| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g003029m |
| Family | GT20 |
| Protein Properties | Length: 857 Molecular Weight: 97330.1 Isoelectric Point: 6.7284 |
| Chromosome | Chromosome/Scaffold: 00243 Start: 69382 End: 81695 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 92 | 560 | 0 |
| SRQRLLVVANRLPVSAIRRGEDSWSLEISAGGLVSALLGVKEFEARWIGWAGVNVPDEIGQKALTKALAEKRCIPVFLDEDIVHQYYNGYCNNILWPLFH YLGLPQEDRLATTRSFQSQFAAYIKANQMFADVVNKHYKDGDVVWCHDYHLMFLPKCLKEYNSDMKVGWFLHTPFPSSEIHRTLPSRSDLLRAVLAADLV GFHTYDYARHFVSACTRILGFEGTPEGVEDQGRLTRVAAFPIGIDSERFIRALEINPVQVHIKELQETFAGRKVMLGVDRLDMIKGIPQKLLAFEKFLEE NSDWRGKVVLLQIAVPTRTDVPEYQRLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFPALCALYAVTDVALVTSLRDGMNLVSYEFVACQDLKKGVLI LSEFAGAAQSLGAGAILVNPWNITEVANAIARALNMSPEEREKRHWHNFTHVTTHTAQEWAETFVSELN | |||
| Full Sequence |
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| Protein Sequence Length: 857 Download |
| MPGNSYNSNS SNIPPDRVTR LVREKIERDL RKSSRASHPN DVTDNGGREV FEDEQRLRDG 60 DNLGPSIVDE DLEGPASTPN EGCERLDGRT FSRQRLLVVA NRLPVSAIRR GEDSWSLEIS 120 AGGLVSALLG VKEFEARWIG WAGVNVPDEI GQKALTKALA EKRCIPVFLD EDIVHQYYNG 180 YCNNILWPLF HYLGLPQEDR LATTRSFQSQ FAAYIKANQM FADVVNKHYK DGDVVWCHDY 240 HLMFLPKCLK EYNSDMKVGW FLHTPFPSSE IHRTLPSRSD LLRAVLAADL VGFHTYDYAR 300 HFVSACTRIL GFEGTPEGVE DQGRLTRVAA FPIGIDSERF IRALEINPVQ VHIKELQETF 360 AGRKVMLGVD RLDMIKGIPQ KLLAFEKFLE ENSDWRGKVV LLQIAVPTRT DVPEYQRLTS 420 QVHEIVGRIN GRFGTLTAVP IHHLDRSLDF PALCALYAVT DVALVTSLRD GMNLVSYEFV 480 ACQDLKKGVL ILSEFAGAAQ SLGAGAILVN PWNITEVANA IARALNMSPE EREKRHWHNF 540 THVTTHTAQE WAETFVSELN DTVVEAQLRI KQVPPSLREA DSIERYLRSN NRLLILGFNA 600 TLTEPVDTPG RRGDQIREME LKLHPDLKQP LNALCHDPKT TIVVLSGSDR NVLDKNFQEY 660 NLWLAAENGM FLRCTTGKWM TTMPEHLNME WVDSLKHVFE YFTERTPRSH FEQRETSLVW 720 NYKYADVEFG RIQARDMLQH LWTGPISNAS VEVVQGSKSV EVRAVGVTKG AAIDRILAEI 780 VHSKKMKTAI DYVLCIGHFL GKNCNLPLQF LMQTISTVRL DSSFLHYLRM KMCMRFLSQN 840 YLMMARAFQE PDQQMQ* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 1 | 803 | 806 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 93 | 845 | 756 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 95 | 561 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 0 | 94 | 561 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 95 | 797 | 710 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABO61742.1 | 0 | 1 | 803 | 1 | 801 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
| EMBL | CAA69879.1 | 0 | 1 | 803 | 1 | 801 | trehalose-6-phosphate synthase [Arabidopsis thaliana] |
| RefSeq | NP_177979.1 | 0 | 1 | 803 | 1 | 801 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002331300.1 | 0 | 1 | 803 | 1 | 796 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002531237.1 | 0 | 1 | 803 | 1 | 799 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1uqu_B | 0 | 95 | 571 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| PDB | 1uqu_A | 0 | 95 | 571 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| PDB | 1uqt_B | 0 | 95 | 571 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| PDB | 1uqt_A | 0 | 95 | 571 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| PDB | 2wtx_D | 0 | 95 | 571 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CX545499 | 305 | 398 | 702 | 0 |
| CX546763 | 313 | 72 | 384 | 0 |
| CV711307 | 294 | 92 | 385 | 0 |
| ES813375 | 350 | 456 | 805 | 0 |
| CV711468 | 276 | 397 | 672 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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