Basic Information | |
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Species | Citrus sinensis |
Cazyme ID | orange1.1g028253m |
Family | GH16 |
Protein Properties | Length: 212 Molecular Weight: 24427.4 Isoelectric Point: 5.6175 |
Chromosome | Chromosome/Scaffold: 00006 Start: 383103 End: 384814 |
Description | xyloglucan endotransglucosylase/hydrolase 6 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH16 | 1 | 134 | 1.6e-21 |
MKIKLVPGDSAGTVTAFYMNSNTENVRDELDFEFLGNRTGQPYTVQTNIYANGKGDREQRVNLWFDPAADYHLYTILWNHHHIVFYVDDVPIRVYKNSGR APFPMNQPMGVYSTLWEADDWATRGGLEKIDWSK |
Full Sequence |
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Protein Sequence Length: 212 Download |
MKIKLVPGDS AGTVTAFYMN SNTENVRDEL DFEFLGNRTG QPYTVQTNIY ANGKGDREQR 60 VNLWFDPAAD YHLYTILWNH HHIVFYVDDV PIRVYKNSGR APFPMNQPMG VYSTLWEADD 120 WATRGGLEKI DWSKAPFYAY YRDFDIEGCP VPGPANCASN PGNWWEANNY QALTAMEARR 180 YRWVRLNHMI YDYCTDKSRY PVPPPECLAG I* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam06955 | XET_C | 4.0e-19 | 157 | 207 | 52 | + Xyloglucan endo-transglycosylase (XET) C-terminus. This family represents the C-terminus (approximately 60 residues) of plant xyloglucan endo-transglycosylase (XET). Xyloglucan is the predominant hemicellulose in the cell walls of most dicotyledons. With cellulose, it forms a network that strengthens the cell wall. XET catalyzes the splitting of xyloglucan chains and the linking of the newly generated reducing end to the non-reducing end of another xyloglucan chain, thereby loosening the cell wall. Note that all family members contain the pfam00722 domain. | ||
cd02183 | GH16_fungal_CRH1_transglycosylase | 2.0e-25 | 2 | 147 | 163 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
pfam00722 | Glyco_hydro_16 | 1.0e-60 | 1 | 134 | 135 | + Glycosyl hydrolases family 16. | ||
PLN03161 | PLN03161 | 1.0e-83 | 1 | 207 | 215 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
cd02176 | GH16_XET | 7.0e-135 | 1 | 207 | 211 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005618 | cell wall |
GO:0005975 | carbohydrate metabolic process |
GO:0006073 | cellular glucan metabolic process |
GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABB72442.1 | 0 | 1 | 211 | 85 | 297 | xyloglucan endotransglucosylase [Gerbera hybrid cultivar] |
GenBank | ABC55454.2 | 0 | 1 | 211 | 75 | 287 | xyloglucan endotransglucosylase/hydrolase 2 [Rosa x borboniana] |
GenBank | ABM91067.1 | 0 | 1 | 211 | 82 | 294 | xyloglucan endotransglycosylase/hydrolase precursor XTH-36 [Populus tremula x Populus tremuloides] |
GenBank | ABY86635.1 | 0 | 1 | 211 | 77 | 289 | xyloglucan endotransglycosylase/hydrolase [Gossypium raimondii] |
RefSeq | XP_002310324.1 | 0 | 1 | 211 | 84 | 296 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1un1_B | 0 | 1 | 207 | 64 | 272 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
PDB | 1un1_A | 0 | 1 | 207 | 64 | 272 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
PDB | 1umz_B | 0 | 1 | 207 | 64 | 272 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
PDB | 1umz_A | 0 | 1 | 207 | 64 | 272 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
PDB | 2uwb_B | 0 | 3 | 207 | 67 | 267 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EY856329 | 212 | 1 | 212 | 0 |
DC883501 | 212 | 1 | 212 | 0 |
DC884792 | 212 | 1 | 212 | 0 |
EY660164 | 212 | 1 | 212 | 0 |
EY827958 | 211 | 1 | 211 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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