Basic Information | |
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Species | Oryza sativa |
Cazyme ID | LOC_Os02g12780.1 |
Family | AA7 |
Protein Properties | Length: 525 Molecular Weight: 56958.1 Isoelectric Point: 6.9398 |
Chromosome | Chromosome/Scaffold: 2 Start: 6693857 End: 6695685 |
Description | cytokinin oxidase 5 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
AA7 | 53 | 245 | 3.1e-30 |
RIVAAAPEAVLHPATPAEIAELVRFSASSPSPFPVAPRGQGHSARGQSLAPGGVVVDMRALASRRGRVNVSAGAAPYVDAGGEQLWADVLRATLEHGLAP RVWTDYLRITVAGTLSNAGIGGQAFRHGPQIANVLELDVITGTGDMVTCSRDKDSDLFFAVLGGLGQFGIITRARIGLMPAPKRVRWVRLAYS |
Full Sequence |
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Protein Sequence Length: 525 Download |
MAARCSIAFM IMASCLSVVV SGGLPGDLFA LSVASKLRVD RNSTARASSD FGRIVAAAPE 60 AVLHPATPAE IAELVRFSAS SPSPFPVAPR GQGHSARGQS LAPGGVVVDM RALASRRGRV 120 NVSAGAAPYV DAGGEQLWAD VLRATLEHGL APRVWTDYLR ITVAGTLSNA GIGGQAFRHG 180 PQIANVLELD VITGTGDMVT CSRDKDSDLF FAVLGGLGQF GIITRARIGL MPAPKRVRWV 240 RLAYSDVATF TKDQELLISK RASEAGFDYV EGQVQLNRTL TEGPKSTPFF SSSDIGRLAG 300 LASKSVSGVI YVIEGTMYYN ESTSTTMDQK LESILGQLSF EEGFVFTKDV RYVQFLDRVR 360 EEERVLRSIG MWDVPHPWLN LFVPRSRILD FDAGVFKGVF AGANPVGVIL MYPMNTNMWD 420 DCMMAVASDD DVFYAVGLLR SAAVIGDVER LEKENEAVLA FCHNEDIGCK QYLPYYTSQD 480 GWQRHFGAKW SRVADLKAKY DPHRILSPGQ RIFSSPASMV VVSM* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01678 | FAD_lactone_ox | 9.0e-9 | 49 | 251 | 208 | + sugar 1,4-lactone oxidases. This model represents a family of at least two different sugar 1,4 lactone oxidases, both involved in synthesizing ascorbic acid or a derivative. These include L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. Members are proposed to have the cofactor FAD covalently bound at a site specified by Prosite motif PS00862; OX2_COVAL_FAD; 1. | ||
pfam01565 | FAD_binding_4 | 4.0e-18 | 59 | 202 | 145 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
COG0277 | GlcD | 9.0e-25 | 34 | 510 | 491 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
pfam09265 | Cytokin-bind | 4.0e-157 | 234 | 513 | 283 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
PLN02441 | PLN02441 | 0 | 1 | 516 | 525 | + cytokinin dehydrogenase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005576 | extracellular region |
GO:0008152 | metabolic process |
GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
GO:0009056 | catabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAD17196.1 | 0 | 1 | 524 | 1 | 527 | putative cytokinin dehydrogenase 1 precursor [Oryza sativa Japonica Group] |
DDBJ | BAD17197.1 | 0 | 1 | 524 | 1 | 524 | putative cytokinin dehydrogenase 1 precursor [Oryza sativa Japonica Group] |
GenBank | EAY85042.1 | 0 | 1 | 524 | 1 | 524 | hypothetical protein OsI_06399 [Oryza sativa Indica Group] |
GenBank | EAZ22265.1 | 0 | 1 | 524 | 1 | 508 | hypothetical protein OsJ_05920 [Oryza sativa Japonica Group] |
GenBank | EEC72755.1 | 0 | 1 | 524 | 1 | 527 | hypothetical protein OsI_06398 [Oryza sativa Indica Group] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3s1e_A | 0 | 25 | 514 | 17 | 516 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
PDB | 3s1c_A | 0 | 25 | 514 | 17 | 516 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
PDB | 3dq0_A | 0 | 25 | 514 | 17 | 516 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
PDB | 3c0p_A | 0 | 25 | 514 | 17 | 516 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
PDB | 3bw7_A | 0 | 25 | 514 | 17 | 516 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
cytokinins degradation | RXN-4621 | EC-1.5.99 | isopentenyladenine:FAD oxidoreductase |
cytokinins degradation | RXN-4641 | EC-1.5.99 | isopentenyl adenosine:FAD oxidoreductase |
cytokinins degradation | RXN-4661 | EC-1.5.99 | trans-zeatin:FAD oxidoreductase |
cytokinins degradation | RXN-4662 | EC-1.5.99 | cis-zeatin:FAD oxidoreductase |
cytokinins degradation | RXN-4681 | EC-1.5.99 | trans-zeatin riboside:FAD oxidoreductase |