| Basic Information | |
|---|---|
| Species | Prunus persica |
| Cazyme ID | ppa000429m |
| Family | CBM20 |
| Protein Properties | Length: 1192 Molecular Weight: 130674 Isoelectric Point: 6.6464 |
| Chromosome | Chromosome/Scaffold: 1 Start: 13769186 End: 13781547 |
| Description | catalytics;carbohydrate kinases;phosphoglucan, water dikinases |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM20 | 89 | 171 | 4.6e-21 |
| VRLNVRLDHQVEFGESVVILGSIKELGSWKKKVPMNWTESGWVCSLEFKGGESVEYKFLTVRADKTVLWEGGDNRVLKLPKGG | |||
| Full Sequence |
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| Protein Sequence Length: 1192 Download |
| MDCLHSLHTS ASSQLRRHKQ FRFLHHQHQC LGSLAVPPFF GHKRNFRPLC HQSNARLIVC 60 SAGVSSAQSI EEEKESKMKS KSKSGNEKVR LNVRLDHQVE FGESVVILGS IKELGSWKKK 120 VPMNWTESGW VCSLEFKGGE SVEYKFLTVR ADKTVLWEGG DNRVLKLPKG GNFGIVSHWN 180 ATGEAVDLLP LEKEEDVGNN GSTIVDTVST PEVGTSPFVG QWKGNAISFM RSNEHGNREA 240 GRILDTSGLQ GLALKLVEGD RNARNWWRKL EVVRDLLVGS SQSEDRLDAL INSAIYLKWI 300 NTGQIPCFED GGHHRPNRHA EISRVIFREL ERISCRKDTS PQEVLVVRKI HPCLPSFKAE 360 FTASVPLTRI RDIAHRNDIP HDLKQEIKHT IQNKLHRNAG PEDLVATEAM LARITKNPGE 420 YNEAFVEQFK IFHHELKDFF NAGSLAEQLE SIKDSIDDKG QSALALFLEC KKSLDTLEVS 480 NKGLGKNGTD LLFKTMKSLS DLREIIAKGL ESGLRNDAPD TAVAMRQKWR LCEIGLEDYS 540 FILLSRFLNE LDALGGAHWL AENVKSKDVS PWNDPLGALI VGIHQLRLSG WKPEECAAIE 600 NELLAWKARG LSEREGSEDG KIIWGLRHKA TLDRARRLTE EYSEALLQIF PQNVQILGKA 660 FGIPENSVRT YAEAEIRAGV IFQVSKLCTL LLKAVRTIIG SQGWDVIVPG AALGTLVQVE 720 RIVPGSIPST VEGPIVLMVN RADGDEEVTA AGSNIVGVIL LQELPHLSHL GVRARQEKVV 780 FVTCEDDDKV SDIQKHKGKY VRLEASPTSV DIYPSSENSN GSFAVKNLSG DAATKIEALG 840 THDPSQSPTK APYFQKGVSG GILLLADAEA ETSGAKAAAC GRLASLAAVS DKVYSDQGVP 900 ASFNVPVGAV IPFGSMELAL EQSKSTDLFL SFLDKIETLK PECGELDQLC SQLQELVSSL 960 QPPKDIINGI GRIFPGNARL IVRSSANVED LAGMSAAGLY DSIPNVSVSN PTVFANAISR 1020 VWASLYTRRA VLSRRSAGVP QKEATMAILV QEMLSPDLSF VLHTVSPTDQ DHNSVEAEIA 1080 SGLGETLASG TRGTPWRLSS GKFDGNVRTL AFANFSEELL GTGPADGEVI HLTVDYSKKP 1140 LTVDPIFRQQ LGQRLSTVGF FLEQKFGCPQ DIEGCVVGKD IYIVQTRPQP L* 1200 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01418 | PEP_synth | 1.0e-19 | 896 | 1189 | 313 | + phosphoenolpyruvate synthase. Also called pyruvate,water dikinase and PEP synthase. The member from Methanococcus jannaschii contains a large intein. This enzyme generates phosphoenolpyruvate (PEP) from pyruvate, hydrolyzing ATP to AMP and releasing inorganic phosphate in the process. The enzyme shows extensive homology to other enzymes that use PEP as substrate or product. This enzyme may provide PEP for gluconeogenesis, for PTS-type carbohydrate transport systems, or for other processes [Energy metabolism, Glycolysis/gluconeogenesis]. | ||
| cd05467 | CBM20 | 2.0e-20 | 89 | 179 | 97 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| PRK06241 | PRK06241 | 1.0e-38 | 901 | 1188 | 299 | + phosphoenolpyruvate synthase; Validated | ||
| cd05818 | CBM20_water_dikinase | 5.0e-48 | 89 | 179 | 91 | + Phosphoglucan water dikinase (also known as alpha-glucan water dikinase), N-terminal CBM20 (carbohydrate-binding module, family 20) domain. This domain is found in the chloroplast-encoded phosphoglucan water dikinase, one of two enzymes involved in the phosphorylation of plant starches. In addition to the CBM20 domain, phosphoglucan water dikinase contains a C-terminal pyruvate binding domain. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| pfam01326 | PPDK_N | 1.0e-52 | 901 | 1189 | 293 | + Pyruvate phosphate dikinase, PEP/pyruvate binding domain. This enzyme catalyzes the reversible conversion of ATP to AMP, pyrophosphate and phosphoenolpyruvate (PEP). | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005524 | ATP binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0016301 | kinase activity |
| GO:0016310 | phosphorylation |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACZ66259.1 | 0 | 1 | 1190 | 1 | 1201 | glucan/water dikinase [Solanum tuberosum] |
| EMBL | CBI39424.1 | 0 | 1 | 1190 | 1 | 1148 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_198009.3 | 0 | 42 | 1191 | 32 | 1196 | ATGWD3; carbohydrate kinase/ catalytic/ phosphoglucan, water dikinase [Arabidopsis thaliana] |
| RefSeq | XP_002265211.1 | 0 | 1 | 1190 | 1 | 1187 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002518612.1 | 0 | 1 | 1190 | 1 | 1174 | chloroplast alpha-glucan water dikinase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1kum_A | 0.0000007 | 89 | 170 | 7 | 95 | A Chain A, Pectin Methylesterase From Carrot |
| PDB | 1kul_A | 0.0000007 | 89 | 170 | 7 | 95 | A Chain A, Pectin Methylesterase From Carrot |
| PDB | 1acz_A | 0.0000007 | 89 | 170 | 7 | 95 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, 5 Structures |
| PDB | 1ac0_A | 0.0000007 | 89 | 170 | 7 | 95 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| PDB | 1pj9_A | 0.00002 | 102 | 179 | 601 | 684 | A Chain A, Bacillus Circulans Strain 251 Loop Mutant 183-195 |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO778000 | 883 | 321 | 1192 | 0 |
| HO794745 | 731 | 466 | 1192 | 0 |
| EL595870 | 437 | 588 | 1022 | 0 |
| EG657314 | 291 | 276 | 566 | 0 |
| HO794745 | 45 | 428 | 472 | 0.00000000008 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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