Basic Information | |
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Species | Citrus sinensis |
Cazyme ID | orange1.1g004353m |
Family | CBM45 |
Protein Properties | Length: 760 Molecular Weight: 85642.7 Isoelectric Point: 5.9931 |
Chromosome | Chromosome/Scaffold: 00003 Start: 2697619 End: 2704871 |
Description | alpha-amylase-like 3 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
CBM45 | 317 | 393 | 1.1e-23 |
VHWGVCRDDSKNWEIPAEPYPPETIVFKNKALRTLLQPKEGGKGCSRLFTVDEEFAGFLFVLKLNENTWLKCMENDF | |||
GH13 | 533 | 733 | 1.9e-26 |
ATELSSLGFSVIWLPPPTESVSPEGYMPRDLYNLSSRYGNIDELKDVVNKFHDVGMKILGDVVLNHRCAHYQNQNGVWNIFGGRLNWDDRAVVADDPHFQ GRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWLRNEIGYDGWRLDFVRGFWGGYVKDYLEATEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIIDWIN A | |||
CBM45 | 126 | 209 | 1.6e-27 |
LHWGVSFVGDNGSEWDQPPKKMRPPGSVSIKDYAIETPLKKLAEGDVFDQVNIDFDTRSDIAAINFVLKDEETGAWYQHRGRDF |
Full Sequence |
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Protein Sequence Length: 760 Download |
MSTVTIRPLL PSYRRANLNF RDRTNILLKP NYINYSIKSA PNARRFCSFK KLQKITVSSS 60 TSTSTSPATS TDTTPVRPGD VFFKETFPLK RTHAVEGKMF VRLQKGKDEK NWQLSVGCNI 120 PGKWILHWGV SFVGDNGSEW DQPPKKMRPP GSVSIKDYAI ETPLKKLAEG DVFDQVNIDF 180 DTRSDIAAIN FVLKDEETGA WYQHRGRDFK VPLVDYLQHD GNVIGTKSTF GLWPGALGQL 240 SKMILKADTS QSGIQDSSSE SCELKQENKH LEGFYEELPI VKEIIIENTV SVSVRKCPET 300 AKTLLNLETD LTGDVVVHWG VCRDDSKNWE IPAEPYPPET IVFKNKALRT LLQPKEGGKG 360 CSRLFTVDEE FAGFLFVLKL NENTWLKCME NDFYIPLTSS SCLPAESVQE MLIPGKAEEA 420 TQEVSQTAYT AGIIKEIRNL VSDFSSDISR KTKSKEAQKS ILLEIEKLAA EAYSIFRTTA 480 PTFFEEAAVE LEESKPPAKI SPGTGTGFEI LCQGFNWESH KSGRWYMELK EKATELSSLG 540 FSVIWLPPPT ESVSPEGYMP RDLYNLSSRY GNIDELKDVV NKFHDVGMKI LGDVVLNHRC 600 AHYQNQNGVW NIFGGRLNWD DRAVVADDPH FQGRGNKSSG DNFHAAPNID HSQDFVRKDI 660 KEWLCWLRNE IGYDGWRLDF VRGFWGGYVK DYLEATEPYF AVGEYWDSLS YTYGEMDHNQ 720 DAHRQRIIDW INAASGTAGA FDVTTKGILH SVSISVLHV* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PRK09441 | PRK09441 | 7.0e-38 | 509 | 743 | 303 | + cytoplasmic alpha-amylase; Reviewed | ||
PLN00196 | PLN00196 | 2.0e-73 | 509 | 750 | 254 | + alpha-amylase; Provisional | ||
PLN02361 | PLN02361 | 2.0e-93 | 507 | 749 | 246 | + alpha-amylase | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 1.0e-101 | 510 | 751 | 244 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02784 | PLN02784 | 0 | 1 | 752 | 753 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAX33231.1 | 0 | 1 | 752 | 1 | 753 | plastid alpha-amylase [Malus x domestica] |
EMBL | CAN69906.1 | 0 | 1 | 758 | 1 | 739 | hypothetical protein [Vitis vinifera] |
EMBL | CBI32016.1 | 0 | 1 | 752 | 1 | 737 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002270049.1 | 0 | 1 | 752 | 1 | 753 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002520134.1 | 0 | 1 | 752 | 1 | 753 | alpha-amylase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3bsg_A | 0 | 509 | 752 | 2 | 257 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 2qps_A | 0 | 509 | 752 | 2 | 257 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
PDB | 1rpk_A | 0 | 509 | 752 | 2 | 257 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
PDB | 1p6w_A | 0 | 509 | 752 | 2 | 257 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
PDB | 1ht6_A | 0 | 509 | 752 | 2 | 257 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EG631183 | 760 | 1 | 752 | 0 |
GR441435 | 291 | 455 | 745 | 0 |
ES805448 | 287 | 466 | 752 | 0 |
EY817287 | 271 | 476 | 746 | 0 |
EV551363 | 270 | 475 | 744 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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