| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g002285m |
| Family | GT20 |
| Protein Properties | Length: 943 Molecular Weight: 106775 Isoelectric Point: 6.7218 |
| Chromosome | Chromosome/Scaffold: 00007 Start: 1135625 End: 1145270 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 102 | 571 | 0 |
| RCKQRLLVVANRLPVSAVRRGEDSWQLEISVGGLVSALLGVREFEARWIGWAGVNVPDEIGQKALTKALAEKRCIPVFLDEETVHQYYNGYCNNMLWPLF HYLGLPQEDRLATTRSFQSQFDAYKRANQMFAKVVNNIYEEGDVVWCHDYHLMFLPQCLKEYNNNMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADL VGFHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSDRFVRALELPQVQDHINELKERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLE ENPSWRDKVVLIQIAVPTRTDVPEYQKLTSQVHEIVGRINGRYGTLTTVPIHHLDRSLDFHALCALYAITDVALVTSLRDGMNLVSYEFVACQASKKGVL ILSEFAGAAQSLGAGAILVNPWNITEVASSIGYALNMPADEREKRHLHNFMHVTTHTSQEWAATFVSELN | |||
| Full Sequence |
|---|
| Protein Sequence Length: 943 Download |
| MNVNSFEEDL CVLPNNPPLL QTKRMPGNKY NSSPLTPRTR LERLLREREL RKNRSFLQNE 60 GEPDFFASES FLNASESWGP IDEEEFAGTR SSTDNGERLE GRCKQRLLVV ANRLPVSAVR 120 RGEDSWQLEI SVGGLVSALL GVREFEARWI GWAGVNVPDE IGQKALTKAL AEKRCIPVFL 180 DEETVHQYYN GYCNNMLWPL FHYLGLPQED RLATTRSFQS QFDAYKRANQ MFAKVVNNIY 240 EEGDVVWCHD YHLMFLPQCL KEYNNNMKVG WFLHTPFPSS EIHRTLPSRT ELLRSVLAAD 300 LVGFHTYDYA RHFVSACTRI LGLEGTPEGV EDQGRLTRVA AFPIGIDSDR FVRALELPQV 360 QDHINELKER FAGRKVMLGV DRLDMIKGIP QKILAFEKFL EENPSWRDKV VLIQIAVPTR 420 TDVPEYQKLT SQVHEIVGRI NGRYGTLTTV PIHHLDRSLD FHALCALYAI TDVALVTSLR 480 DGMNLVSYEF VACQASKKGV LILSEFAGAA QSLGAGAILV NPWNITEVAS SIGYALNMPA 540 DEREKRHLHN FMHVTTHTSQ EWAATFVSEL NDTIVEAQIR TRQVPPSLPI KGAVDSYLQS 600 NNRLLILGFN ATLTAPVDFL GRRGGQIREM EPKLHPDLKE PLKRLCDDPM TTVVVLSGSD 660 RNVLDDNFGE YNMWLAAENG MFLRLTTGEW MTTMPENLNM DWVDSVKHVF EYFTERTPRS 720 HFEVRETSLV WNYKYADLEF GRLQARDILQ HLWSGPISNA SVDVVQGGRS VEVRAVGVTK 780 GAAIDRILGE IVRHKGLKTP IDYVLCIGHF LQKDEDIYTF FEPELPFESP AGPRANAADH 840 LRRSSISQIP QAKSGPKVRF RKQRSLSSIE RKVSNYLTGG IWRPAMRDRM TLNEGSSVLD 900 LRGENYFSCT VSRKRSNARY FLGSSGDVVT LLNELAECPQ SN* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 25 | 937 | 932 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 104 | 936 | 833 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 106 | 572 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| PRK14501 | PRK14501 | 0 | 106 | 808 | 712 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| pfam00982 | Glyco_transf_20 | 0 | 105 | 572 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABO61742.1 | 0 | 25 | 942 | 1 | 923 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
| RefSeq | NP_177979.1 | 0 | 25 | 941 | 1 | 941 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002305707.1 | 0 | 45 | 942 | 1 | 899 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002331300.1 | 0 | 25 | 936 | 1 | 918 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002522296.1 | 0 | 25 | 937 | 1 | 910 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 106 | 582 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 106 | 582 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 106 | 582 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 106 | 582 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 0 | 106 | 574 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CX546763 | 311 | 88 | 395 | 0 |
| CV711307 | 293 | 104 | 396 | 0 |
| DW069910 | 288 | 192 | 479 | 0 |
| ES813375 | 360 | 467 | 826 | 0 |
| CX545499 | 305 | 409 | 713 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
