| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g005402m |
| Family | GT35 |
| Protein Properties | Length: 699 Molecular Weight: 78439 Isoelectric Point: 4.55 |
| Chromosome | Chromosome/Scaffold: 00015 Start: 1611105 End: 1617132 |
| Description | Glycosyl transferase, family 35 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GT35 | 25 | 349 | 0 |
| ALSKLGQSLENVVSQEPDAALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYGLFKQRITKDGQEEVAEDWLELGNPWEIERNDVSYPVKFYGKIVPGS DGKSHWIGGEDIKAVAYDIPIPGYKTKTTINLRLWSTMVPSEDFDLSAFNAGDHTKAAEALTNAEKICYILYPGDESVEGKVLRLKQQYTLCSASLQDII ARFEKRSGANVNWEEFPEKVAVQMNDTHPTLCIPELIRILIDLKGLSWKEAWNITQRTVAYTNHTVLPEALEKWSFELMQKLLPRHMEIIEMIDEELVHT IVSEYGTADPDLLEKRLKETRILEN | |||
| GT35 | 400 | 692 | 0 |
| EESTDVVSFIKEKTGYSVSPDAMFDIQVKRIHEYKRQLMNILGIVYRYKKMKEMSAVERKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPE IGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQEVGEENFFLFGARAHEIAGLRKERSEGKFVPDAR FEEVKKFVKSGVFGSYNYDELMGSLEGNEGFGQADYFLVGKDFPSYLECQEKVDEAYCDQKRWTRMSIMNTAGSSKFSSDRTIQEYARDIWNI | |||
| Full Sequence |
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| Protein Sequence Length: 699 Download |
| MEFLQGRALL NAIGNLGLTG AYAEALSKLG QSLENVVSQE PDAALGNGGL GRLASCFLDS 60 MATLNYPAWG YGLRYKYGLF KQRITKDGQE EVAEDWLELG NPWEIERNDV SYPVKFYGKI 120 VPGSDGKSHW IGGEDIKAVA YDIPIPGYKT KTTINLRLWS TMVPSEDFDL SAFNAGDHTK 180 AAEALTNAEK ICYILYPGDE SVEGKVLRLK QQYTLCSASL QDIIARFEKR SGANVNWEEF 240 PEKVAVQMND THPTLCIPEL IRILIDLKGL SWKEAWNITQ RTVAYTNHTV LPEALEKWSF 300 ELMQKLLPRH MEIIEMIDEE LVHTIVSEYG TADPDLLEKR LKETRILENV DLPATFADLF 360 VKTKESTDVV PDDELENCDE EGGPVDEELE SAQEDGVLEE ESTDVVSFIK EKTGYSVSPD 420 AMFDIQVKRI HEYKRQLMNI LGIVYRYKKM KEMSAVERKA KFVPRVCIFG GKAFATYVQA 480 KRIVKFITDV GATVNHDPEI GDLLKVIFVP DYNVSVAELL IPASELSQHI STAGMEASGT 540 SNMKFAMNGC ILIGTLDGAN VEIRQEVGEE NFFLFGARAH EIAGLRKERS EGKFVPDARF 600 EEVKKFVKSG VFGSYNYDEL MGSLEGNEGF GQADYFLVGK DFPSYLECQE KVDEAYCDQK 660 RWTRMSIMNT AGSSKFSSDR TIQEYARDIW NIIPVELP* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00343 | Phosphorylase | 3.0e-141 | 408 | 694 | 292 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| pfam00343 | Phosphorylase | 3.0e-142 | 25 | 348 | 324 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 1.0e-151 | 407 | 692 | 295 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 1.0e-169 | 1 | 319 | 323 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| TIGR02093 | P_ylase | 0 | 1 | 692 | 765 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| Gene Ontology | |
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| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM29154.1 | 0 | 1 | 692 | 92 | 834 | starch phosphorylase type H [Citrus hybrid cultivar] |
| GenBank | EEC71819.1 | 0 | 1 | 692 | 89 | 835 | hypothetical protein OsI_04460 [Oryza sativa Indica Group] |
| RefSeq | NP_001044823.1 | 0 | 1 | 692 | 89 | 835 | Os01g0851700 [Oryza sativa (japonica cultivar-group)] |
| Swiss-Prot | Q9LKJ3 | 0 | 1 | 698 | 81 | 832 | PHSH_WHEAT RecName: Full=Alpha-glucan phosphorylase, H isozyme; AltName: Full=Starch phosphorylase H |
| RefSeq | XP_002313399.1 | 0 | 1 | 698 | 102 | 853 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ati_B | 0 | 1 | 697 | 87 | 832 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 2ati_A | 0 | 1 | 697 | 87 | 832 | A Chain A, Phosphorylase Recognition And Phosphorylysis Of Its Oligosaccharide Substrate: Answers To A Long Outstanding Question |
| PDB | 1fc0_B | 0 | 1 | 697 | 87 | 832 | A Chain A, Human Liver Glycogen Phosphorylase Complexed With N-Acetyl-Beta-D- Glucopyranosylamine |
| PDB | 1fc0_A | 0 | 1 | 697 | 87 | 832 | A Chain A, Human Liver Glycogen Phosphorylase Complexed With N-Acetyl-Beta-D- Glucopyranosylamine |
| PDB | 1fa9_A | 0 | 1 | 697 | 87 | 832 | A Chain A, Human Liver Glycogen Phosphorylase A Complexed With Amp |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| HO778303 | 374 | 1 | 374 | 0 |
| HO778303 | 295 | 405 | 699 | 0 |
| EY663438 | 286 | 412 | 697 | 0 |
| ES816557 | 337 | 38 | 373 | 0 |
| FE702466 | 285 | 408 | 692 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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