| Basic Information | |
|---|---|
| Species | Setaria italica |
| Cazyme ID | Si012051m |
| Family | AA7 |
| Protein Properties | Length: 415 Molecular Weight: 44212.4 Isoelectric Point: 5.2417 |
| Chromosome | Chromosome/Scaffold: 7 Start: 25332694 End: 25334400 |
| Description | cytokinin oxidase/dehydrogenase 1 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| AA7 | 50 | 245 | 2e-29 |
| VVSEAPVAVMRPGSAADVARLLGALSSAPAGPGRRPRASVAARGAGHSLHGQAQARGGIVVETRALPRAVEVGGGGAYADVGAGALWVEVLEECLRAGLA PRSWTDYLYLTVGGTLSNGGISGQAFKHGPQISNVLQLEVVTGTGEVVTCSPTQSPELFFAVLGGLGQFGIITRARIPLQVAPPKVRWVRAFYDSF | |||
| Full Sequence |
|---|
| Protein Sequence Length: 415 Download |
| MVLKALYLYA AVVVLLLCSS ANFIQSPSDV FGPVALLEPT PSAARDFGAV VSEAPVAVMR 60 PGSAADVARL LGALSSAPAG PGRRPRASVA ARGAGHSLHG QAQARGGIVV ETRALPRAVE 120 VGGGGAYADV GAGALWVEVL EECLRAGLAP RSWTDYLYLT VGGTLSNGGI SGQAFKHGPQ 180 ISNVLQLEVV TGTGEVVTCS PTQSPELFFA VLGGLGQFGI ITRARIPLQV APPKVRWVRA 240 FYDSFETFTG DQELLVSMPE LVDYVEGFMV LNEQSLRSSS VAFPAEVNFT PDFGSDGVGG 300 GKKVYYCIEF AVHDFQRQDS AAAADGDHVV ELVSGKLSFL RPHAYSVEVA YFDFLNRVRM 360 EEESLRSRGL WDVPHPWLNV FVPRHGAAAF KDLLMGTVTR GEFEGPVLVY PLLTD 420 |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam01565 | FAD_binding_4 | 3.0e-14 | 55 | 187 | 134 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| COG0277 | GlcD | 1.0e-16 | 54 | 247 | 200 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam09265 | Cytokin-bind | 2.0e-74 | 232 | 415 | 190 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 3.0e-179 | 18 | 415 | 420 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAE05712.2 | 0 | 1 | 415 | 1 | 415 | OSJNBb0065J09.8 [Oryza sativa (japonica cultivar-group)] |
| GenBank | EAY82296.1 | 0 | 22 | 413 | 24 | 406 | hypothetical protein OsI_37507 [Oryza sativa Indica Group] |
| GenBank | EAZ19670.1 | 0 | 22 | 413 | 24 | 406 | hypothetical protein OsJ_35246 [Oryza sativa Japonica Group] |
| RefSeq | XP_002446804.1 | 0 | 4 | 415 | 5 | 418 | hypothetical protein SORBIDRAFT_06g022930 [Sorghum bicolor] |
| RefSeq | XP_002467062.1 | 0 | 18 | 415 | 20 | 408 | hypothetical protein SORBIDRAFT_01g019000 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2q4w_A | 0 | 43 | 413 | 50 | 418 | A Chain A, The Crystal Structure Of Rice (Oryza Sativa L.) Os4bglu12 |
| PDB | 2exr_A | 0 | 43 | 413 | 50 | 418 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 3s1c_A | 0 | 40 | 412 | 36 | 415 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 3dq0_A | 0 | 40 | 412 | 36 | 415 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 3c0p_A | 0 | 40 | 412 | 36 | 415 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
