| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g003875m |
| Family | GT35 |
| Protein Properties | Length: 791 Molecular Weight: 89711.4 Isoelectric Point: 4.4577 |
| Chromosome | Chromosome/Scaffold: 00015 Start: 1611811 End: 1617132 |
| Description | Glycosyl transferase, family 35 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
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| Family | Start | End | Evalue |
| GT35 | 1 | 289 | 0 |
| MATLNYPAWGYGLRYKYGLFKQRITKDGQEEVAEDWLELGNPWEIERNDVSYPVKFYGKIVPGSDGKSHWIGGEDIKAVAYDIPIPGYKTKTTINLRLWS TMVPSEDFDLSAFNAGDHTKAAEALTNAEKICYILYPGDESVEGKVLRLKQQYTLCSASLQDIIARFEKRSGANVNWEEFPEKVAVQMNDTHPTLCIPEL IRILIDLKGLSWKEAWNITQRTVAYTNHTVLPEALEKWSFELMQKLLPRHMEIIEMIDEELVHTIVSEYGTADPDLLEKRLKETRILEN | |||
| GT35 | 374 | 784 | 0 |
| LEEEKEAEAVQEPPQLVRMANLCVVGSHAVNGVAEIHSEIVTNEVFNEFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSSILTSWLGTEDWVTNTGKLAEL RKFADNEDLQSQFRAAKRNNKMKVVSFIKEKTGYSVSPDAMFDIQVKRIHEYKRQLMNILGIVYRYKKMKEMSAVERKAKFVPRVCIFGGKAFATYVQAK RIVKFITDVGATVNHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQEVGEENFFLFGARAHE IAGLRKERSEGKFVPDARFEEVKKFVKSGVFGSYNYDELMGSLEGNEGFGQADYFLVGKDFPSYLECQEKVDEAYCDQKRWTRMSIMNTAGSSKFSSDRT IQEYARDIWNI | |||
| Full Sequence |
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| Protein Sequence Length: 791 Download |
| MATLNYPAWG YGLRYKYGLF KQRITKDGQE EVAEDWLELG NPWEIERNDV SYPVKFYGKI 60 VPGSDGKSHW IGGEDIKAVA YDIPIPGYKT KTTINLRLWS TMVPSEDFDL SAFNAGDHTK 120 AAEALTNAEK ICYILYPGDE SVEGKVLRLK QQYTLCSASL QDIIARFEKR SGANVNWEEF 180 PEKVAVQMND THPTLCIPEL IRILIDLKGL SWKEAWNITQ RTVAYTNHTV LPEALEKWSF 240 ELMQKLLPRH MEIIEMIDEE LVHTIVSEYG TADPDLLEKR LKETRILENV DLPATFADLF 300 VKTKESTDVV PDDELENCDE EGGPVDEELE SAQEDGVLEE ESTDVVPDDE LENCDEEGGP 360 VDEELESEQE DDVLEEEKEA EAVQEPPQLV RMANLCVVGS HAVNGVAEIH SEIVTNEVFN 420 EFYKLWPEKF QNKTNGVTPR RWIRFCNPDL SSILTSWLGT EDWVTNTGKL AELRKFADNE 480 DLQSQFRAAK RNNKMKVVSF IKEKTGYSVS PDAMFDIQVK RIHEYKRQLM NILGIVYRYK 540 KMKEMSAVER KAKFVPRVCI FGGKAFATYV QAKRIVKFIT DVGATVNHDP EIGDLLKVIF 600 VPDYNVSVAE LLIPASELSQ HISTAGMEAS GTSNMKFAMN GCILIGTLDG ANVEIRQEVG 660 EENFFLFGAR AHEIAGLRKE RSEGKFVPDA RFEEVKKFVK SGVFGSYNYD ELMGSLEGNE 720 GFGQADYFLV GKDFPSYLEC QEKVDEAYCD QKRWTRMSIM NTAGSSKFSS DRTIQEYARD 780 IWNIIPVELP * |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00343 | Phosphorylase | 2.0e-129 | 1 | 288 | 288 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 8.0e-141 | 1 | 259 | 263 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 390 | 784 | 404 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| pfam00343 | Phosphorylase | 0 | 390 | 786 | 402 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| TIGR02093 | P_ylase | 0 | 390 | 784 | 400 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| Gene Ontology | |
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| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACJ11757.1 | 0 | 1 | 790 | 161 | 935 | alpha-1,4 glucan phosphorylase [Gossypium hirsutum] |
| Swiss-Prot | P27598 | 0 | 1 | 790 | 192 | 955 | PHSL_IPOBA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| Swiss-Prot | P53536 | 0 | 1 | 789 | 217 | 1002 | PHSL_VICFA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| RefSeq | XP_002305367.1 | 0 | 1 | 790 | 172 | 949 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002526085.1 | 0 | 1 | 790 | 209 | 977 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2azd_B | 0 | 390 | 782 | 404 | 792 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 2azd_B | 0 | 1 | 258 | 126 | 375 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 2azd_A | 0 | 390 | 782 | 404 | 792 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 2azd_A | 0 | 1 | 258 | 126 | 375 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 2aw3_B | 0 | 390 | 782 | 404 | 792 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| HO778303 | 791 | 1 | 791 | 0 |
| HO797178 | 401 | 391 | 791 | 0 |
| HO620767 | 403 | 389 | 791 | 0 |
| HO613954 | 403 | 389 | 791 | 0 |
| HO613954 | 58 | 242 | 299 | 0.00001 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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