y
Basic Information | |
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Species | Prunus persica |
Cazyme ID | ppa001312m |
Family | GH13 |
Protein Properties | Length: 857 Molecular Weight: 97148.8 Isoelectric Point: 5.2425 |
Chromosome | Chromosome/Scaffold: 7 Start: 14466485 End: 14477964 |
Description | starch branching enzyme 2.2 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 366 | 686 | 4.4e-30 |
LPRIKRLGYNAVQLMAIQEHSYYASFGYHVTNFFAPSSRCGTPDDLKSLIDRAHELGILVLMDIVHSHASNNVLDGLNMFDGTDSHYFHSGSRGYHWMWD SRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLEVAFTGNYNEYFGLATDVDAVTYLMLVNDLIHGLYPEAVTIGEDVSGMPTFCVS VQDGGVGFDYRLHMAIADKWIELLQKIDEEWQMGDIVHTLTNRRWREKCVAYAESHDQALVGDKTIAFWLMDKDMYDFMALDRPATPLVDRGIALHKMIR LITMGLGGEGYLNFMGNEFGH |
Full Sequence |
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Protein Sequence Length: 857 Download |
MVSTLSGIRF PLLPSAYNNN NDSASLHSSF NGYRRTSSLS LFLTNSSFSR KIFAGKSSYD 60 SDSSLTVAAS KKVLVPDSQS DGSSSVTEQL EAPGTVSEDP QVLEDVDNVA MEDDKKVEDE 120 VKKSDVPSLD AGNVDGTEAK GEETPHPLDG TVSTAKKNAT QKSIPPPGNG KKIYEIDPLL 180 VGFRDHLDYR YGQYKRLREE IDKYEGGLEV FSRGYEKFGF TRSAEGITYR EWAPGAKSAS 240 LIGDFNNWNT NADVMTRNEF GVWEIFLPNN ADGSPPIPHG SRVKIRMDTP SGIKDSIPAW 300 IKFSVQAPGE IPYNGIYYDP PEEENYVFQH SQPKRPKSLR IYEAHVGMSS TEPKINTYAE 360 FRDDVLPRIK RLGYNAVQLM AIQEHSYYAS FGYHVTNFFA PSSRCGTPDD LKSLIDRAHE 420 LGILVLMDIV HSHASNNVLD GLNMFDGTDS HYFHSGSRGY HWMWDSRLFN YGSWEVLRYL 480 LSNARWWLEE YKFDGFRFDG VTSMMYTHHG LEVAFTGNYN EYFGLATDVD AVTYLMLVND 540 LIHGLYPEAV TIGEDVSGMP TFCVSVQDGG VGFDYRLHMA IADKWIELLQ KIDEEWQMGD 600 IVHTLTNRRW REKCVAYAES HDQALVGDKT IAFWLMDKDM YDFMALDRPA TPLVDRGIAL 660 HKMIRLITMG LGGEGYLNFM GNEFGHPEWI DFPRGTQQLP NGKIVPGNNN SFDKCRRRFD 720 LGDANYLRYH GLQEFDQAMQ HLEETYCFMT SEHQYISRKD EGDRVIVFER GNLVFVFNFH 780 WSKSYTDYRV GCLKPGKYKI VLDSDEKLFG GFNRIDHSAE YFTTDGWFDD RPHSFLLYAP 840 CRTAVVYALI AGPAKD* 900 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02960 | PLN02960 | 9.0e-10 | 183 | 267 | 91 | + alpha-amylase | ||
PLN03244 | PLN03244 | 3.0e-138 | 275 | 849 | 583 | + alpha-amylase; Provisional | ||
PLN02447 | PLN02447 | 0 | 134 | 850 | 721 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 0 | 322 | 737 | 417 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02960 | PLN02960 | 0 | 275 | 849 | 580 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABO31358.1 | 0 | 1 | 855 | 1 | 844 | starch branching enzyme II-1 [Malus x domestica] |
GenBank | ABO31359.1 | 0 | 1 | 855 | 1 | 844 | starch branching enzyme II-2 [Malus x domestica] |
EMBL | CAA56319.1 | 0 | 1 | 852 | 1 | 847 | starch branching enzyme I [Pisum sativum] |
EMBL | CBI30261.1 | 0 | 1 | 848 | 1 | 849 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002277213.1 | 0 | 41 | 848 | 244 | 1058 | PREDICTED: hypothetical protein [Vitis vinifera] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3amk_A | 0 | 173 | 847 | 13 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 173 | 847 | 13 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 173 | 847 | 13 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3aml_A | 0 | 173 | 847 | 13 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3k1d_A | 0 | 226 | 845 | 137 | 717 | A Chain A, Crystal Structure Of Glycogen Branching Enzyme Synonym: 1,4- Glucan:1,4-Alpha-D-Glucan 6-Glucosyl-Transferase From Mycob Tuberculosis H3 |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO794536 | 683 | 167 | 849 | 0 |
HO777638 | 627 | 223 | 849 | 0 |
HO458123 | 395 | 456 | 850 | 0 |
HO458123 | 296 | 167 | 455 | 0 |
HO777638 | 47 | 176 | 222 | 0.0000000002 |
Sequence Alignments (This image is cropped. Click for full image.) |
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