CAZyme Information: MGYG000003993_00022

Basic Information

• Species: Alistipes sp900550925
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; Alistipes sp900550925
• CAZyme ID: MGYG000003993_00022
• CAZy Family: GH9
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
821		92471.52	4.7212

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003993	1895769	MAG	United Kingdom	Europe

• Gene Location: Start: 24258; End: 26723 Strand: -

Full Sequence      

MAACLLIAGSASATSWIRTNLIGYTNALPKTAVLLSDQPLTVTTFEVCDARSGRTVYTGT
AVEKDASVWAMKSAYRLPFSDFSQEGAYYIKVGDTRSENFRIGDDIYTGAGEVPLYYMRQ
QRCGDNPYTGELCHQHDGYIVEHPTRNGEYIDVRGGWHDATDQLQYLTTSANATFQMMFA
YYKNPDQFEDKFDASGRKGANGVPDILDEARWGLEWMKRMNPDSAFMFNQIADDRDHAGI
REPHTDKVDYGWGPGTGRPVYFISGEVQGLSKYKNRTTGVSSSAAKYASAFALGSVIFEK
FDPEFAESMKKKAVDAMAYAEAIPGACQTACVVSPYFYEEDNWIDDMELAAVALHLMEPE
NEYWLQRANYWGDLEPITPWMETGKARHYQWYPFVNLGHYLIAAEGTEAQKAKFAGYMRE
GLQHILDRAGDDPFVHGVPYLWCSNNFTFGALTQLRLYNELTGDDTFLEMEAALLDWIFG
CNPWGTSMICDYPKGVDTPTDPHAIYTVTRGENVYGGLVDGPVYKYINDGLQGVSAGDSD
PFLPFQNGIAVYHDYFGDYSTNEPTMDGTASMTYYLSTLDKIDDQVKKNDKYAGAVYDEF
GAIRRMNPDQKRVYLVFTGDVNFEGVPTVLKTLKKHGIKASWFLTGNCVRTNPKQVKAIM
KAGHYIGPHSDKHLLYAEWDADRKSLVTPQEIREDLDANMAEFKAFGIDVEKIRAMIPPY
EHYNKASNGVLVQYGFIPVNLTEGTYTNADYTTPDMKNYRSSDAILKDFWAKEKREGLNG
AIILIHPGTESSRKDKLYDRLDQIITQLEEKGYDFGTFDEE

Enzyme Prediction     

No EC number prediction in MGYG000003993_00022.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH9	112	576	5.9e-71	0.9808612440191388
CE4	607	736	2.7e-16	0.9

