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Basic Information | |
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Species | Citrus sinensis |
Cazyme ID | orange1.1g002764m |
Family | GH38 |
Protein Properties | Length: 884 Molecular Weight: 100059 Isoelectric Point: 6.197 |
Chromosome | Chromosome/Scaffold: 00187 Start: 152163 End: 158371 |
Description | Glycosyl hydrolase family 38 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH38 | 1 | 222 | 0 |
MCMHDEAAPHYIDMIDQTTLGHRYIKEQFNVTPRIGWQIDPFGHSAVQAYLLSAEVGFDFLFFARIDYQDRAKRINEKTLEVVWRASKSLGSSAQIMTGI SPKNYEPPSDNFYFEVGDTSPIVQDDMNLFDYNVPERVNDFVSAAIAQANITRTNHIMWTMGTDFKYQYAHTWFRQMDKFIHYVNQDGRVNALYSTPSMY IDAKYAANESWPLKTDDFFPYA |
Full Sequence |
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Protein Sequence Length: 884 Download |
MCMHDEAAPH YIDMIDQTTL GHRYIKEQFN VTPRIGWQID PFGHSAVQAY LLSAEVGFDF 60 LFFARIDYQD RAKRINEKTL EVVWRASKSL GSSAQIMTGI SPKNYEPPSD NFYFEVGDTS 120 PIVQDDMNLF DYNVPERVND FVSAAIAQAN ITRTNHIMWT MGTDFKYQYA HTWFRQMDKF 180 IHYVNQDGRV NALYSTPSMY IDAKYAANES WPLKTDDFFP YADRENAYWT GYFTSRPALK 240 GYVRTMSAYY LAARQLEFFK GRNEVGLSTD SLADALALVQ HHDAVSGTEK QHVADDYAKR 300 LSIGYTEAEK VVAASLACLT EFSKNGCVNP ITNFQQCPLL NISYCPPSEV DLSSGKSLVV 360 VVYNPLGWKR EDIIRIPVIN ENVTVKDSKG KVIESQLLPL LKDSMSLRNS YSTAYLGKST 420 NVTPSYWLAF SASVPPLGFN TYIISSAKQA ASHSGMRTVY KSQIRQNDTI EVGPGNLKLL 480 YSGKRAKLMR YINSKTSVNK LLEQSYIYYR GDDGSKDLQP SGAYIFRPNG TYPIEPEGQL 540 SFTILRGPLL DEVHQRINPW IYQVTRVYKG KDHAEIEFTV GPIPIDDGIG KEVVTQITSN 600 MISNKTFYTD SSGRDFIERI RDYRKDWNLE VNQPIAGNYY PVNLGIYMKD NSSELSILVD 660 RSVGGSSIAD GQLELMLHRR LVADDNRGVS EALNETVCVF DKCEGLTIIG KYYLRIDPVG 720 EAAKWRRSFG QEIYSPFLLA FTEQDGDHWA NSHLATYSAM DTTYVLPDNV ALITLQELDS 780 GKILLRLAHL YEIEEDTDLS VMSSVELKKV FPKKKISKVT EMNLSANQER AVMEKKRLVW 840 RVEGSSKEQP TAVRGSPVDP AKLVVELAPM EIRTFVIEFY SER* 900 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam07748 | Glyco_hydro_38C | 3.0e-41 | 458 | 875 | 450 | + Glycosyl hydrolases family 38 C-terminal domain. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. | ||
pfam01074 | Glyco_hydro_38 | 5.0e-54 | 1 | 222 | 226 | + Glycosyl hydrolases family 38 N-terminal domain. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. | ||
cd00451 | GH38N_AMII_euk | 2.0e-54 | 1 | 185 | 185 | + N-terminal catalytic domain of eukaryotic class II alpha-mannosidases; glycoside hydrolase family 38 (GH38). The family corresponds to a group of eukaryotic class II alpha-mannosidases (AlphaMII), which contain Golgi alpha-mannosidases II (GMII), the major broad specificity lysosomal alpha-mannosidases (LAM, MAN2B1), the noval core-specific lysosomal alpha 1,6-mannosidases (Epman, MAN2B2), and similar proteins. GMII catalyzes the hydrolysis of the terminal both alpha-1,3-linked and alpha-1,6-linked mannoses from the high-mannose oligosaccharide GlcNAc(Man)5(GlcNAc)2 to yield GlcNAc(Man)3(GlcNAc)2 (GlcNAc, N-acetylglucosmine), which is the committed step of complex N-glycan synthesis. LAM is a broad specificity exoglycosidase hydrolyzing all known alpha 1,2-, alpha 1,3-, and alpha 1,6-mannosidic linkages from numerous high mannose type oligosaccharides. Different from LAM, Epman can efficiently cleave only the alpha 1,6-linked mannose residue from (Man)3GlcNAc, but not (Man)3(GlcNAc)2 or other larger high mannose oligosaccharides, in the core of N-linked glycans. Members in this family are retaining glycosyl hydrolases of family GH38 that employs a two-step mechanism involving the formation of a covalent glycosyl enzyme complex. Two carboxylic acids positioned within the active site act in concert: one as a catalytic nucleophile and the other as a general acid/base catalyst. | ||
