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CAZyme Information: MGYG000000429_00127

You are here: Home > Sequence: MGYG000000429_00127

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species UBA7597 sp900542935
Lineage Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; Borkfalkiaceae; UBA7597; UBA7597 sp900542935
CAZyme ID MGYG000000429_00127
CAZy Family GH112
CAZyme Description D-galactosyl-beta-1->4-L-rhamnose phosphorylase
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
738 MGYG000000429_1|CGC2 82716.41 6.5092
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000429 1972857 MAG Sweden Europe
Gene Location Start: 169983;  End: 172199  Strand: +

Full Sequence      Download help

MEQKSEAARR  RKPKGDFTLP  GEAGYEKLTL  RLAEKWGADV  VRDSDGTQLS  PEILSAGYGV60
YSTICVIREH  NDFAKAHPET  QAEAPLYSLP  VAAESETLEI  NLLNGYFSQQ  FEIDERECSQ120
KYWQAFDRTE  NKELPREEWT  YLGGGKALIK  HAKKYHEYSV  NFFAFRIWEE  ISMYNHITNG180
WQKEHLRQLD  PVHPIVRGYL  KDYLRRWCAA  HPATNVVRFT  SLFYNFVWIW  GGDENNRFLF240
TDWASYDFTA  SPAMLENYEK  STGGKISAED  FINGGKRRST  HTLSDETKRK  YMRFVQNFVA300
DFAAELVQIV  HDAGKKAYMF  YDDSWVGSEP  YSESFARIGF  DGIIKCVFSA  YEARMCADVP360
GVETHELRLH  PYLFPTGLTG  EPVFAAGGDP  VTPAKQYWTA  VRRALLRRCA  DRIGLGGYLH420
LTQNFPDFCD  YIETLADEFR  EIKALHKGGA  PYALPVKISV  LTHWGKLRSW  TCGGHYHEHP480
DLDLINLLES  IAGLPVQTEF  IDFAQLKNGD  FTGNVLLVAG  LKNSAYGGGS  EWNDVKTYES540
AAKFVHGGGA  LICVNEASET  DGFSTNLRLA  PLLGVDLDRG  DFNCRGKYPA  TAEREEISVG600
TFTGKKEAFI  FAPDVRILKN  AEARAGEEKT  FAVAVRNVGK  GFATYVSSYK  HTPENAAALL660
SLLLRLRNLS  AAENYLSDNP  YVDCAYFEKE  KKLALANVSG  VSQAARISLP  GGGKTFTLPP720
AGIKIVPVGK  IRAQEKEE738

Enzyme Prediction      help

EC 2.4.1.247

CAZyme Signature Domains help

Created with Snap367311014718422125829533236940544247951655359062766470115725GH112
Family Start End Evalue family coverage
GH112 15 725 7.3e-229 0.9958041958041958

CDD Domains      download full data without filtering help

Created with Snap367311014718422125829533236940544247951655359062766470115454Lact_bio_phlase14724TIGR02336457654LBP_M675725LBP_C
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
pfam09508 Lact_bio_phlase 0.0 15 454 1 434
Lacto-N-biose phosphorylase N-terminal TIM barrel domain. The gene which codes for this protein in gut-bacteria is located in a novel putative operon for galactose metabolism. The protein appears to be a carbohydrate-processing phosphorolytic enzyme (EC:2.4.1.211), unlike either glycoside hydrolases or glycoside lyase. Intestinal colonisation by bifidobacteria is important for human health, especially in pediatrics, because colonisation seems to prevent infection by some pathogenic bacteria that cause diarrhoea or other illnesses. The operon seems to be involved in intestinal colonisation by bifidobacteria mediated by metabolism of mucin sugars. In addition, it may also resolve the question of the nature of the bifidus factor in human milk as the lacto-N-biose structure found in milk oligosaccharides.
TIGR02336 TIGR02336 2.08e-177 14 724 3 716
1,3-beta-galactosyl-N-acetylhexosamine phosphorylase. Members of this family are found in phylogenetically diverse bacteria, including Clostridium perfringens (in the Firmicutes), Bifidobacterium longum and Propionibacterium acnes (in the Actinobacteria), and Vibrio vulnificus (in the Proteobacteria), most of which occur as mammalian pathogens or commensals. The nominal activity, 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase (EC 2.4.1.211), varies somewhat from instance to instance in relative rates for closely related substrates. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
pfam17385 LBP_M 1.43e-38 457 654 1 204
Lacto-N-biose phosphorylase central domain. The gene which codes for this protein in gut-bacteria is located in a novel putative operon for galactose metabolism. The protein appears to be a carbohydrate-processing phosphorolytic enzyme (EC:2.4.1.211), unlike either glycoside hydrolases or glycoside lyase. Intestinal colonisation by bifidobacteria is important for human health, especially in pediatrics, because colonisation seems to prevent infection by some pathogenic bacteria that cause diarrhoea or other illnesses. The operon seems to be involved in intestinal colonisation by bifidobacteria mediated by metabolism of mucin sugars. In addition, it may also resolve the question of the nature of the bifidus factor in human milk as the lacto-N-biose structure found in milk oligosaccharides.
pfam17386 LBP_C 4.85e-09 675 725 1 52
Lacto-N-biose phosphorylase C-terminal domain. The gene which codes for this protein in gut-bacteria is located in a novel putative operon for galactose metabolism. The protein appears to be a carbohydrate-processing phosphorolytic enzyme (EC:2.4.1.211), unlike either glycoside hydrolases or glycoside lyase. Intestinal colonisation by bifidobacteria is important for human health, especially in pediatrics, because colonisation seems to prevent infection by some pathogenic bacteria that cause diarrhoea or other illnesses. The operon seems to be involved in intestinal colonisation by bifidobacteria mediated by metabolism of mucin sugars. In addition, it may also resolve the question of the nature of the bifidus factor in human milk as the lacto-N-biose structure found in milk oligosaccharides.

