| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g000446m |
| Family | GT24 |
| Protein Properties | Length: 1499 Molecular Weight: 169472 Isoelectric Point: 5.8969 |
| Chromosome | Chromosome/Scaffold: 00219 Start: 228207 End: 248611 |
| Description | UDP-glucose:glycoprotein glucosyltransferases;transferases, transferring hexosyl groups;transferases, transferring glycosyl groups |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT24 | 1339 | 1497 | 0 |
| INIFSIASGHLYERFLKIMILSVLKNTCRPVKFWFIKNYLSPQFKDVIPHMAQEYGFEYELITYKWPTWLHKQKEKQRIIWAYKILFLDVIFPLSLEKVI FVDADQVVRADMGELYDMDIKGRPLAYTPFCDNNKDMDGYRFWRQGFWKDHLRGRPYHI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 1499 Download |
| MEYRFRSGFC VLIILVCVSL CGFASVCAQI QKPKNVQVAV RAKWSGTPLL LEAGELLASE 60 RKDLFWEFIE KWLHSEENDA DSRTAKDCLK RIVRHGSSLL SESLASLFEF SLTLRSASPR 120 LVLYRQLAEE SLSSFPPFDD SNLKNEVGGA SEANEKLETK KSDSLLVGVN PKSPGGKCCW 180 VDTGGALFLE VSELLMWLRS PSELTGESFQ QPELFDFDHI HAESSISSRT AILYGALGSD 240 CFKEFHINLV QAAKEGKVMY VVRPVLPSGC EANVGNCGAV GAKDSLNLGG YGVELALKNM 300 EYKAIDDSMI KEGVTLEDPR TEDLSQEVRG FVFSKLLERK PDLTSEIMSF RDYLLSSTTS 360 ETLEVWELKD LGHQTAQRIV HASDPLQSMQ EISQNFPSVV SSLSRMKLND SIKDEIVANQ 420 RYMPPGKSLM ALNGALINIE DIDLYLLIDL VHQELSLADQ FSKLKIPRTI TQKLLSTVPP 480 AESSMFRVDF RSTHVQYLNN LEEDAMYKRW RSNINEILMP VFPGQLRYIR KNLFHAVYVL 540 DPATVCGLEV IDMIMSLYEN HFPLRFGVIL YSSKFIKSIE INGGELHSPV AEDDSPVNED 600 ISSLIIRLFL FIKESHGTQT AFQFLSNVNR LRMESADSAD DDALEIHHVE GAFVETILPK 660 AKTPPQDMLL KLEKEKTFMD QSQESSMFVF KLGLTKLKCC LLMNGLVSES SEEALLNAMN 720 DELQRIQEQV YYGNINSYTD VLEKVLSESG INRYNPQIIT DAKVKPKFIS LASSFLGRET 780 ELKDINYLHS PETVDDVKPV THLLAVDVTS KKGMKLLHEG IRFLIGGSNG ARLGVLFSAS 840 READLPSIIF VKAFEITAST YSHKKKVLEF LDQLCSFYER TYLLASSATA DSTQAFIDKV 900 CEFAEANGLS SKVYRASLPE YSKGKVRKQL NKVVQFLHRQ LGVESGANAV ITNGRVTFPI 960 DESTFLSHDL SLLESVEFKH RIKHIWEIIE EVNWQETYPD IDPDMLTSKF VSDIILFVTS 1020 SMAMRDRSSE SARFEILSAE YSAVVFNSEN STIHIDAVID PLSPTGQKLS SLLRVLQRYA 1080 QPSMRIVLNP MSSLVDIPLK NYYRYVVPTM DDFSNTDYSI SGPKAFFANM PLSKTLTMNL 1140 DVPEPWLVEP VIAVHDLDNI LLEKLGDTRT LQAVFELEAL VLTGHCSEKD HEPPQGLQLI 1200 LGTKSTPHLV DTLVMANLGY WQMKVSPGVW YLQLAPGRSS ELYVLKEDGN VNEDRSLSKR 1260 ITINDLRGKV VHMEVVKKKG KENEKLLVSS DEDSHSQAEG HWNSNFLKWA SGFIGGSEQS 1320 KKEKAAVDHG KVERHGKTIN IFSIASGHLY ERFLKIMILS VLKNTCRPVK FWFIKNYLSP 1380 QFKDVIPHMA QEYGFEYELI TYKWPTWLHK QKEKQRIIWA YKILFLDVIF PLSLEKVIFV 1440 DADQVVRADM GELYDMDIKG RPLAYTPFCD NNKDMDGYRF WRQGFWKDHL RGRPYHIR* 1500 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam01501 | Glyco_transf_8 | 4.0e-5 | 1343 | 1464 | 128 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd04194 | GT8_A4GalT_like | 1.0e-11 | 1339 | 1464 | 128 | + A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS. | ||
| cd00505 | Glyco_transf_8 | 1.0e-37 | 1339 | 1497 | 160 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| pfam06427 | UDP-g_GGTase | 2.0e-77 | 1012 | 1217 | 212 | + UDP-glucose:Glycoprotein Glucosyltransferase. The N-terminal region of this group of proteins is required for correct folding of the ER UDP-Glc: glucosyltransferase. | ||
| cd06432 | GT8_HUGT1_C_like | 7.0e-108 | 1339 | 1497 | 159 | + The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003980 | UDP-glucose:glycoprotein glucosyltransferase activity |
| GO:0006486 | protein glycosylation |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG51883.1 | 0 | 9 | 1497 | 12 | 1502 | AC016162_4 putative UDP-glucose:glycoprotein glucosyltransferase; 101200-91134 [Arabidopsis thaliana] |
| EMBL | CBI23772.1 | 0 | 1 | 1326 | 1 | 1299 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_177278.3 | 0 | 9 | 1497 | 12 | 1459 | EBS1 (EMS-mutagenized bri1 suppressor 1); UDP-glucose:glycoprotein glucosyltransferase/ transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002268972.1 | 0 | 1 | 1497 | 1 | 1462 | PREDICTED: similar to EBS1 (EMS-MUTAGENIZED BRI1 SUPPRESSOR 1); UDP-glucose:glycoprotein glucosyltransferase/ transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Vitis vinifera] |
| RefSeq | XP_002529534.1 | 0 | 1 | 1497 | 1 | 1460 | UDP-glucose glycoprotein:glucosyltransferase, putative [Ricinus communis] |