PLN02701 | PLN02701 | 9.0e-70 | 3 | 691 | 779 | + alpha-mannosidase | ||
cd10810 | GH38N_AMII_LAM_like | 5.0e-102 | 2 | 186 | 187 | + N-terminal catalytic domain of lysosomal alpha-mannosidase and similar proteins; glycoside hydrolase family 38 (GH38). The subfamily is represented by lysosomal alpha-mannosidase (LAM, Man2B1, EC 3.2.1.114), which is a broad specificity exoglycosidase hydrolyzing all known alpha 1,2-, alpha 1,3-, and alpha 1,6-mannosidic linkages from numerous high mannose type oligosaccharides. LAM is expressed in all tissues and in many species. In mammals, the absence of LAM can cause the autosomal recessive disease alpha-mannosidosis. LAM has an acidic pH optimum at 4.0-4.5. It is stimulated by zinc ion and is inhibited by cobalt ion and plant alkaloids, such as swainsonine (SW). LAM catalyzes hydrolysis by a double displacement mechanism in which a glycosyl-enzyme intermediate is formed and hydrolyzed via oxacarbenium ion-like transition states. A carboxylic acid in the active site acts as the catalytic nucleophile in the formation of the covalent intermediate while a second carboxylic acid acts as a general acid catalyst. The same residue is thought to assist in the hydrolysis (deglycosylation) step, this time acting as a general base. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0004559 | alpha-mannosidase activity |
GO:0005975 | carbohydrate metabolic process |
GO:0006013 | mannose metabolic process |
GO:0008270 | zinc ion binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CBI15496.1 | 0 | 1 | 879 | 148 | 1041 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002277164.1 | 0 | 1 | 879 | 131 | 1019 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002280858.1 | 0 | 1 | 879 | 131 | 1008 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002318770.1 | 0 | 1 | 879 | 128 | 1011 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002511094.1 | 0 | 1 | 879 | 129 | 1012 | lysosomal alpha-mannosidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1ps3_A | 0 | 3 | 693 | 167 | 882 | A Chain A, Golgi Alpha-mannosidase Ii In Complex With Kifunensine |
PDB | 1hxk_A | 0 | 3 | 693 | 137 | 852 | A Chain A, Golgi Alpha-Mannosidase Ii In Complex With Deoxymannojirimicin |
PDB | 1hww_A | 0 | 3 | 693 | 137 | 852 | A Chain A, Golgi Alpha-Mannosidase Ii In Complex With Deoxymannojirimicin |
PDB | 1hty_A | 0 | 3 | 693 | 137 | 852 | A Chain A, Golgi Alpha-Mannosidase Ii |
PDB | 3eju_A | 0 | 3 | 693 | 167 | 882 | A Chain A, Golgi Alpha-Mannosidase Ii |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO780062 | 603 | 203 | 801 | 0 |
FG227394 | 493 | 58 | 548 | 0 |
DT755178 | 297 | 511 | 806 | 0 |
HO780062 | 87 | 118 | 204 | 2e-40 |
HO780062 | 24 | 282 | 305 | 0.01 |
Sequence Alignments (This image is cropped. Click for full image.) |
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