| Basic Information | |
|---|---|
| Species | Fragaria vesca |
| Cazyme ID | mrna28570.1-v1.0-hybrid |
| Family | GH13 |
| Protein Properties | Length: 805 Molecular Weight: 90813.6 Isoelectric Point: 7.7983 |
| Chromosome | Chromosome/Scaffold: 3 Start: 21086577 End: 21103114 |
| Description | alpha-amylase-like |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
 | |||
| Family | Start | End | Evalue |
| GH13 | 418 | 722 | 4.4e-38 |
| KGGWYNILEQSAAELASSGITHVWLPPPSQAISDEGYMPGRLYDLTSKYGNQDGLKALVKAYQDNGIKSVADIVINHRCAEKQDERGIWCIFEGGTPDDR LDWGPSLICSDDTKYSDGKGNPDTGADFGGAPDIDHKNTRVQSELSDWMNWLKTEIGFSGWRFDFVRGYAPEFTKLYVANTKPSFSVGEYWNSIAYGSDG KPEYNQDNHRNELAGWIGNAGGEVTAFDFTTKGILQAAVQGELWRLKDSNGGPPGLIGISPRNSVTFIDNHDTGSTQKLWPFPSDKVMQGYAYTLTHPGI PSIFY | |||
| Full Sequence |
|---|
| Protein Sequence Length: 805 Download |
| MAEHLSLEDG WSRDACKKGE AGGGKRLRIT KAEISEEPKF NIKLWFPSEL GQSADRKWKK 60 IAGNRRIADS DFFFRCTEVG AGAHNAAPDS SHFLVAVYFA FGFFFARLFL DRFIFTTFVD 120 NGGCHCRRDW LNQCQLLLQG SRLAIWMSRK GSAPSKIDMV QRAKITKCSE SMWKLTYYAT 180 VEACILSISY QEPWFTDTRE YFKGWPNQEL GLPLKLVYMC QCGFYTYSIV ALLTWETRRK 240 DFSVMMSHHV ITVFLIGMSY ITSFFRIGSI ILALHDGSDV FMEAAKVFKY SEKELAASVF 300 FGCFAFSWLI LRLIYFPFWV ISSSSYDLVK HLDLSLSSHT TLYYLFNTML MTLLVFHIYW 360 WVMICSMVRR QLSNSGKRRG MNSLKISTLC FLLSIISIPN FTVSQILFQG FNWESAKKGG 420 WYNILEQSAA ELASSGITHV WLPPPSQAIS DEGYMPGRLY DLTSKYGNQD GLKALVKAYQ 480 DNGIKSVADI VINHRCAEKQ DERGIWCIFE GGTPDDRLDW GPSLICSDDT KYSDGKGNPD 540 TGADFGGAPD IDHKNTRVQS ELSDWMNWLK TEIGFSGWRF DFVRGYAPEF TKLYVANTKP 600 SFSVGEYWNS IAYGSDGKPE YNQDNHRNEL AGWIGNAGGE VTAFDFTTKG ILQAAVQGEL 660 WRLKDSNGGP PGLIGISPRN SVTFIDNHDT GSTQKLWPFP SDKVMQGYAY TLTHPGIPSI 720 FYDHFFDWGL KGEISKLTAI RSRNGIKPDS AVRILAADAD LYVASIDDKI ITKIGPRFDV 780 GNLVPPTFKI ATSGKDYSVW EKSA* |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
 | ||||||||
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| smart00724 | TLC | 4.0e-47 | 166 | 373 | 211 | + TRAM, LAG1 and CLN8 homology domains. Protein domain with at least 5 transmembrane alpha-helices. Lag1p and Lac1p are essential for acyl-CoA-dependent ceramide synthesis, TRAM is a subunit of the translocon and the CLN8 gene is mutated in Northern epilepsy syndrome. The family may possess multiple functions such as lipid trafficking, metabolism, or sensing. Trh homologues possess additional homeobox domains. | ||
| PLN02784 | PLN02784 | 5.0e-142 | 405 | 803 | 404 | + alpha-amylase | ||
| PLN02361 | PLN02361 | 3.0e-147 | 405 | 802 | 403 | + alpha-amylase | ||
| cd11314 | AmyAc_arch_bac_plant_AmyA | 2.0e-152 | 406 | 752 | 350 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN00196 | PLN00196 | 0 | 381 | 802 | 427 | + alpha-amylase; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004556 | alpha-amylase activity |
| GO:0005509 | calcium ion binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0016021 | integral to membrane |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1bg9_A | 0 | 405 | 802 | 1 | 402 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 1ava_B | 0 | 405 | 802 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 1ava_A | 0 | 405 | 802 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 1amy_A | 0 | 405 | 802 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 2qpu_C | 0 | 405 | 803 | 2 | 405 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
