Basic Information | |
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Species | Fragaria vesca |
Cazyme ID | mrna32247.1-v1.0-hybrid |
Family | GH32 |
Protein Properties | Length: 657 Molecular Weight: 72495.6 Isoelectric Point: 5.784 |
Chromosome | Chromosome/Scaffold: 5 Start: 330608 End: 335011 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 140 | 455 | 0 |
HFQPEKNWMNGPLFYKGWYHFFYQYNPKGAVWGNIVWGHAVSRDLIHWLHLPLAMVADQWYDINGVWTGSATILPNGQIVMLYTGSTNESVQVQCLAYPA DHNDPLLTKWIKYSGNPVLFPPPGIGIKDFRDPTTAWYTKEGKWRITIGSKVNKTGISLVYDTKDFIKYEQLDGVLHAVPGTGMWECIDFYPVSKTSDKG LDTSENGADVKHVIKTSLDDDRNDYYALGSYNEQTGKWVPDNPNIDVGIGIRYDYGKFYASKTFYDQNKQRRVLWGWIGESDSENADVQKGWASLQGIPR TVLFDQKTGSNLLQWP |
Full Sequence |
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Protein Sequence Length: 657 Download |
MADRRPLLPS DPDALPTAYA GSSSSPEIRR IHVKEVLAMF AGVVMVSLLV VIIGNTGHDQ 60 EAGNASHHRN YMPDKPMPLT SSSPTNETAT IPDKLQAVSR GMAAGVSEKS SRLYSSGFGA 120 GTPVYPWNNS MLSWQRTAFH FQPEKNWMNG PLFYKGWYHF FYQYNPKGAV WGNIVWGHAV 180 SRDLIHWLHL PLAMVADQWY DINGVWTGSA TILPNGQIVM LYTGSTNESV QVQCLAYPAD 240 HNDPLLTKWI KYSGNPVLFP PPGIGIKDFR DPTTAWYTKE GKWRITIGSK VNKTGISLVY 300 DTKDFIKYEQ LDGVLHAVPG TGMWECIDFY PVSKTSDKGL DTSENGADVK HVIKTSLDDD 360 RNDYYALGSY NEQTGKWVPD NPNIDVGIGI RYDYGKFYAS KTFYDQNKQR RVLWGWIGES 420 DSENADVQKG WASLQGIPRT VLFDQKTGSN LLQWPVEEIE KLRLNMNNFD RVQVKAGSVV 480 PLDVGTATQL DIVAEFELDQ TVLESTAESN EEFSCQTSGG AGKRGALGPF GLLVLADDGL 540 TERTPVYFYV VKGSGGTVNT YFCADQTRST VANDVVKQVS GSYVPVLKGE KLSVRILVDH 600 SIIDSFAQGG RTTITTRVYP TQAIYGAARL FLFNNATETS VTASLQIDGT PSSEDL* 660 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 2.0e-49 | 136 | 620 | 508 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 4.0e-68 | 136 | 622 | 504 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-89 | 146 | 458 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 4.0e-150 | 140 | 455 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 140 | 610 | 484 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 125 | 647 | 8 | 533 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
PDB | 3ugh_A | 0 | 125 | 647 | 8 | 533 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
PDB | 3ugg_B | 0 | 125 | 647 | 8 | 533 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
PDB | 3ugg_A | 0 | 125 | 647 | 8 | 533 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
PDB | 3ugf_B | 0 | 125 | 647 | 8 | 533 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |