y
Basic Information | |
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Species | Citrus sinensis |
Cazyme ID | orange1.1g014892m |
Family | GH13 |
Protein Properties | Length: 417 Molecular Weight: 47674.5 Isoelectric Point: 8.4453 |
Chromosome | Chromosome/Scaffold: 00002 Start: 4249761 End: 4254937 |
Description | alpha-amylase-like 2 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 50 | 336 | 4.8e-31 |
RKVPDISKSGFTSVWLPPATHSFAPEGYLPQNLYSLNSSYGSEHLLKALLHKMKQHKVRAMADIVINHRVGTTQGHGGKYNRYDGIPLSWDEHAVTSCTG GLGNGSTGDNFHGVPNIDHTQHFVRKDIIAWLRWLRNTVGFQDFRFDFARGYSAKYVKEYIEGARPIFSVGEYWDSCNYNSHGLDYNQDSHRQRIINWID GTGQLSAAFDFTTKGILQEAVKGQFWRLRDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSNHIMEGYAYILMHPGIPSVFY |
Full Sequence |
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Protein Sequence Length: 417 Download |
MDYTSKGFDE TNQQTDLVKI RAVIRNGREI LFQGFNWESC KHDWWRNLER KVPDISKSGF 60 TSVWLPPATH SFAPEGYLPQ NLYSLNSSYG SEHLLKALLH KMKQHKVRAM ADIVINHRVG 120 TTQGHGGKYN RYDGIPLSWD EHAVTSCTGG LGNGSTGDNF HGVPNIDHTQ HFVRKDIIAW 180 LRWLRNTVGF QDFRFDFARG YSAKYVKEYI EGARPIFSVG EYWDSCNYNS HGLDYNQDSH 240 RQRIINWIDG TGQLSAAFDF TTKGILQEAV KGQFWRLRDA QGKPPGVMGW WPSRAVTFLD 300 NHDTGSTQAH WPFPSNHIME GYAYILMHPG IPSVFYDHFY DWGDSIHNQI VKLMDVRRQQ 360 DIHSRSSIKI LEAQSNLYSA IIGDKVCMKI GDGSWCPAGK EWTLATSGHK YAVWHK* 420 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PRK09441 | PRK09441 | 1.0e-37 | 26 | 359 | 417 | + cytoplasmic alpha-amylase; Reviewed | ||
PLN00196 | PLN00196 | 2.0e-138 | 29 | 416 | 407 | + alpha-amylase; Provisional | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 4.0e-161 | 30 | 368 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02784 | PLN02784 | 3.0e-161 | 9 | 416 | 415 | + alpha-amylase | ||
PLN02361 | PLN02361 | 0 | 22 | 416 | 397 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004556 | alpha-amylase activity |
GO:0005509 | calcium ion binding |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAF63239.1 | 0 | 1 | 416 | 1 | 413 | AF153828_1 alpha-amylase [Malus x domestica] |
GenBank | AAX33234.1 | 0 | 10 | 416 | 11 | 414 | cytosolic alpha-amylase [Malus x domestica] |
EMBL | CBI18895.1 | 0 | 1 | 416 | 1 | 411 | unnamed protein product [Vitis vinifera] |
RefSeq | NP_177740.1 | 0 | 1 | 416 | 1 | 413 | AMY2 (ALPHA-AMYLASE-LIKE 2); alpha-amylase/ calcium ion binding / catalytic/ cation binding [Arabidopsis thaliana] |
RefSeq | XP_002301935.1 | 0 | 12 | 416 | 5 | 406 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1bg9_A | 0 | 29 | 416 | 1 | 402 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
PDB | 1ava_B | 0 | 29 | 416 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
PDB | 1ava_A | 0 | 29 | 416 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
PDB | 1amy_A | 0 | 29 | 416 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
PDB | 3bsg_A | 0 | 29 | 416 | 2 | 404 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO780661 | 409 | 9 | 417 | 0 |
HO798064 | 369 | 22 | 390 | 0 |
BU103706 | 398 | 22 | 417 | 0 |
EY662127 | 286 | 67 | 352 | 0 |
HO798064 | 33 | 385 | 417 | 0.000000009 |
Sequence Alignments (This image is cropped. Click for full image.) |
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