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CAZyme Information: MGYG000001603_00144

You are here: Home > Sequence: MGYG000001603_00144

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species UMGS1370 sp900551135
Lineage Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; UMGS1370; UMGS1370 sp900551135
CAZyme ID MGYG000001603_00144
CAZy Family CBM48
CAZyme Description 1,4-alpha-glucan branching enzyme GlgB
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
636 73652.46 6.6892
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000001603 2462371 MAG China Asia
Gene Location Start: 5634;  End: 7544  Strand: -

Full Sequence      Download help

MPRKTKKTSG  TGFITEMDRY  LFGQGTHYEI  YEKLGAHPKT  YKKQDGMYFA  VWAPHAQQVS60
LVGDFNGWNP  DSNPMTVLED  SGIWEIFIPG  MTTGQLYKYA  ITTHTGKILF  KADPYAFSAE120
YRPGTASVTA  DINDFKWTDE  KWMNLRTSTV  PEQAPVSIYE  VHLGSWRKKY  RPEKDGYYTY180
KEAAHELAAY  VKEMGYTHVE  LMGIAEHPFD  GSWGYQVTGY  FAPTSRYGLP  KDFMYFVNYL240
HKQGIGVILD  WVPAHFPKDA  HGLADFDGQA  LYEYADPRKG  EHPDWGTKVF  DYGKNEVKNF300
LIANAIYWLE  KFHVDGLRVD  AVASMLYLDY  GRKDGEWIPN  RYGGNQNLEA  IEFFKHLNSV360
LSGRKNGAMV  IAEESTAWPN  VTRRPEDDGL  GFTFKWNMGW  MHDFLEYMKL  DPYFRKYNHN420
KMTFGLTYFT  SENFILVLSH  DEVVHLKCSM  INKMPGLYDD  KFANLKAGYT  FMMGHPGKKL480
LFMGQEFGQW  HEWDEKVSLD  WQLLDEDKHK  ELQSYVKDLL  HLYRKYPALY  KLDNDWNGFQ540
WINANDGDRS  IFSFVRRDET  GKKNLLFIIN  FTPVPRPDYC  IGVPKKGKYT  LILDNEHGML600
PASDAPSFKS  SAKECDGQPY  SFDYPLPAYG  TAVFRF636

Enzyme Prediction      help

EC 2.4.1.18

CAZyme Signature Domains help

Created with Snap316395127159190222254286318349381413445477508540572604190489GH1333120CBM48
Family Start End Evalue family coverage
GH13 190 489 1.3e-155 0.9933554817275747
CBM48 33 120 1.3e-21 0.9210526315789473

CDD Domains      download full data without filtering help

Created with Snap31639512715919022225428631834938141344547750854057260414635PRK0540214634PRK1470512636PRK12313122525AmyAc_Glg_BE14635GlgB
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
PRK05402 PRK05402 0.0 14 635 100 722
1,4-alpha-glucan branching protein GlgB.
PRK14705 PRK14705 0.0 14 634 603 1220
glycogen branching enzyme; Provisional
PRK12313 PRK12313 0.0 12 636 5 627
1,4-alpha-glucan branching protein GlgB.
cd11322 AmyAc_Glg_BE 0.0 122 525 2 402
Alpha amylase catalytic domain found in the Glycogen branching enzyme (also called 1,4-alpha-glucan branching enzyme). The glycogen branching enzyme catalyzes the third step of glycogen biosynthesis by the cleavage of an alpha-(1,4)-glucosidic linkage and the formation a new alpha-(1,6)-branch by subsequent transfer of cleaved oligosaccharide. They are part of a group called branching enzymes which catalyze the formation of alpha-1,6 branch points in either glycogen or starch. This group includes proteins from bacteria, eukaryotes, and archaea. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
COG0296 GlgB 0.0 14 635 6 627
1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].

CAZyme Hits      help

Created with Snap3163951271591902222542863183493814134454775085405726046636QIX92210.1|CBM48|GH13_96636QRP41890.1|CBM48|GH13_96636ASN93451.1|CBM48|GH13_96636QJU22196.1|CBM48|GH13_96636ANU46944.1|CBM48|GH13_9
Hit ID E-Value Query Start Query End Hit Start Hit End
QIX92210.1 0.0 6 636 13 642
QRP41890.1 0.0 6 636 13 642
ASN93451.1 0.0 6 636 13 642
QJU22196.1 0.0 6 636 13 642
ANU46944.1 0.0 6 636 13 642

PDB Hits      download full data without filtering help

Created with Snap316395127159190222254286318349381413445477508540572604136355GQW_A136355GR5_A136355GQZ_A136355GR2_A136355GQU_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
5GQW_A 9.60e-237 13 635 128 772
Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A 1.36e-236 13 635 128 772
Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A 1.92e-236 13 635 128 772
Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR2_A 1.92e-236 13 635 128 772
Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQU_A 2.73e-236 13 635 128 772
Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits      download full data without filtering help

Created with Snap31639512715919022225428631834938141344547750854057260413636sp|P30539|GLGB_BUTFI14635sp|Q7NL20|GLGB_GLOVI14596sp|B3PGN4|GLGB_CELJU14635sp|Q2JT08|GLGB_SYNJA14635sp|Q2JK68|GLGB_SYNJB
Hit ID E-Value Query Start Query End Hit Start Hit End Description
P30539 2.73e-299 13 636 6 632
1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q7NL20 1.45e-246 14 635 103 727
1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
B3PGN4 4.72e-246 14 596 115 693
1,4-alpha-glucan branching enzyme GlgB OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=glgB PE=3 SV=1
Q2JT08 1.44e-245 14 635 117 752
1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=glgB PE=3 SV=1
Q2JK68 3.01e-243 14 635 117 752
1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000053 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000001603_00144.