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CAZyme Information: MGYG000003700_00184

You are here: Home > Sequence: MGYG000003700_00184

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Collinsella sp900546115
Lineage Bacteria; Actinobacteriota; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Collinsella; Collinsella sp900546115
CAZyme ID MGYG000003700_00184
CAZy Family CBM48
CAZyme Description 1,4-alpha-glucan branching enzyme GlgB
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
753 85039.69 6.0491
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000003700 2274583 Isolate China Asia
Gene Location Start: 211601;  End: 213862  Strand: -

Full Sequence      Download help

MSDTKLKPAT  KKPATRKAAP  HAPELSKDDV  YLFGIGTWER  SWEKMGAHPD  TQNRTRGWRF60
CVWAPDVKSV  HVIGEFNDWD  EEANPLVPVH  TSAIWEGFIP  GAEQGQLYKY  LIETNEGEKL120
YKADPYAFKA  ECPPGTASVL  WTLDGYKWND  AAWLKRRASH  NHMSQPLNIY  EVHIGSWKRH180
GDAPQGEPDE  YGNYPGPMDP  FPAQRGEFYT  YDDLSVELVD  YVHDMGYTHI  EVMPLMEHPF240
DGSWGYQTTG  YYAATSRYGD  PQQLMHFIDA  CHEAGIGVIM  DWVPGGFCAD  SHGLATFNGH300
MLFEHEIHPN  WGTHKFDFAR  GEVRSFLVSN  VLYWLENFHV  DGIRMDGVSS  MLYLNFGIDD360
PGQKKFNKYG  TEEDLDASAF  IRQVNCAVEA  HFPDVMMIAE  ESTAWPLVTY  PPQDGGLGFH420
YKWDMGWMND  TLHYMQTDFP  WRPGNHGLLT  FSIMYAFTEN  FICPLSHDEV  VHGKCSLIGR480
MPGDWWRQFA  GLRTLAFYQM  THPGAKLNFM  GNEIGQFIEW  RYYESIQWFL  TEEYETHRHH540
QAFIKALNHL  YTAEPALYER  GYTDDGFTWI  DADNSKQSIV  SFVRQGEDVD  DDLVILINFD600
PASYESFRVG  VPREGDWEVI  FDSDRPEFGG  SGYAGEEPYT  CSSEPYPWNG  QMDSIEIKVP660
GLAGVVLKRR  GPSSYKPPVV  EEPKAAPKKR  MSSVKPKPAA  AKKAPAKAKA  TTTKAKAKAA720
AKPAAKAAAK  KPAAKKAATK  AKSASVKPSA  KDA753

Enzyme Prediction      help

EC 2.4.1.18

CAZyme Signature Domains help

Created with Snap3775112150188225263301338376414451489527564602640677715220516GH1344130CBM48
Family Start End Evalue family coverage
GH13 220 516 1.5e-136 0.9966777408637874
CBM48 44 130 5.6e-19 0.9078947368421053

CDD Domains      download full data without filtering help

Created with Snap377511215018822526330133837641445148952756460264067771523671PRK1231323669GlgB23662PRK14705132551AmyAc_Glg_BE21671PRK05402
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
PRK12313 PRK12313 0.0 23 671 5 629
1,4-alpha-glucan branching protein GlgB.
COG0296 GlgB 0.0 23 669 4 628
1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14705 PRK14705 0.0 23 662 601 1215
glycogen branching enzyme; Provisional
cd11322 AmyAc_Glg_BE 0.0 132 551 1 400
Alpha amylase catalytic domain found in the Glycogen branching enzyme (also called 1,4-alpha-glucan branching enzyme). The glycogen branching enzyme catalyzes the third step of glycogen biosynthesis by the cleavage of an alpha-(1,4)-glucosidic linkage and the formation a new alpha-(1,6)-branch by subsequent transfer of cleaved oligosaccharide. They are part of a group called branching enzymes which catalyze the formation of alpha-1,6 branch points in either glycogen or starch. This group includes proteins from bacteria, eukaryotes, and archaea. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
PRK05402 PRK05402 0.0 21 671 96 725
1,4-alpha-glucan branching protein GlgB.

CAZyme Hits      help

Created with Snap37751121501882252633013383764144514895275646026406777151753AZH69616.1|CBM48|GH13_91690ATP53564.1|CBM48|GH13_91703QIA34706.1|CBM48|GH13_925673AEB07177.1|CBM48|GH13_922703BBH49561.1|CBM48|GH13_9
Hit ID E-Value Query Start Query End Hit Start Hit End
AZH69616.1 0.0 1 753 6 758
ATP53564.1 0.0 1 690 6 695
QIA34706.1 0.0 1 703 5 707
AEB07177.1 0.0 25 673 28 674
BBH49561.1 0.0 22 703 14 695

PDB Hits      download full data without filtering help

Created with Snap3775112150188225263301338376414451489527564602640677715226685GQZ_A226685GQU_A226685GR2_A226685GQW_A226685GR5_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
5GQZ_A 3.43e-204 22 668 126 772
Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A 3.43e-204 22 668 126 772
Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A 9.69e-204 22 668 126 772
Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQW_A 1.37e-203 22 668 126 772
Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A 1.37e-203 22 668 126 772
Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits      download full data without filtering help

Created with Snap377511215018822526330133837641445148952756460264067771517668sp|Q24VW3|GLGB_DESHY29668sp|B8CVY1|GLGB_HALOH22668sp|Q8DLB8|GLGB_THEVB25663sp|Q8CZE8|GLGB_OCEIH23668sp|Q7UVH1|GLGB_RHOBA
Hit ID E-Value Query Start Query End Hit Start Hit End Description
Q24VW3 2.97e-228 17 668 4 628
1,4-alpha-glucan branching enzyme GlgB OS=Desulfitobacterium hafniense (strain Y51) OX=138119 GN=glgB PE=3 SV=1
B8CVY1 1.27e-226 29 668 10 626
1,4-alpha-glucan branching enzyme GlgB OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) OX=373903 GN=glgB PE=3 SV=1
Q8DLB8 3.32e-211 22 668 103 749
1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q8CZE8 2.36e-210 25 663 4 621
1,4-alpha-glucan branching enzyme GlgB OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831) OX=221109 GN=glgB PE=3 SV=1
Q7UVH1 2.27e-209 23 668 107 733
1,4-alpha-glucan branching enzyme GlgB OS=Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1) OX=243090 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000041 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000003700_00184.