| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g002111m |
| Family | GT35 |
| Protein Properties | Length: 966 Molecular Weight: 108774 Isoelectric Point: 4.8855 |
| Chromosome | Chromosome/Scaffold: 00015 Start: 1610108 End: 1617132 |
| Description | Glycosyl transferase, family 35 |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT35 | 176 | 959 | 0 |
| ALSKLGQSLENVVSQEPDAALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYGLFKQRITKDGQEEVAEDWLELGNPWEIERNDVSYPVKFYGKIVPGS DGKSHWIGGEDIKAVAYDIPIPGYKTKTTINLRLWSTMVPSEDFDLSAFNAGDHTKAAEALTNAEKICYILYPGDESVEGKVLRLKQQYTLCSASLQDII ARFEKRSGANVNWEEFPEKVAVQMNDTHPTLCIPELIRILIDLKGLSWKEAWNITQRTVAYTNHTVLPEALEKWSFELMQKLLPRHMEIIEMIDEELVHT IVSEYGTADPDLLEKRLKETRILENVDLPATFADLFVKTKESTDVVPDDELENCDEEGGPVDEELESAQEDGVLEEEKEAEAVQEPPQLVRMANLCVVGS HAVNGVAEIHSEIVTNEVFNEFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSSILTSWLGTEDWVTNTGKLAELRKFADNEDLQSQFRAAKRNNKMKVVSF IKEKTGYSVSPDAMFDIQVKRIHEYKRQLMNILGIVYRYKKMKEMSAVERKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEIGDLLKVIF VPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQEVGEENFFLFGARAHEIAGLRKERSEGKFVPDARFEEVKKFVK SGVFGSYNYDELMGSLEGNEGFGQADYFLVGKDFPSYLECQEKVDEAYCDQKRWTRMSIMNTAGSSKFSSDRTIQEYARDIWNI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 966 Download |
| MAVSQFSSMS TRPSEALVQC TSSLSRFIEF GSRNRTSKQK LLLIRTFNSR PPTTSFCIKC 60 VSSQPSPKTK DRVTEEDTSS SQNSSGPDTA SVASSIQYHA EFTPLFSPEK FEPPKAFFAT 120 AQSVRDSLII NWNSTYEYYE RLNVKQAYYL SMEFLQGRAL LNAIGNLGLT GAYAEALSKL 180 GQSLENVVSQ EPDAALGNGG LGRLASCFLD SMATLNYPAW GYGLRYKYGL FKQRITKDGQ 240 EEVAEDWLEL GNPWEIERND VSYPVKFYGK IVPGSDGKSH WIGGEDIKAV AYDIPIPGYK 300 TKTTINLRLW STMVPSEDFD LSAFNAGDHT KAAEALTNAE KICYILYPGD ESVEGKVLRL 360 KQQYTLCSAS LQDIIARFEK RSGANVNWEE FPEKVAVQMN DTHPTLCIPE LIRILIDLKG 420 LSWKEAWNIT QRTVAYTNHT VLPEALEKWS FELMQKLLPR HMEIIEMIDE ELVHTIVSEY 480 GTADPDLLEK RLKETRILEN VDLPATFADL FVKTKESTDV VPDDELENCD EEGGPVDEEL 540 ESAQEDGVLE EEKEAEAVQE PPQLVRMANL CVVGSHAVNG VAEIHSEIVT NEVFNEFYKL 600 WPEKFQNKTN GVTPRRWIRF CNPDLSSILT SWLGTEDWVT NTGKLAELRK FADNEDLQSQ 660 FRAAKRNNKM KVVSFIKEKT GYSVSPDAMF DIQVKRIHEY KRQLMNILGI VYRYKKMKEM 720 SAVERKAKFV PRVCIFGGKA FATYVQAKRI VKFITDVGAT VNHDPEIGDL LKVIFVPDYN 780 VSVAELLIPA SELSQHISTA GMEASGTSNM KFAMNGCILI GTLDGANVEI RQEVGEENFF 840 LFGARAHEIA GLRKERSEGK FVPDARFEEV KKFVKSGVFG SYNYDELMGS LEGNEGFGQA 900 DYFLVGKDFP SYLECQEKVD EAYCDQKRWT RMSIMNTAGS SKFSSDRTIQ EYARDIWNII 960 PVELP* 1020 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00343 | Phosphorylase | 0 | 176 | 961 | 791 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| COG0058 | GlgP | 0 | 87 | 959 | 878 | + Glucan phosphorylase [Carbohydrate transport and metabolism] | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 565 | 959 | 404 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 93 | 470 | 382 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| TIGR02093 | P_ylase | 0 | 565 | 959 | 400 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACJ11757.1 | 0 | 54 | 965 | 2 | 935 | alpha-1,4 glucan phosphorylase [Gossypium hirsutum] |
| EMBL | CBI22291.1 | 0 | 23 | 965 | 21 | 982 | unnamed protein product [Vitis vinifera] |
| Swiss-Prot | P53536 | 0 | 54 | 964 | 58 | 1002 | PHSL_VICFA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| RefSeq | XP_002279075.1 | 0 | 23 | 965 | 21 | 958 | PREDICTED: similar to Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic [Vitis vinifera] |
| RefSeq | XP_002526085.1 | 0 | 19 | 965 | 16 | 977 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ati_B | 0 | 88 | 964 | 23 | 832 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 2ati_A | 0 | 88 | 964 | 23 | 832 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 1fc0_B | 0 | 88 | 964 | 23 | 832 | A Chain A, Human Liver Glycogen Phosphorylase Complexed With N-Acetyl-Beta-D- Glucopyranosylamine |
| PDB | 1fc0_A | 0 | 88 | 964 | 23 | 832 | A Chain A, Human Liver Glycogen Phosphorylase Complexed With N-Acetyl-Beta-D- Glucopyranosylamine |
| PDB | 1fa9_A | 0 | 88 | 964 | 23 | 832 | A Chain A, Human Liver Glycogen Phosphorylase A Complexed With Amp |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO778303 | 871 | 116 | 966 | 0 |
| HO797178 | 401 | 566 | 966 | 0 |
| HO613954 | 514 | 453 | 966 | 0 |
| HO418036 | 587 | 302 | 885 | 0 |
| HO778303 | 63 | 58 | 118 | 0.0000003 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
