Basic Information | |
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Species | Citrus sinensis |
Cazyme ID | orange1.1g020504m |
Family | GH16 |
Protein Properties | Length: 326 Molecular Weight: 37008.5 Isoelectric Point: 6.7932 |
Chromosome | Chromosome/Scaffold: 00058 Start: 553290 End: 556101 |
Description | xyloglucan endotransglucosylase/hydrolase 28 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH16 | 42 | 206 | 7.6e-21 |
HDNLVVHTDGKSVHLSLDERTGSGFVSHDLYLHGFFSASIKLPADYTAGVVVAFYMSNGDMFEKNHDEIDFEFLGNIRGKDWRIQTNIYGNGSTSIGREE RYNLWFDPSDDFHQYSILWTDSQIMEFKRTASMGGDFPAKPMSLYATIWDGSDWATNGGKYRVNY |
Full Sequence |
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Protein Sequence Length: 326 Download |
MVVSYEGCFL LVFSLLAVVA SGLYRNLPIV PFDEGYSHLF GHDNLVVHTD GKSVHLSLDE 60 RTGSGFVSHD LYLHGFFSAS IKLPADYTAG VVVAFYMSNG DMFEKNHDEI DFEFLGNIRG 120 KDWRIQTNIY GNGSTSIGRE ERYNLWFDPS DDFHQYSILW TDSQIMEFKR TASMGGDFPA 180 KPMSLYATIW DGSDWATNGG KYRVNYKYAP YVTEFSDFVL HGCSFDPIEQ TSSKCDITES 240 SKVSIPTGVS PSQRIKMENF RRKHMTYSYC YDQIRYKVPP FECVINPLEA ERLKVHDPVT 300 FGGGRRHHGK RHHRSRSSGT KANDV* |
Functional Domains Download unfiltered results here | ||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
cd02175 | GH16_lichenase | 2.0e-20 | 46 | 202 | 175 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. |
cd00413 | Glyco_hydrolase_16 | 8.0e-21 | 35 | 200 | 180 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. |
PLN03161 | PLN03161 | 4.0e-46 | 40 | 283 | 264 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional |
pfam00722 | Glyco_hydro_16 | 5.0e-59 | 32 | 208 | 186 | + Glycosyl hydrolases family 16. |
cd02176 | GH16_XET | 9.0e-128 | 30 | 283 | 264 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005618 | cell wall |
GO:0005975 | carbohydrate metabolic process |
GO:0006073 | cellular glucan metabolic process |
GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABK92940.1 | 0 | 8 | 322 | 9 | 333 | unknown [Populus trichocarpa] |
GenBank | ABM91072.1 | 0 | 8 | 301 | 9 | 312 | xyloglucan endotransglycosylase/hydrolase precursor XTH-39 [Populus tremula] |
RefSeq | XP_002311545.1 | 0 | 8 | 322 | 9 | 333 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002315842.1 | 0 | 1 | 301 | 1 | 312 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002527547.1 | 0 | 1 | 322 | 1 | 335 | Xyloglucan endotransglucosylase/hydrolase protein 2 precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2uwc_B | 0 | 32 | 283 | 19 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
PDB | 2uwc_A | 0 | 32 | 283 | 19 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
PDB | 2uwa_C | 0 | 32 | 283 | 22 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
PDB | 2uwa_B | 0 | 32 | 283 | 22 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
PDB | 2uwa_A | 0 | 32 | 283 | 22 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |