| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g001078m |
| Family | GT24 |
| Protein Properties | Length: 1163 Molecular Weight: 132216 Isoelectric Point: 6.1223 |
| Chromosome | Chromosome/Scaffold: 00219 Start: 226844 End: 241990 |
| Description | UDP-glucose:glycoprotein glucosyltransferases;transferases, transferring hexosyl groups;transferases, transferring glycosyl groups |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT24 | 855 | 1102 | 0 |
| INIFSIASGHLYERFLKIMILSVLKNTCRPVKFWFIKNYLSPQFKDVIPHMAQEYGFEYELITYKWPTWLHKQKEKQRIIWAYKILFLDVIFPLSLEKVI FVDADQVVRADMGELYDMDIKGRPLAYTPFCDNNKDMDGYRFWRQGFWKDHLRGRPYHISALYVVDLKRFRETAAGDNLRVFYETLSKDPNSLANLDQDL PNYAQHTVPIFSLPQEWLWCESWCGNATKSKAKTIDLCNNPMTKEPKL | |||
| Full Sequence |
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| Protein Sequence Length: 1163 Download |
| MFRVDFRSTH VQYLNNLEED AMYKRWRSNI NEILMPVFPG QLRYIRKNLF HAVYVLDPAT 60 VCGLEVIDMI MSLYENHFPL RFGVILYSSK FIKSIEINGG ELHSPVAEDD SPVNEDISSL 120 IIRLFLFIKE SHGTQTAFQF LSNVNRLRME SADSADDDAL EIHHVEGAFV ETILPKAKTP 180 PQDMLLKLEK EKTFMDQSQE SSMFVFKLGL TKLKCCLLMN GLVSESSEEA LLNAMNDELQ 240 RIQEQVYYGN INSYTDVLEK VLSESGINRY NPQIITDAKV KPKFISLASS FLGRETELKD 300 INYLHSPETV DDVKPVTHLL AVDVTSKKGM KLLHEGIRFL IGGSNGARLG VLFSASREAD 360 LPSIIFVKAF EITASTYSHK KKVLEFLDQL CSFYERTYLL ASSATADSTQ AFIDKVCEFA 420 EANGLSSKVY RASLPEYSKG KVRKQLNKVV QFLHRQLGVE SGANAVITNG RVTFPIDEST 480 FLSHDLSLLE SVEFKHRIKH IWEIIEEVNW QETYPDIDPD MLTSKFVSDI ILFVTSSMAM 540 RDRSSESARF EILSAEYSAV VFNSENSTIH IDAVIDPLSP TGQKLSSLLR VLQRYAQPSM 600 RIVLNPMSSL VDIPLKNYYR YVVPTMDDFS NTDYSISGPK AFFANMPLSK TLTMNLDVPE 660 PWLVEPVIAV HDLDNILLEK LGDTRTLQAV FELEALVLTG HCSEKDHEPP QGLQLILGTK 720 STPHLVDTLV MANLGYWQMK VSPGVWYLQL APGRSSELYV LKEDGNVNED RSLSKRITIN 780 DLRGKVVHME VVKKKGKENE KLLVSSDEDS HSQAEGHWNS NFLKWASGFI GGSEQSKKEK 840 AAVDHGKVER HGKTINIFSI ASGHLYERFL KIMILSVLKN TCRPVKFWFI KNYLSPQFKD 900 VIPHMAQEYG FEYELITYKW PTWLHKQKEK QRIIWAYKIL FLDVIFPLSL EKVIFVDADQ 960 VVRADMGELY DMDIKGRPLA YTPFCDNNKD MDGYRFWRQG FWKDHLRGRP YHISALYVVD 1020 LKRFRETAAG DNLRVFYETL SKDPNSLANL DQDLPNYAQH TVPIFSLPQE WLWCESWCGN 1080 ATKSKAKTID LCNNPMTKEP KLQGARRIVS EWPDLDSEAR QFTAKILGEE VVTLETPAPV 1140 GPMQTSGSDA SSKGDLESKA EL* 1200 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam01501 | Glyco_transf_8 | 3.0e-6 | 859 | 1054 | 205 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd04194 | GT8_A4GalT_like | 6.0e-15 | 855 | 1071 | 227 | + A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS. | ||
| cd00505 | Glyco_transf_8 | 3.0e-56 | 855 | 1102 | 257 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| pfam06427 | UDP-g_GGTase | 4.0e-78 | 528 | 733 | 212 | + UDP-glucose:Glycoprotein Glucosyltransferase. The N-terminal region of this group of proteins is required for correct folding of the ER UDP-Glc: glucosyltransferase. | ||
| cd06432 | GT8_HUGT1_C_like | 7.0e-180 | 855 | 1102 | 248 | + The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003980 | UDP-glucose:glycoprotein glucosyltransferase activity |
| GO:0006486 | protein glycosylation |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG51883.1 | 0 | 2 | 1162 | 457 | 1674 | AC016162_4 putative UDP-glucose:glycoprotein glucosyltransferase; 101200-91134 [Arabidopsis thaliana] |
| GenBank | EEE57527.1 | 0 | 2 | 1149 | 441 | 1588 | hypothetical protein OsJ_07839 [Oryza sativa Japonica Group] |
| RefSeq | NP_177278.3 | 0 | 2 | 1162 | 450 | 1613 | EBS1 (EMS-mutagenized bri1 suppressor 1); UDP-glucose:glycoprotein glucosyltransferase/ transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002268972.1 | 0 | 1 | 1162 | 453 | 1611 | PREDICTED: similar to EBS1 (EMS-MUTAGENIZED BRI1 SUPPRESSOR 1); UDP-glucose:glycoprotein glucosyltransferase/ transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Vitis vinifera] |
| RefSeq | XP_002529534.1 | 0 | 1 | 1052 | 456 | 1499 | UDP-glucose glycoprotein:glucosyltransferase, putative [Ricinus communis] |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| HO415354 | 585 | 599 | 1163 | 0 |
| HO797114 | 358 | 807 | 1163 | 0 |
| GO883503 | 321 | 819 | 1139 | 0 |
| DR944618 | 276 | 850 | 1125 | 0 |
| HO415354 | 151 | 451 | 600 | 2e-17 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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