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CAZyme Information: MGYG000004471_00179

You are here: Home > Sequence: MGYG000004471_00179

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species UBA1394 sp900554975
Lineage Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; UBA1394; UBA1394 sp900554975
CAZyme ID MGYG000004471_00179
CAZy Family CBM48
CAZyme Description 1,4-alpha-glucan branching enzyme GlgB
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
690 80108.74 6.1875
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000004471 1997651 MAG Israel Asia
Gene Location Start: 47254;  End: 49326  Strand: -

Full Sequence      Download help

MDNNYSGNIP  ETYKFPLYLF  YQGKNCEAYK  FFGVHKVDID  GKKYHCFRVW  APNAVKVSVT60
GDFNNWNKHS  DPMKKISDGI  WECFIPELEQ  FSAYKYCIET  QDGRFLMKAD  PYAAHYETRP120
GTASKIYESS  FVWNDKKWMD  EKSSRIIYKG  PVNIYEVHLG  SWKLNEDGSH  LGYVTFAENI180
IPYMKKMSYT  HIELMPLTEF  PFDGSWGYQV  TGYYAPTSRF  GTPDDFRKMI  EMFHEAEIGV240
ILDWVPAHFP  KDASGLYEFD  GTCCYEYADE  RKREHKSWGT  RVFDYGKGEV  CSFLISSANY300
WLSEYHIDGI  RVDAVASMLY  LDYDRRDGEW  CPNVNGGKEN  LEAIEFFKHL  NEAVFEKHPD360
VLMIAEESTA  WPMVTKPTDI  GGLGFNFKWN  MGWMNDMLQY  MSLDPIYRAF  NHDKLTFSFF420
YCFSENYILP  ISHDEVVHGK  CSMLEKMPGT  YEEKCSSYKA  FLAYMMAHPG  KKLLFMGQEF480
GQSCEWNYKT  QLDWAALEEI  NHKNIQEFSE  KLNRFYLENA  PLWQNDDSWQ  GFSWISNDDY540
KQSVIAFRRF  DDNGDEIIVV  CNFVPVARHD  YKIGVPYEGE  YKLVFNSDAK  EFGGSGITAE600
SMKSVSYIMH  GFDDSISIDI  APLSVIYLKY  VPKKKTVEKT  PEKKTEKGPE  KASVKKHTKH660
KGKSSGNVTH  LIPGLNYNNT  DIKNLKSVKK  690

Enzyme Prediction      help

EC 2.4.1.18

CAZyme Signature Domains help

Created with Snap3469103138172207241276310345379414448483517552586621655182482GH1332115CBM48
Family Start End Evalue family coverage
GH13 182 482 5.4e-154 0.9966777408637874
CBM48 32 115 5.5e-16 0.881578947368421

CDD Domains      download full data without filtering help

Created with Snap346910313817220724127631034537941444848351755258662165514636PRK1231331650PRK147069629PRK14705119518AmyAc_Glg_BE15633PRK05402
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
PRK12313 PRK12313 0.0 14 636 9 633
1,4-alpha-glucan branching protein GlgB.
PRK14706 PRK14706 0.0 31 650 26 638
glycogen branching enzyme; Provisional
PRK14705 PRK14705 0.0 9 629 600 1221
glycogen branching enzyme; Provisional
cd11322 AmyAc_Glg_BE 0.0 119 518 2 402
Alpha amylase catalytic domain found in the Glycogen branching enzyme (also called 1,4-alpha-glucan branching enzyme). The glycogen branching enzyme catalyzes the third step of glycogen biosynthesis by the cleavage of an alpha-(1,4)-glucosidic linkage and the formation a new alpha-(1,6)-branch by subsequent transfer of cleaved oligosaccharide. They are part of a group called branching enzymes which catalyze the formation of alpha-1,6 branch points in either glycogen or starch. This group includes proteins from bacteria, eukaryotes, and archaea. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
PRK05402 PRK05402 0.0 15 633 103 726
1,4-alpha-glucan branching protein GlgB.

CAZyme Hits      help

Created with Snap34691031381722072412763103453794144484835175525866216559635CBL16844.1|CBM48|GH13_916634QEY35933.1|CBM48|GH13_98634CBL34144.1|CBM48|GH13_98634CBK95803.1|CBM48|GH13_916635CAB1241654.1|CBM48|GH13_9
Hit ID E-Value Query Start Query End Hit Start Hit End
CBL16844.1 0.0 9 635 2 631
QEY35933.1 1.92e-310 16 634 11 632
CBL34144.1 8.33e-309 8 634 4 633
CBK95803.1 1.18e-308 8 634 4 633
CAB1241654.1 5.75e-301 16 635 11 637

PDB Hits      download full data without filtering help

Created with Snap3469103138172207241276310345379414448483517552586621655176345GR1_A176345GQW_A176345GR5_A176345GR0_A176345GQZ_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
5GR1_A 6.42e-215 17 634 134 777
Crystalstructure of branching enzyme Y500A/D501A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142],5GR6_A Crystal structure of branching enzyme Y500A/D501A double mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQW_A 6.42e-215 17 634 134 777
Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A 9.08e-215 17 634 134 777
Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR0_A 9.08e-215 17 634 134 777
Crystalstructure of branching enzyme D501A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A 1.29e-214 17 634 134 777
Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits      download full data without filtering help

Created with Snap346910313817220724127631034537941444848351755258662165515630sp|O66936|GLGB_AQUAE9629sp|B8CVY1|GLGB_HALOH9632sp|Q1AZ86|GLGB_RUBXD10636sp|Q608L5|GLGB_METCA18634sp|Q46LW6|GLGB_PROMT
Hit ID E-Value Query Start Query End Hit Start Hit End Description
O66936 7.62e-235 15 630 10 628
1,4-alpha-glucan branching enzyme GlgB OS=Aquifex aeolicus (strain VF5) OX=224324 GN=glgB PE=3 SV=1
B8CVY1 2.17e-234 9 629 5 626
1,4-alpha-glucan branching enzyme GlgB OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) OX=373903 GN=glgB PE=3 SV=1
Q1AZ86 1.09e-229 9 632 89 721
1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
Q608L5 1.69e-225 10 636 103 731
1,4-alpha-glucan branching enzyme GlgB OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=glgB PE=3 SV=1
Q46LW6 3.17e-224 18 634 112 754
1,4-alpha-glucan branching enzyme GlgB OS=Prochlorococcus marinus (strain NATL2A) OX=59920 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000062 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000004471_00179.