Basic Information | |
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Species | Prunus persica |
Cazyme ID | ppa006152m |
Family | GH13 |
Protein Properties | Length: 426 Molecular Weight: 46875.7 Isoelectric Point: 5.1425 |
Chromosome | Chromosome/Scaffold: 8 Start: 14280622 End: 14282706 |
Description | alpha-amylase-like |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 35 | 343 | 2.1e-39 |
WKTEGGWYNSLRKSVPELASSGITHVWLPPPSQAASSEGYLPGRLYDLSASKYGSQDELKSLISAFHDSGIQAVADIVINHRTAEKQDERGIWCIFEGGT PDNRLDWGPSLICSDDTQYSDGKGNPDTGAGYDAAPDIDHKNTRVQKELSDWMNWLKTEIGFSGWRFDFVRGYSPEITKLYMTNTSPNFAVGELWNSLAI GSDGKPEYNQDAHRQELAGWVEGAGGAVTAFDFTTKGILQAAVQGELSRLKDPNGRAPGMIGLLPGKSVTFIDNHDTGSTQKIWPFPSDKVIQGYAYILT HPGIPSIFY |
Full Sequence |
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Protein Sequence Length: 426 Download |
MKSLTTLFCV LLCINLLPNF AASQILFQGF NWESWKTEGG WYNSLRKSVP ELASSGITHV 60 WLPPPSQAAS SEGYLPGRLY DLSASKYGSQ DELKSLISAF HDSGIQAVAD IVINHRTAEK 120 QDERGIWCIF EGGTPDNRLD WGPSLICSDD TQYSDGKGNP DTGAGYDAAP DIDHKNTRVQ 180 KELSDWMNWL KTEIGFSGWR FDFVRGYSPE ITKLYMTNTS PNFAVGELWN SLAIGSDGKP 240 EYNQDAHRQE LAGWVEGAGG AVTAFDFTTK GILQAAVQGE LSRLKDPNGR APGMIGLLPG 300 KSVTFIDNHD TGSTQKIWPF PSDKVIQGYA YILTHPGIPS IFYDHFFDWG LKDEITKLIA 360 IRSRNGIKPD SALRILASDA DLYVAAIDEK IIAKIGSRYD VGNLVPPTYQ VATSGKDYAV 420 WEKKA* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PRK09441 | PRK09441 | 6.0e-51 | 24 | 364 | 423 | + cytoplasmic alpha-amylase; Reviewed | ||
PLN02784 | PLN02784 | 1.0e-141 | 24 | 424 | 402 | + alpha-amylase | ||
PLN02361 | PLN02361 | 3.0e-151 | 24 | 423 | 405 | + alpha-amylase | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 3.0e-157 | 25 | 373 | 352 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN00196 | PLN00196 | 0 | 2 | 423 | 425 | + alpha-amylase; Provisional |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004556 | alpha-amylase activity |
GO:0005509 | calcium ion binding |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAX33232.1 | 0 | 28 | 425 | 7 | 394 | secreted alpha-amylase [Malus x domestica] |
DDBJ | BAC76729.1 | 0 | 1 | 422 | 1 | 421 | alpha-amylase [Vigna angularis] |
EMBL | CAN77038.1 | 0 | 4 | 424 | 3 | 442 | hypothetical protein [Vitis vinifera] |
RefSeq | XP_002282184.1 | 0 | 1 | 424 | 1 | 425 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002285213.1 | 0 | 4 | 424 | 3 | 424 | PREDICTED: hypothetical protein [Vitis vinifera] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1bg9_A | 0 | 24 | 423 | 1 | 402 | A Chain A, Arginine 116 Is Essential For Nucleic Acid Recognition By The Fingers Domain Of Moloney Murine Leukemia Virus Reverse Transcriptase |
PDB | 1ava_B | 0 | 24 | 423 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
PDB | 1ava_A | 0 | 24 | 423 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
PDB | 1amy_A | 0 | 24 | 423 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
PDB | 3bsg_A | 0 | 24 | 424 | 2 | 405 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |