Basic Information | |
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Species | Mimulus guttatus |
Cazyme ID | mgv1a024929m |
Family | GT64 |
Protein Properties | Length: 775 Molecular Weight: 85818.4 Isoelectric Point: 9.2796 |
Chromosome | Chromosome/Scaffold: 179 Start: 47503 End: 51592 |
Description | glycosyltransferase family protein 47 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT64 | 527 | 761 | 0 |
FTMLTMTFDARLWNLKMYVKHYSRCASVREIVVVWNKGTPPLQSDFDSAVPVRIRVEEKNSLNNRFKADPAIKTRGVLELDDDIMMTCDDVERGFRVWRE RPDRIVGFYPRFVGGGGRLRYRGEKYARGHNGYNMILTGAAFIDGGAAFERYWSEEARAGRALVDGFFNCEDVLMNYLYANGSSSSVVEYVRPTWAVDTS KFSGVAISRNTQAHYGVRSNCLKKFSDMYGSLGDR |
Full Sequence |
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Protein Sequence Length: 775 Download |
MGSGPIVSSG GGGGWSRWFH DSNGGGKSSN GGAARNGNHN NNNNNNEACV FSSTLAYFLL 60 SLAVLGSIGI LYARFMITAN VRTGITAVGC QQDNEGSWAI GIFYGDSPFS LKPIEDINVW 120 KNKSAAWPVA NPIITCASLS DAGSPSNFVA DPFLYVQGDI LYVFYETKNS ITMQGDIGVS 180 RSLDKGATWQ QLGIALDEDW HLSYPYVFEY NGNIYMMPEG STKGDLRLYR STDFPLKWTL 240 EKVIMKKPLV DSFMIPHKGK FYLFGSDHSG FGTKKNGHLS IWHSVSPLGP WKPHKKNPVH 300 NTHKSVGARN GGRPFVHNGN LYRIGQDCGD TYGRRIRVFK ISVLTTEDFE EIEVPSNIEE 360 SLKGRNAWNG ARHHHLDVQR LSSGEWVAVI DGDRVPSGDS VRRSVFGFGA VIAVGALVVL 420 VGMVVGAVKC LVPLSWCPHS MGKRSDSFLA WERPSLLSSK IRLLCSRLNR ASSIVHARVR 480 PNTCTGVLFL VLAVVAAAAL TCTGVKYLYG RSGAEEPYPL DGSYSQFTML TMTFDARLWN 540 LKMYVKHYSR CASVREIVVV WNKGTPPLQS DFDSAVPVRI RVEEKNSLNN RFKADPAIKT 600 RGVLELDDDI MMTCDDVERG FRVWRERPDR IVGFYPRFVG GGGRLRYRGE KYARGHNGYN 660 MILTGAAFID GGAAFERYWS EEARAGRALV DGFFNCEDVL MNYLYANGSS SSVVEYVRPT 720 WAVDTSKFSG VAISRNTQAH YGVRSNCLKK FSDMYGSLGD RKLEFNRRKD GWDV* 780 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd08978 | GH_F | 0.003 | 117 | 298 | 196 | + Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F. This glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) includes family 43 (GH43) and 62 (GH62). GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH62 includes enzymes characterized as arabinofuranosidases (alpha-L-arabinofuranosidases; EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose side chains from xylans. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. GH62 are also predicted to be inverting enzymes. A common structural feature of both, GH43 and GH62 enzymes, is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
cd08996 | GH32_B_Fructosidase | 0.002 | 156 | 282 | 147 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam09258 | Glyco_transf_64 | 1.0e-67 | 527 | 756 | 240 | + Glycosyl transferase family 64 domain. Members of this family catalyze the transfer reaction of N-acetylglucosamine and N-acetylgalactosamine from the respective UDP-sugars to the non-reducing end of [glucuronic acid]beta 1-3[galactose]beta 1-O-naphthalenemethanol, an acceptor substrate analog of the natural common linker of various glycosylaminoglycans. They are also required for the biosynthesis of heparan-sulphate. |
Gene Ontology | |
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GO Term | Description |
GO:0016758 | transferase activity, transferring hexosyl groups |
GO:0031227 | intrinsic to endoplasmic reticulum membrane |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAB85556.1 | 0 | 21 | 774 | 6 | 764 | putative protein [Arabidopsis thaliana] |
RefSeq | NP_196070.2 | 0 | 21 | 774 | 7 | 765 | glycosyltransferase family protein 47 [Arabidopsis thaliana] |
RefSeq | XP_002262646.1 | 0 | 85 | 773 | 68 | 755 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002315348.1 | 0 | 75 | 774 | 87 | 847 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002532924.1 | 0 | 84 | 774 | 15 | 704 | transferase, transferring glycosyl groups, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1on8_B | 3e-25 | 519 | 758 | 22 | 270 | A Chain A, Family 89 Glycoside Hydrolase From Clostridium Perfringens In Complex With 2-Acetamido-1,2-Dideoxynojirmycin |
PDB | 1on8_A | 3e-25 | 519 | 758 | 22 | 270 | A Chain A, Family 89 Glycoside Hydrolase From Clostridium Perfringens In Complex With 2-Acetamido-1,2-Dideoxynojirmycin |
PDB | 1on6_B | 3e-25 | 519 | 758 | 22 | 270 | A Chain A, Family 89 Glycoside Hydrolase From Clostridium Perfringens In Complex With 2-Acetamido-1,2-Dideoxynojirmycin |
PDB | 1on6_A | 3e-25 | 519 | 758 | 22 | 270 | A Chain A, Family 89 Glycoside Hydrolase From Clostridium Perfringens In Complex With 2-Acetamido-1,2-Dideoxynojirmycin |
PDB | 1omz_B | 3e-25 | 519 | 758 | 22 | 270 | A Chain A, Family 89 Glycoside Hydrolase From Clostridium Perfringens In Complex With 2-Acetamido-1,2-Dideoxynojirmycin |