Basic Information | |
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Species | Fragaria vesca |
Cazyme ID | mrna21228.1-v1.0-hybrid |
Family | GH32 |
Protein Properties | Length: 642 Molecular Weight: 71158.8 Isoelectric Point: 4.431 |
Chromosome | Chromosome/Scaffold: 7 Start: 19049655 End: 19053121 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 122 | 437 | 0 |
HFQPEENWMNGPLFHKGWYHLFYQYNPDSAVWGNITWGHAVSTDLIHWLYLPLAMVADQWYDANGVWSGSATLLPDGNIVMLYTGDTVDAVQVQCLAYPG NLSDPLLLDWVKYSGNPVLTPPPGILSTDFRDPTTAWIGPDGKWRITIGSKINTTGVSFVYITEDFINYNMSQGLLHEVPGTGMWECIDFYPVAINGSKG LDTSVNDPSVKHVLKASLDDTKVDHYALGTYFIENETWVPDNPALDVGIGLKYDYGRYYASKTFYDQNKERRILWGWINETDTESDDLAKGWASVQTIPR SVLFDNKTGTNLIQWP |
Full Sequence |
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Protein Sequence Length: 642 Download |
MVDTSDSSYA PLPGEEPLFS GHSPTSLRRP WKGFAVILAS VVFLLSLVAL IIHQGPSNVV 60 PEKQDEHQHR PHSTTPASET TSSWEPRGKA LGVSAKSNPP VSDELSYNWT NAMFSWQRTA 120 FHFQPEENWM NGPLFHKGWY HLFYQYNPDS AVWGNITWGH AVSTDLIHWL YLPLAMVADQ 180 WYDANGVWSG SATLLPDGNI VMLYTGDTVD AVQVQCLAYP GNLSDPLLLD WVKYSGNPVL 240 TPPPGILSTD FRDPTTAWIG PDGKWRITIG SKINTTGVSF VYITEDFINY NMSQGLLHEV 300 PGTGMWECID FYPVAINGSK GLDTSVNDPS VKHVLKASLD DTKVDHYALG TYFIENETWV 360 PDNPALDVGI GLKYDYGRYY ASKTFYDQNK ERRILWGWIN ETDTESDDLA KGWASVQTIP 420 RSVLFDNKTG TNLIQWPVEE IEELRLNSTD FSDVLVEAGA VVELDIGTAT QLDIFVEFEI 480 ESLGSETLNS SVGCGGGATD RTSFGPFGIL VIADEILSEL TPIYFNIANS TEGDVTTYFC 540 ADERRSSKAP DVFKQVYGSE VPVLEGEKHF ARVLVDHSIV ESFAQGGRTV ITSRIYPTEA 600 IYGAAKLFLF NNATGLNVKA TLKIWQLNSA FIHPFPLDQV Q* |
Functional Domains Download unfiltered results here | ||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
TIGR01322 | scrB_fam | 3.0e-48 | 118 | 597 | 506 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. |
COG1621 | SacC | 6.0e-67 | 118 | 629 | 531 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] |
cd08996 | GH32_B_Fructosidase | 4.0e-85 | 128 | 440 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. |
pfam00251 | Glyco_hydro_32N | 1.0e-139 | 122 | 437 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. |
smart00640 | Glyco_32 | 3.0e-174 | 122 | 587 | 479 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 107 | 635 | 8 | 544 | B Chain B, Crystal Structure Of Putative Membrane Anchored Protein From Corynebacterium Diphtheriae |
PDB | 3ugh_A | 0 | 107 | 635 | 8 | 544 | B Chain B, Crystal Structure Of Putative Membrane Anchored Protein From Corynebacterium Diphtheriae |
PDB | 3ugg_B | 0 | 107 | 635 | 8 | 544 | B Chain B, Crystal Structure Of Putative Membrane Anchored Protein From Corynebacterium Diphtheriae |
PDB | 3ugg_A | 0 | 107 | 635 | 8 | 544 | B Chain B, Crystal Structure Of Putative Membrane Anchored Protein From Corynebacterium Diphtheriae |
PDB | 3ugf_B | 0 | 107 | 635 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |