| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g003055m |
| Family | GT35 |
| Protein Properties | Length: 853 Molecular Weight: 96012.5 Isoelectric Point: 4.4376 |
| Chromosome | Chromosome/Scaffold: 00015 Start: 1611105 End: 1617132 |
| Description | Glycosyl transferase, family 35 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
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| Family | Start | End | Evalue |
| GT35 | 25 | 351 | 0 |
| ALSKLGQSLENVVSQEPDAALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYGLFKQRITKDGQEEVAEDWLELGNPWEIERNDVSYPVKFYGKIVPGS DGKSHWIGGEDIKAVAYDIPIPGYKTKTTINLRLWSTMVPSEDFDLSAFNAGDHTKAAEALTNAEKVPICYILYPGDESVEGKVLRLKQQYTLCSASLQD IIARFEKRSGANVNWEEFPEKVAVQMNDTHPTLCIPELIRILIDLKGLSWKEAWNITQRTVAYTNHTVLPEALEKWSFELMQKLLPRHMEIIEMIDEELV HTIVSEYGTADPDLLEKRLKETRILEN | |||
| GT35 | 436 | 846 | 0 |
| LEEEKEAEAVQEPPQLVRMANLCVVGSHAVNGVAEIHSEIVTNEVFNEFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSSILTSWLGTEDWVTNTGKLAEL RKFADNEDLQSQFRAAKRNNKMKVVSFIKEKTGYSVSPDAMFDIQVKRIHEYKRQLMNILGIVYRYKKMKEMSAVERKAKFVPRVCIFGGKAFATYVQAK RIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQEVGEENFFLFGARAHE IAGLRKERSEGKFVPDARFEEVKKFVKSGVFGSYNYDELMGSLEGNEGFGQADYFLVGKDFPSYLECQEKVDEAYCDQKRWTRMSIMNTAGSSKFSSDRT IQEYARDIWNI | |||
| Full Sequence |
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| Protein Sequence Length: 853 Download |
| MEFLQGRALL NAIGNLGLTG AYAEALSKLG QSLENVVSQE PDAALGNGGL GRLASCFLDS 60 MATLNYPAWG YGLRYKYGLF KQRITKDGQE EVAEDWLELG NPWEIERNDV SYPVKFYGKI 120 VPGSDGKSHW IGGEDIKAVA YDIPIPGYKT KTTINLRLWS TMVPSEDFDL SAFNAGDHTK 180 AAEALTNAEK VPICYILYPG DESVEGKVLR LKQQYTLCSA SLQDIIARFE KRSGANVNWE 240 EFPEKVAVQM NDTHPTLCIP ELIRILIDLK GLSWKEAWNI TQRTVAYTNH TVLPEALEKW 300 SFELMQKLLP RHMEIIEMID EELVHTIVSE YGTADPDLLE KRLKETRILE NVDLPATFAD 360 LFVKTKESTD VVPDDELENC DEEGGPVDEE LESAQEDGVL EEESTDVVPD DELENCDEEG 420 GPVDEELESE QEDDVLEEEK EAEAVQEPPQ LVRMANLCVV GSHAVNGVAE IHSEIVTNEV 480 FNEFYKLWPE KFQNKTNGVT PRRWIRFCNP DLSSILTSWL GTEDWVTNTG KLAELRKFAD 540 NEDLQSQFRA AKRNNKMKVV SFIKEKTGYS VSPDAMFDIQ VKRIHEYKRQ LMNILGIVYR 600 YKKMKEMSAV ERKAKFVPRV CIFGGKAFAT YVQAKRIVKF ITDVGATVNH DPEIGDLLKV 660 IFVPDYNVSV AELLIPASEL SQHISTAGME ASGTSNMKFA MNGCILIGTL DGANVEIRQE 720 VGEENFFLFG ARAHEIAGLR KERSEGKFVP DARFEEVKKF VKSGVFGSYN YDELMGSLEG 780 NEGFGQADYF LVGKDFPSYL ECQEKVDEAY CDQKRWTRMS IMNTAGSSKF SSDRTIQEYA 840 RDIWNIIPVE LP* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00343 | Phosphorylase | 3.0e-138 | 25 | 350 | 326 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 8.0e-166 | 1 | 321 | 325 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 452 | 846 | 404 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| pfam00343 | Phosphorylase | 0 | 452 | 848 | 402 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| TIGR02093 | P_ylase | 0 | 452 | 846 | 400 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| Gene Ontology | |
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| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACJ11757.1 | 0 | 1 | 852 | 101 | 935 | alpha-1,4 glucan phosphorylase [Gossypium hirsutum] |
| Swiss-Prot | P27598 | 0 | 1 | 852 | 132 | 955 | PHSL_IPOBA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| Swiss-Prot | P53536 | 0 | 1 | 851 | 157 | 1002 | PHSL_VICFA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| RefSeq | XP_002305367.1 | 0 | 1 | 852 | 112 | 949 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002526085.1 | 0 | 1 | 852 | 149 | 977 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1qm5_B | 0 | 452 | 844 | 404 | 792 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 1qm5_B | 0 | 1 | 320 | 66 | 375 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 1qm5_A | 0 | 452 | 844 | 404 | 792 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 1qm5_A | 0 | 1 | 320 | 66 | 375 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 1e4o_B | 0 | 452 | 844 | 404 | 792 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| HO778303 | 853 | 1 | 853 | 0 |
| HO797178 | 401 | 453 | 853 | 0 |
| HO620767 | 403 | 451 | 853 | 0 |
| HO613954 | 403 | 451 | 853 | 0 |
| HO613954 | 58 | 304 | 361 | 0.00001 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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