Basic Information | |
---|---|
Species | Prunus persica |
Cazyme ID | ppa000951m |
Family | GH13 |
Protein Properties | Length: 953 Molecular Weight: 107429 Isoelectric Point: 5.8393 |
Chromosome | Chromosome/Scaffold: 3 Start: 19058877 End: 19065497 |
Description | alpha-amylase-like 3 |
View CDS |
External Links |
---|
NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH13 | 584 | 868 | 2.7e-37 |
LSKIGVTSVWFPPPTESVAPQGYMPSDLYNLNSSYGSVEELKHCIGEMHSQGLLALGDVVLNHRCAQKQSPNGIWNIFGGKLAWGPEAIVCDDPNFQGCG NPSSGDIFHAAPNIDHSKDFVRNDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASVPAFAIGEYWDSLAYENGNLCYNQDAHRQRIVNWINATG GTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVLGWWPSRAVTFLENHDTGSTQGHWPFPRDKLTQGYAYILTHPGTPVIFYD |
Full Sequence |
---|
Protein Sequence Length: 953 Download |
MGSISLPDMA VGIIHHCPTA YSGAHWKYDK NLCLGRRPPI FRTASKRKKC DDSSDTFADV 60 VETSGRNEVL NIEEDELITA RKALSEAQAR QEAIEKERDQ LLEKLACSEA KQQEYVATIL 120 HEKELAIAEV EAAKSLFHQK LQESVEEKFS LESKLVLAKN DAVELAVQVE KLAEIAFQQA 180 TSHILEDAQL RVSAAETAAA EAAYEIEKQI RDVTEGSILS IVEQSKYAIE KALDVAEKAG 240 EHATKAVSEF TEGMNPLDEL ASIQSKNIML QGVVNDLESQ LLLTRSDVDR LKLELEKAHA 300 HANAFELRAK DAEKALLEFQ ESSKKNTLQK EEEIMSLIEK MKKDSSERKK ASSKAFKAEL 360 QSIRDAIGAA KEMAHSKDDA YLRRCEALQR SLKASEATTK MWRQRAEMAE SILCEERPLG 420 EGDEDSIYVV NGGRIDLLTD DDSQKWKLLS DGPRREIPQW MARKIRTIRP RFPPRKIDVA 480 EALSSKFRSL DLPKPNEVWS IAQEKPKEGD TLIEHVIEKE TIEKKRKALE HALQGKTIQW 540 QKTPEQTNLE SGTGTGREIV FQGFNWESWR KQWYLDLAPK AADLSKIGVT SVWFPPPTES 600 VAPQGYMPSD LYNLNSSYGS VEELKHCIGE MHSQGLLALG DVVLNHRCAQ KQSPNGIWNI 660 FGGKLAWGPE AIVCDDPNFQ GCGNPSSGDI FHAAPNIDHS KDFVRNDIKE WLNWLRNDIG 720 FDGWRLDFVR GFSGTYVKEY IEASVPAFAI GEYWDSLAYE NGNLCYNQDA HRQRIVNWIN 780 ATGGTSSAFD VTTKGILHSA LHNQYWRLID PQGKPTGVLG WWPSRAVTFL ENHDTGSTQG 840 HWPFPRDKLT QGYAYILTHP GTPVIFYDHL YDFGLHDILT ELIEARRRAG IHCRSAVKIY 900 HANNEGYVAQ IGDTLVMKLG HFDWNPSKEN HLEGSWQTFV DKGSDYKLWL RQ* 960 |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PRK09441 | PRK09441 | 2.0e-44 | 558 | 888 | 421 | + cytoplasmic alpha-amylase; Reviewed | ||
PLN00196 | PLN00196 | 3.0e-145 | 549 | 949 | 415 | + alpha-amylase; Provisional | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 3.0e-168 | 559 | 897 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02361 | PLN02361 | 3.0e-178 | 556 | 949 | 399 | + alpha-amylase | ||
PLN02784 | PLN02784 | 0 | 546 | 949 | 408 | + alpha-amylase |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004556 | alpha-amylase activity |
GO:0005509 | calcium ion binding |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CBI21221.1 | 0 | 1 | 952 | 1 | 975 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_001782830.1 | 0 | 560 | 951 | 14 | 404 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_002276872.1 | 0 | 1 | 945 | 1 | 968 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002324108.1 | 0 | 561 | 952 | 10 | 401 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002526120.1 | 0 | 112 | 952 | 136 | 972 | alpha-amylase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3bsg_A | 0 | 557 | 920 | 1 | 377 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 2qps_A | 0 | 557 | 920 | 1 | 377 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
PDB | 1rpk_A | 0 | 557 | 920 | 1 | 377 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
PDB | 1p6w_A | 0 | 557 | 920 | 1 | 377 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
PDB | 1ht6_A | 0 | 557 | 920 | 1 | 377 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
EST Download unfiltered results here | ||||
---|---|---|---|---|
Hit | Length | Start | End | EValue |
HO795567 | 639 | 315 | 953 | 0 |
HO778903 | 517 | 64 | 580 | 0 |
HO778903 | 105 | 580 | 684 | 0 |
HO785297 | 258 | 563 | 820 | 0 |
HO785297 | 103 | 840 | 942 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
---|