CAZyme Information: MGYG000001224_00092

Basic Information

• Species: Blautia_A sp900547615
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia_A; Blautia_A sp900547615
• CAZyme ID: MGYG000001224_00092
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
585	MGYG000001224_1|CGC2	67964.99	5.2844

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001224	2725893	MAG	Austria	Europe

• Gene Location: Start: 96616; End: 98373 Strand: +

Full Sequence      

MYRSEYPRPEFKREEWLSLNGQWDFCFPGEEMRKIEVPFVFQSEMSGIHENRMCDSVTYK
RSFCVPDKWKGKRIRIHFGAVDYACSVFINEKKVGSHQGGNTPFSFDITEELTWKKEEIC
VQVWDPCRDETIPRGKQFWKKDPDSIWYTRSTGIWQSVFIEPVSEVHFSYIRFTPDIDRG
TVEVKFKAAGLKEKIGGCHAEFAIIFKEREVFRGGMELLEDYGRMEISLFDRHIFRTMNH
DGGWCWSPESPNLFDVSAALVKEGETIDQVCTYFGMRKIEQKNGMVYLNNRPYYQKLILD
QGYWPGSLMTAQDEQDFVKDIEMAKAMGFNGCRKHQKAEDPVFLYYADRIGFLVWSEISA
CASYSLEASQRTVTEWGEAIQRDYNHPCIVAWVVLNESWGVPNICFDKRQQAHSLALYYN
AKSMDTTRLVISNDGWEITKTDICAIHNYAHGEQSEPVKQEIFRKTLSTKEDILKAQPAG
RQIYADGFGHQGEPVLLTEFGGLAYAKDREKGWGYTTIENEEEFLEVYGRILKDIADSEI
LFGFCYTQLCDVEQEVNGLLTYDRKYKVAPEKIKAINDSVRKDYL

Enzyme Prediction     

No EC number prediction in MGYG000001224_00092.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	11	452	5.2e-97	0.5106382978723404

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	3.02e-36	1	430	1	429	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	4.70e-30	6	397	3	417	beta-D-glucuronidase; Provisional
PRK10340	ebgA	6.69e-25	25	430	95	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.68e-20	18	430	54	485	beta-galactosidase.
pfam02837	Glyco_hydro_2_N	3.95e-14	18	160	3	165	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AGA57247.1	3.24e-255	3	577	4	589
SQI62769.1	1.26e-254	1	585	1	596
AQU76856.1	3.44e-249	1	578	1	589
QDM46707.1	4.19e-243	2	578	3	590
QNF29847.1	3.80e-241	2	578	3	590

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	9.30e-88	5	580	28	582	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
4JKM_A	1.09e-22	17	397	16	415	CrystalStructure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],4JKM_B Crystal Structure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],6CXS_A Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13],6CXS_B Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13]
6D8K_A	1.84e-21	18	432	37	442	Bacteroidesmultiple species beta-glucuronidase [Bacteroides ovatus],6D8K_B Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_C Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_D Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus]
4YPJ_A	2.04e-21	11	546	18	561	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5UJ6_A	2.07e-20	14	397	32	418	CrystalStructure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],5UJ6_B Crystal Structure of Bacteroides Uniformis beta-glucuronidase [Bacteroides uniformis str. 3978 T3 ii],6NZG_A Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis],6NZG_B Bacteroides uniformis beta-glucuronidase 2 covalently bound to cyclophellitol-6-carboxylate aziridine [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P77989	1.24e-28	15	415	11	406	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KPJ7	2.36e-24	59	403	107	436	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
O52847	8.03e-22	9	430	83	504	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1
P26257	4.24e-21	35	533	40	488	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
Q6D736	1.26e-19	35	457	118	522	Beta-galactosidase OS=Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) OX=218491 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000094	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001224_00092.