| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g004485m |
| Family | GT35 |
| Protein Properties | Length: 750 Molecular Weight: 85043.2 Isoelectric Point: 7.0831 |
| Chromosome | Chromosome/Scaffold: 00026 Start: 391748 End: 397966 |
| Description | alpha-glucan phosphorylase 2 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT35 | 25 | 743 | 0 |
| ALNNLGHVLEEIAEQEKDAALGNGGLGRLASCFLDSMATLNLPAWGYGLRYRYGLFKQKITKQGQEEVAEDWLEKFSPWEVVRHDVVFPVRFFGSVMVNP NGTRKWVGGEVVQAVAYDIPIPGYKTKNTISLRLWDAKASAEDFNLFQFNDGQYESAAQLHSRAQQICAVLYPGDSTEEGKLLRLKQQFFLCSASLQDMI LRFKERKSGRQWSEFPSKVAVQLNDTHPTLAIPELMRLLMDEEGLGWDEAWDITTRTVAYTNHTVLPEALEKWSQAVMWKLLPRHMEIIEEIDKRFIAMV RSTRSDLESKIPSMCILDNNPKKPVVRMANLCVVSAHTVNGVAQLHSDILKADLFADYVSLWPNKLQNKTNGITPRRWLRFCNPELSKIITKWLKTDQWV TNLDLLVGLRQFADNTELQAEWESAKMASKKHLADYIWRVTGVTIDPNSLFDIQVKRIHEYKRQLLNILGAIYRYKKLKEMSPQERKKTTPRTIMIGGKA FATYTNAKRIVKLVNDVGEVVNTDPEVNSYLKVVFVPNYNVSVAELLIPGSELSQHISTAGMEASGTSNMKFSLNGCLIIGTLDGANVEIRQEIGEENFF LFGAVAEQVPKLRKEREDGLFKPDPRFEEAKQFIRSGAFGSYDYNPLLDSLEGNTGYGRGDYFLVGYDFPSYLEAQDRVDQAYKDQKKWLKMSILSTAGS GKFSSDRTIAQYAKEIWNI | |||
| Full Sequence |
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| Protein Sequence Length: 750 Download |
| MEFLQGRTLT NAIGSLDIQN AYADALNNLG HVLEEIAEQE KDAALGNGGL GRLASCFLDS 60 MATLNLPAWG YGLRYRYGLF KQKITKQGQE EVAEDWLEKF SPWEVVRHDV VFPVRFFGSV 120 MVNPNGTRKW VGGEVVQAVA YDIPIPGYKT KNTISLRLWD AKASAEDFNL FQFNDGQYES 180 AAQLHSRAQQ ICAVLYPGDS TEEGKLLRLK QQFFLCSASL QDMILRFKER KSGRQWSEFP 240 SKVAVQLNDT HPTLAIPELM RLLMDEEGLG WDEAWDITTR TVAYTNHTVL PEALEKWSQA 300 VMWKLLPRHM EIIEEIDKRF IAMVRSTRSD LESKIPSMCI LDNNPKKPVV RMANLCVVSA 360 HTVNGVAQLH SDILKADLFA DYVSLWPNKL QNKTNGITPR RWLRFCNPEL SKIITKWLKT 420 DQWVTNLDLL VGLRQFADNT ELQAEWESAK MASKKHLADY IWRVTGVTID PNSLFDIQVK 480 RIHEYKRQLL NILGAIYRYK KLKEMSPQER KKTTPRTIMI GGKAFATYTN AKRIVKLVND 540 VGEVVNTDPE VNSYLKVVFV PNYNVSVAEL LIPGSELSQH ISTAGMEASG TSNMKFSLNG 600 CLIIGTLDGA NVEIRQEIGE ENFFLFGAVA EQVPKLRKER EDGLFKPDPR FEEAKQFIRS 660 GAFGSYDYNP LLDSLEGNTG YGRGDYFLVG YDFPSYLEAQ DRVDQAYKDQ KKWLKMSILS 720 TAGSGKFSSD RTIAQYAKEI WNITECRTS* 780 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 1 | 743 | 751 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| TIGR02093 | P_ylase | 0 | 1 | 743 | 751 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| pfam00343 | Phosphorylase | 0 | 25 | 745 | 726 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| PRK14985 | PRK14985 | 0 | 1 | 741 | 747 | + maltodextrin phosphorylase; Provisional | ||
| COG0058 | GlgP | 0 | 1 | 743 | 747 | + Glucan phosphorylase [Carbohydrate transport and metabolism] | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM29154.1 | 0 | 1 | 749 | 92 | 840 | starch phosphorylase type H [Citrus hybrid cultivar] |
| Swiss-Prot | P32811 | 0 | 1 | 748 | 87 | 837 | PHSH_SOLTU RecName: Full=Alpha-glucan phosphorylase, H isozyme; AltName: Full=Starch phosphorylase H |
| RefSeq | XP_002280732.1 | 0 | 1 | 746 | 93 | 840 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002313399.1 | 0 | 1 | 748 | 102 | 852 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002520435.1 | 0 | 1 | 748 | 98 | 848 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1ygp_B | 0 | 1 | 743 | 104 | 875 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 1ygp_A | 0 | 1 | 743 | 104 | 875 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 1z8d_A | 0 | 1 | 747 | 88 | 832 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 4el5_A | 0 | 1 | 747 | 76 | 820 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 4el0_A | 0 | 1 | 747 | 76 | 820 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO782200 | 410 | 81 | 489 | 0 |
| HO418036 | 539 | 151 | 665 | 0 |
| HO586252 | 549 | 199 | 746 | 0 |
| HO613954 | 497 | 301 | 748 | 0 |
| EL450777 | 329 | 262 | 590 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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