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CAZyme Information: MGYG000000121_00810

You are here: Home > Sequence: MGYG000000121_00810

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Eubacterium callanderi
Lineage Bacteria; Firmicutes_A; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; Eubacterium callanderi
CAZyme ID MGYG000000121_00810
CAZy Family GH13
CAZyme Description 1,4-alpha-glucan branching enzyme GlgB
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
664 MGYG000000121_2|CGC2 76403.41 6.0408
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000121 4357692 Isolate Canada North America
Gene Location Start: 215681;  End: 217675  Strand: -

Full Sequence      Download help

MSSDNLEILN  IDPYLERYKS  AIEWRNNRFN  MKKKALLKNK  KSLSDFADGY  LYFGFHQKAE60
GWVYREWAPN  AKSVFLIGDF  NGWDRDATPL  KPLGSGRWEV  FLPGKKALPH  GSRVKVHIKT120
KDQSFDRVPL  YCKRVIQDKN  TFAFDGQIWN  PEQPYKWHDK  AFHPDQSVPP  LIYEAHIGIA180
GESPEVSTFK  EFTQNTLPHI  AGLGYNAVQL  MAIMEHPYYA  SFGYQVSNFF  AVSSRFGTPE240
DLKALIDTAH  HLGIAVILDL  VHSHASRNVL  DGIGEFDGTD  YQFFHAGPEG  DHPAWGSKVF300
NYDKPEVLHF  LLSNIKFWLD  EYHFDGFRFD  GVTSILYHNH  GLGVNFNSYD  QYFSSNTDMS360
GLTYLQMASA  LAKEIKPDCF  LIAEDMSGLP  GMALPVSQGG  LGFDYRLGMG  LPDFWIHTLR420
DLRDEDWDLN  ALWHELTQRR  PGEKVIGYAE  SHDQAIVGDK  TIMFWLADQD  MYSDMNVFNQ480
NPVIERALAL  HKMIRLITCT  CAGEGYMNFM  GNEFGHPEWI  DFPREENGWS  YQYARRQWHL540
AEDTNLRYRY  LQDFDRAMIH  FVKETKLLEY  PSELLLIDTW  AKLLLYSKGP  YLFAFNFHPT600
KDFNGFIPLP  DSNGYQPLLN  TESRDFGGWM  DTEPDLIDAK  ANCPGRLTTL  YIPKRSAAVY660
APAR664

Enzyme Prediction      help

EC 2.4.1.18

CAZyme Signature Domains help

Created with Snap336699132166199232265298332365398431464498531564597630221512GH13
Family Start End Evalue family coverage
GH13 221 512 6.4e-144 0.9965870307167235

CDD Domains      download full data without filtering help

Created with Snap3366991321661992322652983323653984314644985315645976304663PLN02447151556AmyAc_bac_euk_BE98664PLN0296094664PLN0324450662GlgB
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
PLN02447 PLN02447 0.0 4 663 58 731
1,4-alpha-glucan-branching enzyme
cd11321 AmyAc_bac_euk_BE 0.0 151 556 1 406
Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
PLN02960 PLN02960 9.21e-159 98 664 316 893
alpha-amylase
PLN03244 PLN03244 1.77e-133 94 664 327 868
alpha-amylase; Provisional
COG0296 GlgB 7.81e-111 50 662 23 628
1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].

CAZyme Hits      help

Created with Snap3366991321661992322652983323653984314644985315645976301664ADO37953.1|CBM48|GH13_81664ARD65756.1|CBM48|GH13_81661QCT73488.1|CBM48|GH13_81661ALU15473.1|CBM48|GH13_82578QQY55903.1|CBM48|GH13_8
Hit ID E-Value Query Start Query End Hit Start Hit End
ADO37953.1 0.0 1 664 1 664
ARD65756.1 0.0 1 664 1 664
QCT73488.1 0.0 1 661 1 661
ALU15473.1 0.0 1 661 1 661
QQY55903.1 2.21e-280 2 578 3 579

PDB Hits      download full data without filtering help

Created with Snap33669913216619923226529833236539843146449853156459763085584BZY_A155585CLT_A45753AMK_A45753VU2_A45757ML5_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
4BZY_A 6.00e-207 8 558 31 587
Crystalstructure of human glycogen branching enzyme (GBE1) [Homo sapiens],4BZY_B Crystal structure of human glycogen branching enzyme (GBE1) [Homo sapiens],4BZY_C Crystal structure of human glycogen branching enzyme (GBE1) [Homo sapiens]
5CLT_A 6.58e-203 15 558 6 555
Crystalstructure of human glycogen branching enzyme (GBE1) in complex with acarbose [Homo sapiens],5CLT_B Crystal structure of human glycogen branching enzyme (GBE1) in complex with acarbose [Homo sapiens],5CLT_C Crystal structure of human glycogen branching enzyme (GBE1) in complex with acarbose [Homo sapiens],5CLW_A Crystal structure of human glycogen branching enzyme (GBE1) in complex with maltoheptaose [Homo sapiens],5CLW_B Crystal structure of human glycogen branching enzyme (GBE1) in complex with maltoheptaose [Homo sapiens],5CLW_C Crystal structure of human glycogen branching enzyme (GBE1) in complex with maltoheptaose [Homo sapiens]
3AMK_A 5.81e-191 4 575 9 591
Structureof the Starch Branching Enzyme I (BEI) from Oryza sativa L [Oryza sativa Japonica Group]
3VU2_A 1.65e-190 4 575 9 591
Structureof the Starch Branching Enzyme I (BEI) complexed with maltopentaose from Oryza sativa L [Oryza sativa Japonica Group],3VU2_B Structure of the Starch Branching Enzyme I (BEI) complexed with maltopentaose from Oryza sativa L [Oryza sativa Japonica Group]
7ML5_A 2.25e-190 4 575 8 590
ChainA, Isoform 2 of 1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic [Oryza sativa Japonica Group]

Swiss-Prot Hits      download full data without filtering help

Created with Snap3366991321661992322652983323653984314644985315645976302558sp|Q555Q9|GLGB_DICDI8558sp|Q6EAS5|GLGB_HORSE1572sp|Q8NKE1|GLGB_RHIID4558sp|Q96VA4|GLGB_ASPOR3558sp|Q9Y8H3|GLGB_EMENI
Hit ID E-Value Query Start Query End Hit Start Hit End Description
Q555Q9 4.36e-211 2 558 7 566
1,4-alpha-glucan-branching enzyme OS=Dictyostelium discoideum OX=44689 GN=glgB PE=3 SV=1
Q6EAS5 7.08e-210 8 558 28 584
1,4-alpha-glucan-branching enzyme OS=Equus caballus OX=9796 GN=GBE1 PE=1 SV=1
Q8NKE1 1.35e-208 1 572 7 586
1,4-alpha-glucan-branching enzyme OS=Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) OX=747089 GN=GLC3 PE=2 SV=2
Q96VA4 1.32e-207 4 558 13 575
1,4-alpha-glucan-branching enzyme OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=gbeA PE=2 SV=1
Q9Y8H3 3.18e-207 3 558 7 570
1,4-alpha-glucan-branching enzyme OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=be1 PE=2 SV=3

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000057 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000000121_00810.