| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g010135m |
| Family | PL4 |
| Protein Properties | Length: 518 Molecular Weight: 59147.6 Isoelectric Point: 5.9581 |
| Chromosome | Chromosome/Scaffold: 00004 Start: 2590298 End: 2595149 |
| Description | Rhamnogalacturonate lyase family protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
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| Family | Start | End | Evalue |
| PL4 | 1 | 500 | 0 |
| MLRGSSGFYSYAIYEHLKEWPAFTLGQTRFTFKLRKEKFRYMVIADNRHRQMPLPEDLSTGRGQPLAYPEAVQLVNPTDPEHQGEVDDKYQYSCENKDLK VHGWICRTTPVGFWLIIPSDEFRSGGPLKQNLTSHVGPTTLAVFLSGHYAGKYMETHIGQDEPWKKVFGPVFIYLNSAADGDDPLWLWEDAKIKMMSEVQ SWPYNFPASEDFQKSEERGCVSGRLLVQDSNDVISANGAYVGLAPPGDVGSWQTECKDYQFWTTADEDGCFSIKNIRTGNYNLYAWVPGFVGDYRSDALV TITSGSNIKMGDLVYEPPRDGPTLWEIGIPDRSAREFNVPDPDPKYVNRLFVNHPDRFRQYGLWSRYTELYPNEDLVYTIGVSDYSKDWFFAQVVREMDN KTYQGTTWQIKFKLDHVDRNSSYKLRVAIASATLAELQVRVNDPNANRPLFTTGLIGRDNAIARHGIHGLYLLYHVNIPGTRFIEGENTIFLKQPRCTSP | |||
| Full Sequence |
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| Protein Sequence Length: 518 Download |
| MLRGSSGFYS YAIYEHLKEW PAFTLGQTRF TFKLRKEKFR YMVIADNRHR QMPLPEDLST 60 GRGQPLAYPE AVQLVNPTDP EHQGEVDDKY QYSCENKDLK VHGWICRTTP VGFWLIIPSD 120 EFRSGGPLKQ NLTSHVGPTT LAVFLSGHYA GKYMETHIGQ DEPWKKVFGP VFIYLNSAAD 180 GDDPLWLWED AKIKMMSEVQ SWPYNFPASE DFQKSEERGC VSGRLLVQDS NDVISANGAY 240 VGLAPPGDVG SWQTECKDYQ FWTTADEDGC FSIKNIRTGN YNLYAWVPGF VGDYRSDALV 300 TITSGSNIKM GDLVYEPPRD GPTLWEIGIP DRSAREFNVP DPDPKYVNRL FVNHPDRFRQ 360 YGLWSRYTEL YPNEDLVYTI GVSDYSKDWF FAQVVREMDN KTYQGTTWQI KFKLDHVDRN 420 SSYKLRVAIA SATLAELQVR VNDPNANRPL FTTGLIGRDN AIARHGIHGL YLLYHVNIPG 480 TRFIEGENTI FLKQPRCTSP FQGIMYDYIR LEGPPVS* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam13620 | CarboxypepD_reg | 0.0006 | 263 | 307 | 45 | + Carboxypeptidase regulatory-like domain. | ||
| cd10316 | RGL4_M | 9.0e-35 | 217 | 313 | 97 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| pfam06045 | Rhamnogal_lyase | 6.0e-38 | 1 | 86 | 86 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. | ||
| cd10320 | RGL4_N | 3.0e-53 | 1 | 178 | 178 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10317 | RGL4_C | 1.0e-53 | 325 | 512 | 190 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| Annotations - NR Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | XP_002285626.1 | 0 | 1 | 515 | 99 | 616 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
| RefSeq | XP_002301112.1 | 0 | 1 | 513 | 99 | 613 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002306520.1 | 0 | 1 | 515 | 99 | 616 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002527352.1 | 0 | 1 | 516 | 115 | 633 | lyase, putative [Ricinus communis] |
| RefSeq | XP_002527353.1 | 0 | 1 | 517 | 118 | 636 | lyase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1nkg_A | 0.008 | 260 | 493 | 283 | 484 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
| PDB | 3njx_A | 0.008 | 260 | 493 | 283 | 484 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
| PDB | 3njv_A | 0.008 | 260 | 493 | 283 | 484 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
| PDB | 2xhn_B | 0.008 | 260 | 493 | 283 | 484 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
| PDB | 2xhn_A | 0.008 | 260 | 493 | 283 | 484 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| EY731293 | 242 | 1 | 242 | 0 |
| EY792839 | 254 | 160 | 409 | 0 |
| EY842989 | 242 | 85 | 322 | 0 |
| EY780436 | 241 | 54 | 291 | 0 |
| EY842989 | 44 | 309 | 351 | 0.000007 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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