Basic Information | |
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Species | Prunus persica |
Cazyme ID | ppa002732m |
Family | GH32 |
Protein Properties | Length: 640 Molecular Weight: 70619.3 Isoelectric Point: 4.5233 |
Chromosome | Chromosome/Scaffold: 2 Start: 24263489 End: 24267701 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 116 | 434 | 0 |
HFQPERNWMNDPNGPMFYKGWYHLFYQYNPDSAVWGNITWGHAVSIDLIHWLYLPLAMVPDRWFDANGVWTGSSTILPDGQIVILYTGSTNESVQVQNLA YPANLSDPLLLHWVKYSGNPVLTAPPGIGSTDFRDPTTAWIGPDGLWRITLGSKVNKTGISIVYTTANFIDYELLEGVLHAVPGTGMWECVDFYPVSING SKGLETSANGPGVKHVLKASLDDKKMDYYAIGTYFVENNTWIPDDPKIDVGIGLKYDYGRYYASKTFYDQNKERRILLGWINETDTETDDLEKGWSSLQT IPRVVLFDNATGTNLLQWP |
Full Sequence |
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Protein Sequence Length: 640 Download |
MDTNTTSYTP LPGDPFLSGP PATPRRPLKG FAVIFAAVIF LMSLVALIIN QGPPQQDSHH 60 HKKQSASTAS SETTSYSRLP RGIAQGVSAK SNPSFSDEAS YNWTNAMFSW QRTAFHFQPE 120 RNWMNDPNGP MFYKGWYHLF YQYNPDSAVW GNITWGHAVS IDLIHWLYLP LAMVPDRWFD 180 ANGVWTGSST ILPDGQIVIL YTGSTNESVQ VQNLAYPANL SDPLLLHWVK YSGNPVLTAP 240 PGIGSTDFRD PTTAWIGPDG LWRITLGSKV NKTGISIVYT TANFIDYELL EGVLHAVPGT 300 GMWECVDFYP VSINGSKGLE TSANGPGVKH VLKASLDDKK MDYYAIGTYF VENNTWIPDD 360 PKIDVGIGLK YDYGRYYASK TFYDQNKERR ILLGWINETD TETDDLEKGW SSLQTIPRVV 420 LFDNATGTNL LQWPVEEIED LRLNTTEFSD VLVEAGTVVP LDIGTATQLD ILVDFEIQVL 480 ETECVNGNGS VGCGDGATDR STLGPFGILV LADETLSELT PVYFRVSNST DGDVTTYFCA 540 DETRSSKAPE VYKTVYGGQV PVLDGETYSA RVLVDHSIVE SFAQGGRTVI ASRIYPTEAI 600 YGAARLFLFN NATGVNVKAT LKIWELNSAF IHPFPLDQI* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 9.0e-54 | 112 | 596 | 504 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 1.0e-74 | 112 | 628 | 533 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-91 | 122 | 437 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 3.0e-150 | 116 | 434 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 116 | 586 | 481 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAL05427.2 | 0 | 1 | 639 | 1 | 636 | vacuolar acid invertase [Prunus cerasus] |
GenBank | ACQ82802.1 | 0 | 3 | 638 | 4 | 635 | vacuolar invertase [Gossypium hirsutum] |
DDBJ | BAF35858.1 | 0 | 3 | 639 | 4 | 645 | Soluble acid invertase [Pyrus pyrifolia var. culta] |
DDBJ | BAG30919.1 | 0 | 3 | 639 | 4 | 645 | soluble acid invertase [Pyrus pyrifolia] |
RefSeq | XP_002510944.1 | 0 | 9 | 638 | 15 | 638 | Beta-fructofuranosidase, soluble isoenzyme I precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 95 | 634 | 1 | 544 | I Chain I, Crystal Structure Of Skeletal Muscle Troponin In The Ca2+- Activated St |
PDB | 3ugh_A | 0 | 95 | 634 | 1 | 544 | I Chain I, Crystal Structure Of Skeletal Muscle Troponin In The Ca2+- Activated St |
PDB | 3ugg_B | 0 | 95 | 634 | 1 | 544 | I Chain I, Crystal Structure Of Skeletal Muscle Troponin In The Ca2+- Activated St |
PDB | 3ugg_A | 0 | 95 | 634 | 1 | 544 | I Chain I, Crystal Structure Of Skeletal Muscle Troponin In The Ca2+- Activated St |
PDB | 3ugf_B | 0 | 95 | 634 | 1 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |