| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g003508m |
| Family | GT20 |
| Protein Properties | Length: 815 Molecular Weight: 92200.1 Isoelectric Point: 7.0328 |
| Chromosome | Chromosome/Scaffold: 00243 Start: 69382 End: 80128 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 9 | 437 | 0 |
| KEFEARWIGWAGVNVPDEIGQKALTKALAEKRCIPVFLDEDIVHQYYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQFAAYIKANQMFADVVNKHYKD GDVVWCHDYHLMFLPKCLKEYNSDMKVGWFLHTPFPSSEIHRTLPSRSDLLRAVLAADLVGFHTYDYARHFVSACTRILGFEGTPEGVEDQGRLTRVAAF PIGIDSERFIRALEINPVQVHIKELQETFAGRKVMLGVDRLDMIKGIPQKLLAFEKFLEENSDWRGKVVLLQIAVPTRTDVPEYQRLTSQVHEIVGRING RFGTLTAVPIHHLDRSLDFPALCALYAVTDVALVTSLRDGMNLVSYEFVACQDLKKGVLILSEFAGAAQSLGAGAILVNPWNITEVANAIARALNMSPEE REKRHWHNFTHVTTHTAQEWAETFVSELN | |||
| Full Sequence |
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| Protein Sequence Length: 815 Download |
| MDKLFEGVKE FEARWIGWAG VNVPDEIGQK ALTKALAEKR CIPVFLDEDI VHQYYNGYCN 60 NILWPLFHYL GLPQEDRLAT TRSFQSQFAA YIKANQMFAD VVNKHYKDGD VVWCHDYHLM 120 FLPKCLKEYN SDMKVGWFLH TPFPSSEIHR TLPSRSDLLR AVLAADLVGF HTYDYARHFV 180 SACTRILGFE GTPEGVEDQG RLTRVAAFPI GIDSERFIRA LEINPVQVHI KELQETFAGR 240 KVMLGVDRLD MIKGIPQKLL AFEKFLEENS DWRGKVVLLQ IAVPTRTDVP EYQRLTSQVH 300 EIVGRINGRF GTLTAVPIHH LDRSLDFPAL CALYAVTDVA LVTSLRDGMN LVSYEFVACQ 360 DLKKGVLILS EFAGAAQSLG AGAILVNPWN ITEVANAIAR ALNMSPEERE KRHWHNFTHV 420 TTHTAQEWAE TFVSELNDTV VEAQLRIKQV PPSLREADSI ERYLRSNNRL LILGFNATLT 480 EPVDTPGRRG DQIREMELKL HPDLKQPLNA LCHDPKTTIV VLSGSDRNVL DKNFQEYNLW 540 LAAENGMFLR CTTGKWMTTM PEHLNMEWVD SLKHVFEYFT ERTPRSHFEQ RETSLVWNYK 600 YADVEFGRIQ ARDMLQHLWT GPISNASVEV VQGSKSVEVR AVGVTKGAAI DRILAEIVHS 660 KKMKTAIDYV LCIGHFLGKD EDVYAFFEPE LPDDGPGIPR TRPTDAIKVP GERRQALKVA 720 SRGSSKISQG KTQRPAQAPE KKTPNHNCTN VRQPSSDKTS YNVLDLNKEN YFSCAVGRPR 780 TNARFLLQSS DEVVSFLKKL ADASSSHEAH NGPT* 840 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 7 | 806 | 803 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 7 | 806 | 800 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 8 | 438 | 432 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 0 | 8 | 438 | 434 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 12 | 695 | 690 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABO61742.1 | 0 | 7 | 807 | 127 | 923 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
| RefSeq | NP_177979.1 | 0 | 7 | 806 | 128 | 941 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002285632.1 | 0 | 7 | 806 | 123 | 926 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002331300.1 | 0 | 7 | 805 | 123 | 922 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002531237.1 | 0 | 7 | 805 | 126 | 924 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 15 | 448 | 39 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 15 | 448 | 39 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 15 | 448 | 39 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 15 | 448 | 39 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1uqu_B | 0 | 15 | 448 | 39 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CX545499 | 305 | 275 | 579 | 0 |
| ES813375 | 376 | 333 | 707 | 0 |
| CV711468 | 276 | 274 | 549 | 0 |
| EY652507 | 265 | 505 | 769 | 0 |
| EY652507 | 22 | 777 | 798 | 0.23 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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