| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g002468m |
| Family | GT20 |
| Protein Properties | Length: 919 Molecular Weight: 103761 Isoelectric Point: 7.0488 |
| Chromosome | Chromosome/Scaffold: 00243 Start: 69382 End: 81695 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 105 | 541 | 0 |
| VSAIRRGVKEFEARWIGWAGVNVPDEIGQKALTKALAEKRCIPVFLDEDIVHQYYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQFAAYIKANQMFAD VVNKHYKDGDVVWCHDYHLMFLPKCLKEYNSDMKVGWFLHTPFPSSEIHRTLPSRSDLLRAVLAADLVGFHTYDYARHFVSACTRILGFEGTPEGVEDQG RLTRVAAFPIGIDSERFIRALEINPVQVHIKELQETFAGRKVMLGVDRLDMIKGIPQKLLAFEKFLEENSDWRGKVVLLQIAVPTRTDVPEYQRLTSQVH EIVGRINGRFGTLTAVPIHHLDRSLDFPALCALYAVTDVALVTSLRDGMNLVSYEFVACQDLKKGVLILSEFAGAAQSLGAGAILVNPWNITEVANAIAR ALNMSPEEREKRHWHNFTHVTTHTAQEWAETFVSELN | |||
| Full Sequence |
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| Protein Sequence Length: 919 Download |
| MPGNSYNSNS SNIPPDRVTR LVREKIERDL RKSSRASHPN DVTDNGGREV FEDEQRLRDG 60 DNLGPSIVDE DLEGPASTPN EGCERLDGRT FSRQRLLVVA NRLPVSAIRR GVKEFEARWI 120 GWAGVNVPDE IGQKALTKAL AEKRCIPVFL DEDIVHQYYN GYCNNILWPL FHYLGLPQED 180 RLATTRSFQS QFAAYIKANQ MFADVVNKHY KDGDVVWCHD YHLMFLPKCL KEYNSDMKVG 240 WFLHTPFPSS EIHRTLPSRS DLLRAVLAAD LVGFHTYDYA RHFVSACTRI LGFEGTPEGV 300 EDQGRLTRVA AFPIGIDSER FIRALEINPV QVHIKELQET FAGRKVMLGV DRLDMIKGIP 360 QKLLAFEKFL EENSDWRGKV VLLQIAVPTR TDVPEYQRLT SQVHEIVGRI NGRFGTLTAV 420 PIHHLDRSLD FPALCALYAV TDVALVTSLR DGMNLVSYEF VACQDLKKGV LILSEFAGAA 480 QSLGAGAILV NPWNITEVAN AIARALNMSP EEREKRHWHN FTHVTTHTAQ EWAETFVSEL 540 NDTVVEAQLR IKQVPPSLRE ADSIERYLRS NNRLLILGFN ATLTEPVDTP GRRGDQIREM 600 ELKLHPDLKQ PLNALCHDPK TTIVVLSGSD RNVLDKNFQE YNLWLAAENG MFLRCTTGKW 660 MTTMPEHLNM EWVDSLKHVF EYFTERTPRS HFEQRETSLV WNYKYADVEF GRIQARDMLQ 720 HLWTGPISNA SVEVVQGSKS VEVRAVGVTK GAAIDRILAE IVHSKKMKTA IDYVLCIGHF 780 LGKDEDVYAF FEPELPDDGP GIPRTRPTDA IKVPGERRQA LKVASRGSSK ISQGKTQRPA 840 QAPEKKTPNH NCTNVRQPSS DKTSYNVLDL NKENYFSCAV GRPRTNARFL LQSSDEVVSF 900 LKKLADASSS HEAHNGPT* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 1 | 910 | 935 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 93 | 910 | 837 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 95 | 542 | 464 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| PRK14501 | PRK14501 | 0 | 95 | 799 | 730 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABO61742.1 | 0 | 1 | 911 | 1 | 923 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
| RefSeq | NP_177979.1 | 0 | 1 | 910 | 1 | 941 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002285632.1 | 0 | 1 | 910 | 1 | 926 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002331300.1 | 0 | 1 | 909 | 1 | 922 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002531237.1 | 0 | 1 | 909 | 1 | 924 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 95 | 552 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 95 | 552 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 95 | 552 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 95 | 552 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1uqu_B | 0 | 95 | 552 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CX545499 | 305 | 379 | 683 | 0 |
| ES813375 | 376 | 437 | 811 | 0 |
| CX546763 | 313 | 72 | 365 | 0 |
| CV711468 | 276 | 378 | 653 | 0 |
| EY652507 | 265 | 609 | 873 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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