Basic Information | |
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Species | Prunus persica |
Cazyme ID | ppa022745m |
Family | GH32 |
Protein Properties | Length: 469 Molecular Weight: 52694.7 Isoelectric Point: 8.7683 |
Chromosome | Chromosome/Scaffold: 7 Start: 13675499 End: 13678032 |
Description | cell wall invertase 4 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 56 | 249 | 1.7e-38 |
YHFQPPKHWINVSKTAWWHNGHWRILVGGKRKHRGMAWLYRSIDFKYWVKAKHPLHSAPETGMWECPDFFALALNGRSGVDTSKVGEDVKHILKVSLDET RYEYYTLGKYFPEKDRYVPDNTSVDGRAGLRLDYGNFYASKTFFDPSKNMRILWGWANESDAADDDKAKGWAGVQTIPRVVWLSSDAKQVVQWP |
Full Sequence |
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Protein Sequence Length: 469 Download |
ALIDTMFNKK LGLVFVFAAS VFLVINNNGV EASHNIYSRL QNAAAVEVNQ LHRTGYHFQP 60 PKHWINVSKT AWWHNGHWRI LVGGKRKHRG MAWLYRSIDF KYWVKAKHPL HSAPETGMWE 120 CPDFFALALN GRSGVDTSKV GEDVKHILKV SLDETRYEYY TLGKYFPEKD RYVPDNTSVD 180 GRAGLRLDYG NFYASKTFFD PSKNMRILWG WANESDAADD DKAKGWAGVQ TIPRVVWLSS 240 DAKQVVQWPI KELETLRGQK VDINNQNVEQ GQHVEVKGIT AAQADVEVTF SFGSLEKAEE 300 FDPNWANLDA QTVCSLRRSD VPGGVGPFGL LTLASQNLEE FTPIFFRVFK TKETKHKVLM 360 CSDAKSSSLR PFNEKLYRPS FAGFVDVDLS ADKKISLRSL IDHSVVESFG AGGKTCITSR 420 VYPTLAVNDA AHLFVFNNGT KPVTVQTLSA WSVNAPQQMN QNLNSEHI* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd08996 | GH32_B_Fructosidase | 2.0e-30 | 72 | 252 | 190 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-53 | 69 | 249 | 190 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 2.0e-88 | 69 | 413 | 352 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
Swiss-Prot | P26792 | 0.000005 | 28 | 93 | 35 | 98 | INV1_DAUCA RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 1; AltName: Full=Sucrose hydrolase 1; AltName: Full=Invertase 1; AltName: Full=Cell wall beta-fructosidase 1; Flags: Precursor |
Swiss-Prot | P26792 | 0 | 70 | 460 | 204 | 592 | INV1_DAUCA RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 1; AltName: Full=Sucrose hydrolase 1; AltName: Full=Invertase 1; AltName: Full=Cell wall beta-fructosidase 1; Flags: Precursor |
RefSeq | XP_002282171.1 | 0 | 70 | 457 | 123 | 503 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
RefSeq | XP_002530337.1 | 0.00000007 | 16 | 93 | 12 | 86 | Beta-fructofuranosidase, insoluble isoenzyme 3 precursor, putative [Ricinus communis] |
RefSeq | XP_002530337.1 | 0 | 70 | 456 | 189 | 570 | Beta-fructofuranosidase, insoluble isoenzyme 3 precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2qqw_A | 0 | 70 | 457 | 148 | 535 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
PDB | 2xqr_K | 0 | 70 | 457 | 148 | 535 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2xqr_I | 0 | 70 | 457 | 148 | 535 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
CT842376 | 391 | 70 | 456 | 0 |
EX274575 | 295 | 99 | 393 | 0 |
GO511990 | 232 | 191 | 421 | 0 |
DY291352 | 283 | 70 | 348 | 0 |
CT842376 | 67 | 33 | 93 | 9.5 |
Sequence Alignments (This image is cropped. Click for full image.) |
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