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00759	Glyco_hydro_9	6.86e-47	115	576	7	374	Glycosyl hydrolase family 9.
COG0726	CDA1	2.04e-25	553	821	8	256	Peptidoglycan/xylan/chitin deacetylase, PgdA/CDA1 family [Carbohydrate transport and metabolism, Cell wall/membrane/envelope biogenesis].
cd10917	CE4_NodB_like_6s_7s	6.58e-24	611	808	1	170	Catalytic NodB homology domain of rhizobial NodB-like proteins. This family belongs to the large and functionally diverse carbohydrate esterase 4 (CE4) superfamily, whose members show strong sequence similarity with some variability due to their distinct carbohydrate substrates. It includes many rhizobial NodB chitooligosaccharide N-deacetylase (EC 3.5.1.-)-like proteins, mainly from bacteria and eukaryotes, such as chitin deacetylases (EC 3.5.1.41), bacterial peptidoglycan N-acetylglucosamine deacetylases (EC 3.5.1.-), and acetylxylan esterases (EC 3.1.1.72), which catalyze the N- or O-deacetylation of substrates such as acetylated chitin, peptidoglycan, and acetylated xylan. All members of this family contain a catalytic NodB homology domain with the same overall topology and a deformed (beta/alpha)8 barrel fold with 6- or 7 strands. Their catalytic activity is dependent on the presence of a divalent cation, preferably cobalt or zinc, and they employ a conserved His-His-Asp zinc-binding triad closely associated with the conserved catalytic base (aspartic acid) and acid (histidine) to carry out acid/base catalysis. Several family members show diversity both in metal ion specificities and in the residues that coordinate the metal.
cd10950	CE4_BsYlxY_like	9.82e-24	607	820	2	186	Putative catalytic NodB homology domain of uncharacterized protein YlxY from Bacillus subtilis and its bacterial homologs. The Bacillus subtilis genome contains six polysaccharide deacetylase gene homologs: pdaA, pdaB (previously known as ybaN), yheN, yjeA, yxkH and ylxY. This family is represented by Bacillus subtilis putative polysaccharide deacetylase BsYlxY, encoded by the ylxY gene, which is a member of the carbohydrate esterase 4 (CE4) superfamily. Although its biological function still remains unknown, BsYlxY shows high sequence homology to the catalytic domain of Bacillus subtilis pdaB gene encoding a putative polysaccharide deacetylase (BsPdaB), which is essential for the maintenance of spores after the late stage of sporulation and is highly conserved in spore-forming bacteria. However, disruption of the ylxY gene in B. subtilis did not cause any sporulation defect. Moreover, the Asp residue in the classical His-His-Asp zinc-binding motif of CE4 esterases is mutated to a Val residue in this family. Other catalytically relevant residues of CE4 esterases are also not conserved, which suggest that members of this family may be inactive.
cd02850	E_set_Cellulase_N	3.17e-22	16	102	1	86	N-terminal Early set domain associated with the catalytic domain of cellulase. E or "early" set domains are associated with the catalytic domain of cellulases at the N-terminal end. Cellulases are O-glycosyl hydrolases (GHs) that hydrolyze beta 1-4 glucosidic bonds in cellulose. They are usually categorized into either exoglucanases, which sequentially release terminal sugar units from the cellulose chain, or endoglucanases, which also attack the chain internally. The N-terminal domain of cellulase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIK60909.1	0.0	4	818	14	826
QIK55492.1	0.0	13	818	22	826
AGY53377.1	0.0	12	815	21	828
AHW60805.1	3.31e-306	17	815	24	812
QIA07887.1	3.67e-303	17	815	25	813

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3X17_A	2.99e-41	17	574	18	551	Crystalstructure of metagenome-derived glycoside hydrolase family 9 endoglucanase [uncultured bacterium],3X17_B Crystal structure of metagenome-derived glycoside hydrolase family 9 endoglucanase [uncultured bacterium]
5U2O_A	2.28e-35	29	577	6	537	Crystalstructure of Zn-binding triple mutant of GH family 9 endoglucanase J30 [Thermobacillus composti KWC4]
5U0H_A	6.00e-32	29	577	6	537	Crystalstructure of GH family 9 endoglucanase J30 [Thermobacillus composti KWC4]
1UT9_A	7.36e-21	17	584	7	609	ChainA, CELLULOSE 1,4-BETA-CELLOBIOSIDASE [Acetivibrio thermocellus]
1RQ5_A	1.60e-19	17	581	7	606	ChainA, Cellobiohydrolase [Acetivibrio thermocellus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P14090	2.05e-23	17	577	341	905	Endoglucanase C OS=Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) OX=590998 GN=cenC PE=1 SV=2
P0C2S1	5.11e-20	17	583	214	815	Cellulose 1,4-beta-cellobiosidase OS=Acetivibrio thermocellus OX=1515 GN=celK PE=1 SV=1
P23658	1.71e-19	17	579	4	545	Cellodextrinase OS=Butyrivibrio fibrisolvens OX=831 GN=ced1 PE=1 SV=1
A3DCH1	2.03e-19	17	583	214	815	Cellulose 1,4-beta-cellobiosidase OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celK PE=3 SV=1
Q04729	6.18e-14	598	820	54	252	Uncharacterized 30.6 kDa protein in fumA 3'region OS=Geobacillus stearothermophilus OX=1422 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.502377	0.490972	0.005653	0.000526	0.000214	0.000255

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003993_00022.