| Basic Information | |
|---|---|
| Species | Citrus sinensis |
| Cazyme ID | orange1.1g017225m |
| Family | CE8 |
| Protein Properties | Length: 376 Molecular Weight: 40968.3 Isoelectric Point: 6.9452 |
| Chromosome | Chromosome/Scaffold: 00127 Start: 355064 End: 357281 |
| Description | pectin methylesterase PCR fragment F |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
 | |||
| Family | Start | End | Evalue |
| CE8 | 291 | 373 | 4.2e-22 |
| DIIVSKDGTGTVKTIAEAIKKAPENSDRRTIIYVRAGRYEESNLKVGRKKRNLMFIGDGKGKTIITGGRNVFDKLTTFHTASF | |||
| Full Sequence |
|---|
| Protein Sequence Length: 376 Download |
| MGYGRLQPSS DPGGSSFRED QPGHATSAPQ PSSRSRKKLI LLSVLSFALI IASAVSAVLL 60 VGLKTRAVGP TIGHKPTQAI SRTCSKTLYP NLCVSSLLNF PGSLTASEQD LVHISFNMTL 120 QHFSRALYFT SGISYLQMDP HVRSAFDDCL ELLDDSVDAL SRSLGSVLLL PGGDINKRED 180 VLTWLSAALT NHDTCSEGFE DLNGPVKDQV SDKLKDLSEL VSNCLAIFSA GGESDDFSGV 240 PIQNRRRRLL SSEEEEDDTW GENDDDDIAP RWVSRETRRL LSLPVPHIQA DIIVSKDGTG 300 TVKTIAEAIK KAPENSDRRT IIYVRAGRYE ESNLKVGRKK RNLMFIGDGK GKTIITGGRN 360 VFDKLTTFHT ASFGK* |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
 | ||||||||
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01614 | PME_inhib | 3.0e-39 | 75 | 228 | 154 | + pectinesterase inhibitor domain. This model describes a plant domain of about 200 amino acids, characterized by four conserved Cys residues, shown in a pectinesterase inhibitor from Kiwi to form two disulfide bonds: first to second and third to fourth. Roughly half the members of this family have the region described by This model followed immediately by a pectinesterase domain, pfam01095. This suggests that the pairing of the enzymatic domain and its inhibitor reflects a conserved regulatory mechanism for this enzyme family. | ||
| PLN02301 | PLN02301 | 7.0e-40 | 50 | 372 | 325 | + pectinesterase/pectinesterase inhibitor | ||
| PLN02314 | PLN02314 | 4.0e-40 | 49 | 373 | 349 | + pectinesterase | ||
| smart00856 | PMEI | 2.0e-43 | 75 | 227 | 153 | + Plant invertase/pectin methylesterase inhibitor. This domain inhibits pectin methylesterases (PMEs) and invertases through formation of a non-covalent 1:1 complex. It has been implicated in the regulation of fruit development, carbohydrate metabolism and cell wall extension. It may also be involved in inhibiting microbial pathogen PMEs. It has been observed that it is often expressed as a large inactive preprotein. It is also found at the N-termini of PMEs predicted from DNA sequences, suggesting that both PMEs and their inhibitors are expressed as a single polyprotein and subsequently processed. It has two disulphide bridges and is mainly alpha-helical. | ||
| PLN02484 | PLN02484 | 6.0e-161 | 1 | 373 | 378 | + probable pectinesterase/pectinesterase inhibitor | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004857 | enzyme inhibitor activity |
| GO:0005618 | cell wall |
| GO:0030599 | pectinesterase activity |
| GO:0042545 | cell wall modification |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAF42038.1 | 0 | 75 | 373 | 84 | 385 | pectin methylesterase 1 [Pyrus communis] |
| RefSeq | NP_200149.1 | 0 | 1 | 373 | 1 | 355 | PMEPCRF (PECTIN METHYLESTERASE PCR FRAGMENT F); pectinesterase [Arabidopsis thaliana] |
| RefSeq | XP_002278061.1 | 0 | 75 | 373 | 71 | 365 | PREDICTED: similar to pectin methylesterase 1 [Vitis vinifera] |
| RefSeq | XP_002317777.1 | 0 | 75 | 374 | 32 | 321 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002321999.1 | 0 | 1 | 374 | 1 | 374 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1gq8_A | 1e-17 | 291 | 372 | 8 | 88 | A Chain A, Pectin Methylesterase From Carrot |
| PDB | 1xg2_A | 2e-16 | 288 | 373 | 1 | 85 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 1xg2_B | 0.000004 | 80 | 221 | 6 | 143 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