CAZyme Hits      help

Created with Snap367311014718422125829533236940544247951655359062766470115712ADY12199.1|GH11214716AOZ97581.1|GH11212730BCJ96439.1|GH1121728ABX42289.1|GH112|2.4.1.24714733QLG36879.1|GH112
Hit ID E-Value Query Start Query End Hit Start Hit End
ADY12199.1 1.04e-249 15 712 8 696
AOZ97581.1 1.13e-243 14 716 9 709
BCJ96439.1 1.25e-242 12 730 5 721
ABX42289.1 5.19e-242 1 728 1 721
QLG36879.1 6.71e-238 14 733 6 723

PDB Hits      download full data without filtering help

Created with Snap3673110147184221258295332369405442479516553590627664701157233WFZ_A157232ZUS_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
3WFZ_A 5.64e-116 15 723 5 746
Crystalstructure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase C236Y Mutant [Bifidobacterium longum subsp. longum JCM 1217],3WFZ_B Crystal structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase C236Y Mutant [Bifidobacterium longum subsp. longum JCM 1217],3WFZ_C Crystal structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase C236Y Mutant [Bifidobacterium longum subsp. longum JCM 1217],3WFZ_D Crystal structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase C236Y Mutant [Bifidobacterium longum subsp. longum JCM 1217]
2ZUS_A 1.18e-114 15 723 5 746
Crystalstructure of Galacto-N-biose/Lacto-N-biose I phosphorylase [Bifidobacterium longum],2ZUS_B Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase [Bifidobacterium longum],2ZUS_C Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase [Bifidobacterium longum],2ZUS_D Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase [Bifidobacterium longum],2ZUT_A Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GalNAc [Bifidobacterium longum],2ZUT_B Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GalNAc [Bifidobacterium longum],2ZUT_C Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GalNAc [Bifidobacterium longum],2ZUT_D Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GalNAc [Bifidobacterium longum],2ZUU_A Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc [Bifidobacterium longum],2ZUU_B Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc [Bifidobacterium longum],2ZUU_C Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc [Bifidobacterium longum],2ZUU_D Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc [Bifidobacterium longum],2ZUV_A Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc, Ethylene glycol, and nitrate [Bifidobacterium longum],2ZUV_B Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc, Ethylene glycol, and nitrate [Bifidobacterium longum],2ZUW_A Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc and sulfate [Bifidobacterium longum],2ZUW_B Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc and sulfate [Bifidobacterium longum],2ZUW_C Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc and sulfate [Bifidobacterium longum],2ZUW_D Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc and sulfate [Bifidobacterium longum]

Swiss-Prot Hits      download full data without filtering help

Created with Snap36731101471842212582953323694054424795165535906276647011728sp|A9KHK4|GLRP_LACP715723sp|E8MF13|LNPA_BIFL215697sp|A9KIW5|GAHP2_LACP715718sp|A9KQ75|GAHP1_LACP7
Hit ID E-Value Query Start Query End Hit Start Hit End Description
A9KHK4 1.04e-242 1 728 1 721
D-galactosyl-beta-1->4-L-rhamnose phosphorylase OS=Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) OX=357809 GN=Cphy_1920 PE=1 SV=1
E8MF13 5.29e-114 15 723 5 746
1,3-beta-galactosyl-N-acetylhexosamine phosphorylase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=lnpA PE=1 SV=1
A9KIW5 1.22e-110 15 697 9 691
1,3-beta-galactosyl-N-acetylhexosamine phosphorylase Cphy0577 OS=Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) OX=357809 GN=Cphy_0577 PE=1 SV=1
A9KQ75 2.49e-104 15 718 7 709
1,3-beta-galactosyl-N-acetylhexosamine phosphorylase Cphy3030 OS=Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) OX=357809 GN=Cphy_3030 PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000049 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000000429_00127.