Basic Information | |
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Species | Fragaria vesca |
Cazyme ID | mrna08646.1-v1.0-hybrid |
Family | GH13 |
Protein Properties | Length: 801 Molecular Weight: 91478.1 Isoelectric Point: 6.9436 |
Chromosome | Chromosome/Scaffold: 2 Start: 19466608 End: 19474637 |
Description | starch branching enzyme 2.2 |
View CDS |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 288 | 605 | 4.1e-28 |
HYNTVQLMAVMEHSHYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHSLGLRVLMDVIHSHASNNVTDGLIDFEVGQCTRDSYFHACDRGYHKLWDSRLF NYANWEVLRFFLSNLRWWLEEYQFDGFRFDGVTSMLYHHHGINVAFTGDYNEYFSEATDVDAVVYLMLANYVIHKILPSATVIAEDASGMPGLGRPVSEG GVGFDYRLAMAIPDKWIDYVKNKKDEEWSVTEISWSLTNRRYTEKCVSYAESHDQSIVGDKTIASFLMDKEMYSGMSCLKDASPTVDRGIALHKMIHFIT MALGGEGYLNFMGSEFGN |
Full Sequence |
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Protein Sequence Length: 801 Download |
MLGSLGRLHL TYSSSSGSFS SSKDYPSPRL SHPKLPSFPL LRHRALCPPT NLRCGKLERD 60 CKVRAVLTDD SSIMTVNEGE MEDIGVLKTD SNLEPYKDHF KCRIKRYLEQ KRLFEKYEGG 120 LEEFARGFVK FGFNREDGGV VYREWAPLAQ EAQLVGDFNG WEGSNHKMEK NQFGVWSIKI 180 HDFGGNPAIP HNSKVKFRSK DRNGVWVDRI PAWIKYATAD PAKFAAPYDG VYWDPPPTER 240 YQFKYGRPPK PKAPRIYEAH VGMSSSEPRI SSYREFADNV LPRIRANHYN TVQLMAVMEH 300 SHYASFGYHV TNFFAVSSRS GTPEDLKYLI DKAHSLGLRV LMDVIHSHAS NNVTDGLIDF 360 EVGQCTRDSY FHACDRGYHK LWDSRLFNYA NWEVLRFFLS NLRWWLEEYQ FDGFRFDGVT 420 SMLYHHHGIN VAFTGDYNEY FSEATDVDAV VYLMLANYVI HKILPSATVI AEDASGMPGL 480 GRPVSEGGVG FDYRLAMAIP DKWIDYVKNK KDEEWSVTEI SWSLTNRRYT EKCVSYAESH 540 DQSIVGDKTI ASFLMDKEMY SGMSCLKDAS PTVDRGIALH KMIHFITMAL GGEGYLNFMG 600 SEFGNPEWID FPRDGNGWSY EKCRRQWNLV DTDHLRYKFM NAFDRAMNLL DEKFSFLSST 660 KQIVSSTNEE DKVIVFERGD LVFVFNFHPV NTYDRYKVGC DLPGKYRVAL DSDAFEFGGH 720 GRVGHDVDHF TFPEGIPGVP GTNFNRRPNS FRVLSPPRTS VVYYRVENST AKVVVGQEHK 780 EVGSMKHENV GPLVNLSSDN * |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02960 | PLN02960 | 8.0e-7 | 103 | 182 | 86 | + alpha-amylase | ||
PLN03244 | PLN03244 | 1.0e-135 | 187 | 717 | 536 | + alpha-amylase; Provisional | ||
PLN02447 | PLN02447 | 0 | 26 | 773 | 748 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 0 | 237 | 645 | 409 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02960 | PLN02960 | 0 | 187 | 713 | 537 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3aml_A | 0 | 73 | 776 | 1 | 703 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3amk_A | 0 | 73 | 767 | 1 | 694 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 73 | 767 | 1 | 694 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 73 | 767 | 1 | 694 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3k1d_A | 9.94922e-44 | 133 | 761 | 128 | 717 | A Chain A, Crystal Structure Of Glycogen Branching Enzyme Synonym: 1,4- Glucan:1,4-Alpha-D-Glucan 6-Glucosyl-Transferase From Mycob Tuberculosis H3 |
Sequence Alignments (This image is cropped. Click for full image.) |
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