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CAZyme Information: MGYG000001224_00390

You are here: Home > Sequence: MGYG000001224_00390

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Blautia_A sp900547615
Lineage Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia_A; Blautia_A sp900547615
CAZyme ID MGYG000001224_00390
CAZy Family CBM48
CAZyme Description 1,4-alpha-glucan branching enzyme GlgB
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
623 72296.99 6.5837
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000001224 2725893 MAG Austria Europe
Gene Location Start: 42871;  End: 44742  Strand: -

Full Sequence      Download help

MEFTQLDQYL  FGQGTHYEIY  KKLGAHPTSV  KRKKGVYFAV  WAPNAKSVSV  VGEFNNWDTE60
ANPMQKAGDI  GVYELFVPGA  KEGQLYKFYI  KGAHGEELYK  ADPYANEAEL  RPGTASRITD120
ISDYKWTDDK  WMKQRENFDE  KVQPVSIYEV  HIGSWMKHSP  TEENEKGFYN  YREVAPRLAE180
YVKEMGYTHV  ELMGIAEHPF  DGSWGYQVTG  YYAPTSRYGT  PQDFKYLVDY  LHRHKIGVIL240
DWVPAHFPKD  AHGLVNFDGT  AVYEHEDPRQ  GEHPDWGTKI  YNYGRPEVKN  FLIANALFWV300
EECHVDGLRV  DAVASMLYLD  YGKKDGEWVA  NKYGENKNLE  AIEFFKHLNT  VVLGRNHGTM360
MIAEESTAWP  KVTGKAEEDG  LGFSLKWNMG  WMNDFLEYMK  LDPYFRKFNH  NKMTFSMVYA420
YSEKYILVLS  HDEVVHLKCS  MLSKMPGELD  EKFKNLKAGY  AFMFCHPGKK  LLFMGQDFGQ480
LREWSEEREL  DWFLLGEDGH  RNLKSFVKDL  LHIYKKYPAL  YAGDNNPEGF  EWINANDGDR540
SIFSFVRNSP  TKRNNVLVVC  NFTPVARPDY  RVGVPKKKNY  KLILDENGMT  DPKIFKAEKK600
ECDNRQFSFA  YPLPAYGIAV  FLY623

Enzyme Prediction      help

EC 2.4.1.18

CAZyme Signature Domains help

Created with Snap316293124155186218249280311342373404436467498529560591180480GH1322108CBM48
Family Start End Evalue family coverage
GH13 180 480 1e-153 0.9966777408637874
CBM48 22 108 2.9e-22 0.9078947368421053

CDD Domains      download full data without filtering help

Created with Snap3162931241551862182492803113423734044364674985295605915621PRK147054621GlgB4621PRK054023621PRK123137620branching_enzym
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
PRK14705 PRK14705 0.0 5 621 605 1220
glycogen branching enzyme; Provisional
COG0296 GlgB 0.0 4 621 7 626
1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK05402 PRK05402 0.0 4 621 101 721
1,4-alpha-glucan branching protein GlgB.
PRK12313 PRK12313 0.0 3 621 7 625
1,4-alpha-glucan branching protein GlgB.
TIGR01515 branching_enzym 0.0 7 620 1 616
alpha-1,4-glucan:alpha-1,4-glucan 6-glycosyltransferase. This model describes the glycogen branching enzymes which are responsible for the transfer of chains of approx. 7 alpha(1--4)-linked glucosyl residues to other similar chains (in new alpha(1--6) linkages) in the biosynthesis of glycogen. This enzyme is a member of the broader amylase family of starch hydrolases which fold as (beta/alpha)8 barrels, the so-called TIM-barrel structure. All of the sequences comprising the seed of this model have been experimentally characterized. This model encompasses both bacterial and eukaryotic species. No archaea have this enzyme, although Aquifex aolicus does. Two species, Bacillus thuringiensis and Clostridium perfringens have two sequences each which are annotated as amylases. These annotations are aparrently in error. GP|18143720 from C. perfringens, for instance, contains the note "674 aa, similar to gp:A14658_1 amylase (1,4-alpha-glucan branching enzyme (EC 2.4.1.18) ) from Bacillus thuringiensis (648 aa); 51.1% identity in 632 aa overlap." A branching enzyme from Porphyromonas gingivales, OMNI|PG1793, appears to be more closely related to the eukaryotic species (across a deep phylogenetic split) and may represent an instance of lateral transfer from this species' host. A sequence from Arabidopsis thaliana, GP|9294564, scores just above trusted, but appears either to contain corrupt sequence or, more likely, to be a pseudogene as some of the conserved catalytic residues common to the alpha amylase family are not conserved here. [Energy metabolism, Biosynthesis and degradation of polysaccharides]

CAZyme Hits      help

Created with Snap3162931241551862182492803113423734044364674985295605911623QCU01659.1|CBM48|GH13_91623CBL22762.1|CBM48|GH13_91623QIB55783.1|CBM48|GH13_91623QMW76350.1|CBM48|GH13_91623ASU30768.1|CBM48|GH13_9
Hit ID E-Value Query Start Query End Hit Start Hit End
QCU01659.1 0.0 1 623 5 628
CBL22762.1 0.0 1 623 5 628
QIB55783.1 0.0 1 623 7 636
QMW76350.1 0.0 1 623 7 636
ASU30768.1 0.0 1 623 7 636

PDB Hits      download full data without filtering help

Created with Snap31629312415518621824928031134237340443646749852956059145805GQW_A45805GR5_A45805GQZ_A45805GQU_A45805GR2_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
5GQW_A 3.59e-233 4 580 130 723
Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A 5.09e-233 4 580 130 723
Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A 7.20e-233 4 580 130 723
Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A 1.02e-232 4 580 130 723
Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A 1.44e-232 4 580 130 723
Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits      download full data without filtering help

Created with Snap3162931241551862182492803113423734044364674985295605917623sp|P30539|GLGB_BUTFI5589sp|Q2JT08|GLGB_SYNJA4587sp|Q8DLB8|GLGB_THEVB4587sp|Q608L5|GLGB_METCA5589sp|Q2JK68|GLGB_SYNJB
Hit ID E-Value Query Start Query End Hit Start Hit End Description
P30539 4.50e-297 7 623 11 632
1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q2JT08 3.10e-241 5 589 119 712
1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=glgB PE=3 SV=1
Q8DLB8 5.45e-241 4 587 107 707
1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q608L5 2.10e-239 4 587 106 683
1,4-alpha-glucan branching enzyme GlgB OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=glgB PE=3 SV=1
Q2JK68 7.41e-238 5 589 119 712
1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000045 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000001224_00390.