| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g008686m |
| Family | GH32 |
| Protein Properties | Length: 558 Molecular Weight: 62795.1 Isoelectric Point: 7.8181 |
| Chromosome | Chromosome/Scaffold: 00032 Start: 861786 End: 864246 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 53 | 366 | 0 |
| HFQPPSSWMNDPNGPMIYKGIYHFFYQYKLWDTVVWGHSTSTDMVNWTPHDAAIYPSQPFDANSCWSGSVTILPGGNPAVLYTGIDAGNKQVQNLAVPKN LSDPYLKEWIKPPENPLMVPADQIDPGSFRDPTTAWIGPDKIWRVIIGSKINDTGLAILYRSKDFVNWTKAEQSLYSTNQSGMWECPDFFPVSTKSPKGV DTSVIGPNIKHVLKVSLSNYQQDYYTIGTYNITTDTYIAEEGSFDDNSGLRYDYGKFYGSKTFFDSAKNRRILWGWINESSTRSDNLKKGWAGLQATPRN LWLDKSGRQLMQWP | |||
| Full Sequence |
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| Protein Sequence Length: 558 Download |
| MGYSSICLFI FFALFLGHVF VKLEASQHVY RTLQTCQSFS SSITSQPYRT GYHFQPPSSW 60 MNDPNGPMIY KGIYHFFYQY KLWDTVVWGH STSTDMVNWT PHDAAIYPSQ PFDANSCWSG 120 SVTILPGGNP AVLYTGIDAG NKQVQNLAVP KNLSDPYLKE WIKPPENPLM VPADQIDPGS 180 FRDPTTAWIG PDKIWRVIIG SKINDTGLAI LYRSKDFVNW TKAEQSLYST NQSGMWECPD 240 FFPVSTKSPK GVDTSVIGPN IKHVLKVSLS NYQQDYYTIG TYNITTDTYI AEEGSFDDNS 300 GLRYDYGKFY GSKTFFDSAK NRRILWGWIN ESSTRSDNLK KGWAGLQATP RNLWLDKSGR 360 QLMQWPISEI EKLRGNQVKW PAKLLERGSM VGITGITAAQ ADVEISFQTT AFEKAEVLDS 420 IWTNPQFLCP FGLLVLASNG LQEYTAVFFR IFKGQDRHIV LMCSDQSRSS INDDNDKTSY 480 GAFLDLDPVH EKLSIRSLID RSIVESFGGG GKACITARIY PTLAIHDKAH LYAFNNGTEK 540 VNITSLRAWS MKKAQIN* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 1.0e-50 | 45 | 521 | 500 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 1.0e-68 | 40 | 522 | 504 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 9.0e-85 | 60 | 369 | 326 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-131 | 53 | 366 | 326 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 1.0e-169 | 53 | 511 | 473 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAD10960.1 | 0 | 21 | 557 | 20 | 577 | cell wall invertase precursor [Fragaria x ananassa] |
| GenBank | AAT09980.1 | 0 | 8 | 557 | 9 | 576 | cell wall apoplastic invertase [Vitis vinifera] |
| EMBL | CAD91338.1 | 0 | 1 | 557 | 1 | 564 | beta-fructofuranosidase [Glycine max] |
| EMBL | CBI35961.1 | 0 | 17 | 557 | 18 | 574 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002279788.1 | 0 | 17 | 557 | 18 | 575 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 41 | 557 | 1 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2xqr_K | 0 | 45 | 557 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 45 | 557 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 45 | 557 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 45 | 557 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
