CAZyme Information: MGYG000003140_00276

Basic Information

• Species: Pauljensenia sp000758755
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Pauljensenia; Pauljensenia sp000758755
• CAZyme ID: MGYG000003140_00276
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1753		186890.19	4.7139

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003140	1808392	MAG	United States	North America

• Gene Location: Start: 21519; End: 26780 Strand: -

Full Sequence      

MIDKRVFWRPLAILGALALSLPGSAVAHATEGATDTPVILNAPTTISAGQLRATVSHEFP
QVTGYTFAGNPVGGRAETLHQVTIDGNQYTVDTVEAQRVSDQKVAYTVTFVGSEITMKAE
IEVQEITSHAQGTEGAKRPTLTFRITEMTGAHTVEIPGHGLVSVNADQGGAYACGITGVS
RGAANGDSYAGVDDTFASITASTPIDYYEQPSAYLMVNTNKVAVGMETNATYDEPHGYET
NWNSRWKRQVIEQNGIKTLIAQNGQWTYRSEAATDAIGDEERPYTTLVFTGDANSSGSVD
WQDAAVAYADITPEITGAADNHKWVVTHIPFDFGSAATHPFLQIADDVKRVSLATDGLGQ
RVMVKGYASEGHDSGHMDYGGNINTRAGGEADFGTLFTSTKDVNAIYGVHVNTTEAYPEA
NSFRSLPFTGGRGWNWLNQSYYVNQRDDLGNGGAVNRFQELRNQFPLSKYPNFRWIYIDV
YYGSGWQADRLGNELNKMGWEVGSEWADRFERHSLWSHWSNDEHYGGATNKGLNSQVIRF
VDNANKDNWNPNVVLGYPQIVEFEGWTGHQDQDAFYRNIWSNNLPSKFLQNSRIMRYDTA
DAGDGKTKHTYTFANGTVASGSTAVTAQTEASYVVGTIQADMSSSREFVYDGATVLRGDS
YLLPWKDNGTSAGADRLYYYNPAGTATEWTLTKSYQGVGSLALYRLTDQGRVKVTDLAVS
GGKITIPASVYDGVAEGDRDHTAFVLVPENAPAAVSTPAWGAGTVFADPGFNSGSLSGYT
TTGSVSVVKDPHGDPVAQLGAGKASLSQEMTLNAGTYQGSAWVQINDGKTRKVTISASGD
GVTSAGSQQRNGDLGATRDKPVTTVFEDFENIAEGWGPFVKGDAGNITDPRTVLASLNAP
YTQRGGVLPDRSRKATDDAIAGHWSLKSHEENGGLVYRTVPQTVNLQAGHRYRISYDYDN
AVSGSYYWVTAYDSAGADGVSANYLDSTLLPQTERPSHFVEELNVGACGSYWVGLYNVQG
GKSGNDFSIDNFRVEDLGTTADVPACAAASIATQGSAIEGKTVQVTTTLVNNESADVTNV
RSELSLPAGWSAQATSPTTAETLAKGETFTTTWTVSIPEGAGGNAVTLTHQAHYRIGDED
RTAVGTTSVTALGSIRSNAVNYLSDIPFSSTWNSNGWGPVERDSEVGEDQAGDGDPISMN
DQPYAKGLGAHAPSDLGFELDGKCTTFKATVGVQDGQWSGSVTFHVIGSTDGSSWSDVVP
ETSVIRGGSEPRDISGNVRGMKYIRLIVGDGGNGNGNDHANWGNARVLCGNATEGGDTPA
PEPTPTLTLKDYTGALQLVSAPAAYEANPASNMFDKDLTTFYDKDWVNLTDHPSTIDLAL
FEGSDPTGAQPVSIGGLSIAGRAGQANGRPKAYEVYVGQDAANINQKVAEGTLRNTALAQ
RITFDEAVNAKYVRIRVLSNYKTKADQPDGLLAIAELGVLVPDGTTRAAVSGSDRSAGTR
SARRARSLDDEQALTVNTNAEGLVGQNYLDVQTVNATDPAANPETVIDRTWAENWMAADQ
KHRTNSDPSSFQRVPVTFTVAKDGTKVRFTIAADEGDAPVLFDNLRMVKIERPATNDLLG
VDCMADPTPEACTTMRANARIGLSTALSVSLPAPTPDPTADPSAQPTTDPTTQPSGDPSP
QPSADPSQSGSQQASPATQEQAGAASAKDGKKKSTRKSKKSSLARTGGETSAILISAALL
TAAGYALRRRRMN

Enzyme Prediction     

No EC number prediction in MGYG000003140_00276.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	50	728	2.4e-165	0.9801980198019802
CBM51	1162	1306	1.8e-34	0.9776119402985075

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	3.27e-127	302	576	1	273	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	5.43e-117	317	599	3	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam18080	Gal_mutarotas_3	2.66e-50	46	301	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam08305	NPCBM	8.54e-39	1160	1307	2	135	NPCBM/NEW2 domain. This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins. This domain has also been called the NEW2 domain (Naumoff DG. Phylogenetic analysis of alpha-galactosidases of the GH27 family. Molecular Biology (Engl Transl). (2004)38:388-399.)
smart00776	NPCBM	4.53e-30	1161	1307	5	144	This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VEG53803.1	0.0	9	1645	8	1630
QQM68206.1	0.0	7	1645	6	1635
QQO77976.1	0.0	9	1645	14	1640
AVM61293.1	0.0	9	1645	14	1640
QDW61529.1	9.15e-302	44	1346	73	1371

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QEP_A	2.21e-105	45	1037	11	1116	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6SH9_B	2.29e-105	45	1037	11	1116	EngBFDARPin Fusion 4b D12 [Bifidobacterium longum subsp. longum JCM 1217]
6QFK_A	2.29e-105	45	1037	11	1116	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]
6QEV_B	2.29e-105	45	1037	11	1116	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
2ZXQ_A	2.87e-105	45	1037	26	1131	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A9WNA0	5.32e-240	9	1341	13	1330	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2
Q8DR60	3.67e-97	45	1040	333	1421	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
Q2MGH6	4.86e-97	45	1040	333	1421	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000422	0.998661	0.000226	0.000291	0.000195	0.000169

TMHMM  Annotations     

start	end
1730	1747