTDNFVGTNYKVIMQNENQTEISFTRTWTFNSTKAPL NVDKRYVMLKDSPGFYTYVVLERLPGWPVFYIQEGRVLFKLDENMFQYMAVSDARQRFMPKNQDRRNGQVLGYKEAVLITNATNPAFNGEVDDKYLYSND NKDIKVHGWVGSNPSLGFWMITPSNEFRTGGPLKQDLTTHLGPTVLHYFTSTHYAGEVLAIKFKTEEYWKKVIGPVYIYLNSNDSANADPSLLWIDAKQR MEKEEASWPYEFALSEDFVKSNQRGALNGQLLVRDWFVSKQDVPGASAYVGLALPGEAGSWHYENKGYQFWTKTDENGSFSIKNVIPGNYSLFAWVPGFV GDYKHASNITITPGSNIQATNVVFVPPRNGSTLWEIGIPDRTAAEFFIPDPSPTYKMWRQYGLWARYKDIHPVTDQVYTVGLSDYKRDWFFAHVPRNVGP KLYNATTWQIIFEIKNITKGSYTLQLALASANGAEVQVRVNNPESEELFTTGGIGKDNTIARHGIHGRYHLYSIGIADTHLVVGNNTIFLTQTR | |||
| Full Sequence |
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| Protein Sequence Length: 631 Download |
| KRLIQDNIIR ADLPSLGVQL LQDDEHVVIS NGIVSIRLTV PGGSVTNITY KGSDNVLFPE 60 NNEADRGHWD IVWNKTAHAD QTDNFVGTNY KVIMQNENQT EISFTRTWTF NSTKAPLNVD 120 KRYVMLKDSP GFYTYVVLER LPGWPVFYIQ EGRVLFKLDE NMFQYMAVSD ARQRFMPKNQ 180 DRRNGQVLGY KEAVLITNAT NPAFNGEVDD KYLYSNDNKD IKVHGWVGSN PSLGFWMITP 240 SNEFRTGGPL KQDLTTHLGP TVLHYFTSTH YAGEVLAIKF KTEEYWKKVI GPVYIYLNSN 300 DSANADPSLL WIDAKQRMEK EEASWPYEFA LSEDFVKSNQ RGALNGQLLV RDWFVSKQDV 360 PGASAYVGLA LPGEAGSWHY ENKGYQFWTK TDENGSFSIK NVIPGNYSLF AWVPGFVGDY 420 KHASNITITP GSNIQATNVV FVPPRNGSTL WEIGIPDRTA AEFFIPDPSP TYKMWRQYGL 480 WARYKDIHPV TDQVYTVGLS DYKRDWFFAH VPRNVGPKLY NATTWQIIFE IKNITKGSYT 540 LQLALASANG AEVQVRVNNP ESEELFTTGG IGKDNTIARH GIHGRYHLYS IGIADTHLVV 600 GNNTIFLTQT RKLSPFSGVM YDYLRLEGPA * 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam13620 | CarboxypepD_reg | 4.0e-5 | 389 | 442 | 54 | + Carboxypeptidase regulatory-like domain. | ||
| cd10316 | RGL4_M | 5.0e-29 | 340 | 435 | 96 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10317 | RGL4_C | 1.0e-45 | 451 | 627 | 180 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10320 | RGL4_N | 1.0e-58 | 23 | 306 | 284 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| pfam06045 | Rhamnogal_lyase | 3.0e-66 | 23 | 208 | 189 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAS99719.1 | 0 | 12 | 629 | 46 | 674 | At2g22620 [Arabidopsis thaliana] |
| RefSeq | NP_179847.1 | 0 | 12 | 629 | 46 | 674 | lyase [Arabidopsis thaliana] |
| RefSeq | XP_002308510.1 | 0 | 11 | 629 | 40 | 671 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002527245.1 | 0 | 27 | 630 | 2 | 619 | lyase, putative [Ricinus communis] |
| RefSeq | XP_002527353.1 | 0 | 13 | 629 | 1 | 633 | lyase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1nkg_A | 0.002 | 272 | 607 | 166 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
| PDB | 3njv_A | 0.002 | 272 | 607 | 166 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
| PDB | 2xhn_B | 0.002 | 272 | 607 | 166 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
| PDB | 2xhn_A | 0.002 | 272 | 607 | 166 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
| PDB | 3njx_A | 0.003 | 272 | 607 | 166 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| GR187349 | 257 | 379 | 626 | 0 |
| GR153116 | 241 | 204 | 444 | 0 |
| GR150158 | 255 | 164 | 416 | 0 |
| GR163924 | 247 | 88 | 332 | 0 |
| DY969340 | 324 | 236 | 548 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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