Basic Information | |
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Species | Citrus sinensis |
Cazyme ID | orange1.1g002256m |
Family | GH31 |
Protein Properties | Length: 947 Molecular Weight: 105419 Isoelectric Point: 7.11 |
Chromosome | Chromosome/Scaffold: 00019 Start: 308476 End: 313717 |
Description | alpha-xylosidase 1 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH31 | 293 | 785 | 0 |
FYFFAGPSPLAVVDQYTAFIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVENYKKAKIPLDVIWNDDDHMDGHKDFTLNPTNYPRPKLLAFLEKIHKIGM KYIVIIDPGIGVNSSYGVYQRGIANDVFIKYEGEPYLAQVWPGAVNFPDFLNPKTVSWWGDEIRRFHELVPVDGLWIDMNEASNFCSGLCKIPKGKQCPT GTGPGWVCCLDCKNITKTRWDDPPYKINASGLQVPIGFKTIATSAYHYNGVLEYDAHSIYGFSQSIATHKALLGLEGKRPFILSRSTFVGSGHYAAHWTG DNKGTWEDLKYSISTMLNFGIFGVPMVGSDICGFYPAPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQWESVAESARNALGMRYKLLPFLYTLNYEA HLSGAPIARPLFFSFPNYVECYNVSTQFLLGSSLMVSPVLEQGKSQVKALFPPGSWYNVFDMTQAISSKDGKFVTLDAPLHVVNVHLYQNTIL |
Full Sequence |
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Protein Sequence Length: 947 Download |
MCVYIYQIFQ RTHSLSSFSK QAKQMTSSLC FASFLLALLL CILSANSSST PPTKIGKGYR 60 LISIEEVDGG ILGHLQVKEK NNIYGPDIPL LQLYVKHETE DRLRVHITDA QKQRWEVPYN 120 LLPREQPPKL KQTIGRTRKN PIAVSDYSSN GLIFSYSADP FSFAVKRKSN GETLFNTSSD 180 ESDPFGPMVF KDQYLEISTK LPKDASLYGL GENTQPHGIK LYPNDPYTLY TTDVSAINLN 240 TDLYGSHPVY MDLRNVNGEG AAHGVLLLSS NGMDVFYKGT SLTYKIIGGV FDFYFFAGPS 300 PLAVVDQYTA FIGRPAPMPY WSLGFHQCRW GYHNLSVVED VVENYKKAKI PLDVIWNDDD 360 HMDGHKDFTL NPTNYPRPKL LAFLEKIHKI GMKYIVIIDP GIGVNSSYGV YQRGIANDVF 420 IKYEGEPYLA QVWPGAVNFP DFLNPKTVSW WGDEIRRFHE LVPVDGLWID MNEASNFCSG 480 LCKIPKGKQC PTGTGPGWVC CLDCKNITKT RWDDPPYKIN ASGLQVPIGF KTIATSAYHY 540 NGVLEYDAHS IYGFSQSIAT HKALLGLEGK RPFILSRSTF VGSGHYAAHW TGDNKGTWED 600 LKYSISTMLN FGIFGVPMVG SDICGFYPAP TEELCNRWIE VGAFYPFSRD HANYYSPRQE 660 LYQWESVAES ARNALGMRYK LLPFLYTLNY EAHLSGAPIA RPLFFSFPNY VECYNVSTQF 720 LLGSSLMVSP VLEQGKSQVK ALFPPGSWYN VFDMTQAISS KDGKFVTLDA PLHVVNVHLY 780 QNTILPMQQG GLISKEARMT PFSLVVTFPA GASGVQAKGK LYLDEDELPE MKLGNGYSTY 840 VDFFATTGNG TVKIWSEVQE GKFALSKGWI IDSVTVLGLG GSGKASTLEI NGSPTNANSK 900 IEFNASEQKH LNSVEDEQKS VMVGIKGLGF PVGKNFVMSW KMGISG* |
Functional Domains Download unfiltered results here | ||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
pfam01055 | Glyco_hydro_31 | 9.0e-87 | 294 | 480 | 187 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. |
cd06602 | GH31_MGAM_SI_GAA | 1.0e-88 | 313 | 477 | 169 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). |
cd06602 | GH31_MGAM_SI_GAA | 8.0e-93 | 546 | 714 | 170 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). |
pfam01055 | Glyco_hydro_31 | 2.0e-108 | 539 | 785 | 251 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. |
cd06604 | GH31_glucosidase_II_MalA | 1.0e-121 | 313 | 696 | 390 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAA10382.2 | 0 | 44 | 944 | 28 | 935 | alpha-D-xylosidase [Tropaeolum majus] |
RefSeq | XP_002282429.1 | 0 | 34 | 946 | 7 | 924 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
RefSeq | XP_002311455.1 | 0 | 44 | 946 | 5 | 910 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002315944.1 | 0 | 28 | 946 | 6 | 928 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002531635.1 | 0 | 25 | 946 | 2 | 930 | alpha-glucosidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 19 | 879 | 4 | 851 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
PDB | 3w37_A | 0 | 19 | 879 | 4 | 851 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
PDB | 3lpp_D | 0 | 58 | 940 | 80 | 898 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
PDB | 3lpp_C | 0 | 58 | 940 | 80 | 898 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
PDB | 3lpp_B | 0 | 58 | 940 | 80 | 898 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |