CAZyme Information: MGYG000001869_00080

Basic Information

• Species: HGM12619 sp900753195
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; UBA644; HGM12619; HGM12619 sp900753195
• CAZyme ID: MGYG000001869_00080
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
585	MGYG000001869_1|CGC2	67246.28	4.9466

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001869	1750418	MAG	Denmark	Europe

• Gene Location: Start: 88327; End: 90084 Strand: -

Full Sequence      

MDTKIPRSEYPRPQLVRKDWLCLNGEWNFETDNEKSDMTRQLYNTPDLKGKITVPFCPES
RLSGVENTDFMPCVWYSRRLSIPENWTGKRILLHFGAVDYSATVFINGEEVTGHQGGYTP
FYADITEKLNRKNDFVTLCVYDDVRSHKQPGGKQSISRNSNGCFYTRTTGIWQSVWMEAV
DECWIKDISFTPDIDSGSVSVRIKLSGNFPGSSLSLRIFWDGKETGRQNIGNIRTDTLCF
SVPLSEIHLWDVGQGNLYSVSVCVEKQSRVLDEAESYFGMRSICLDGRKFIINGRPVFGR
WVLDQGYYPDGIYTAPTDEDLENDIKYAMALGFNGARLHQKIFDPRYLYHADKLGFLCWG
EHANWSLDVTDPGSILNFLPEWTEAVQRDYSHPSVIGWCPFNETWNTEAGKQCDGVIDTV
YRITKALDPTRPVIDTSGNFHVQSDIFDVHDYEQDPKAFAEYYAHIPDGIIKDQIWRNPN
IRDRQHYNGKDPVFVSEYGGIGWMPGSNSGWGYGNGPATEEEFISRYRDLTDILLDNEYI
MGFCYTQLYDIEQEKNGLMTYDRHFKFPPETIKKINSRKATIEKD

Enzyme Prediction     

No EC number prediction in MGYG000001869_00080.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	16	498	2.2e-98	0.5345744680851063

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	1.46e-42	12	433	3	445	beta-D-glucuronidase; Provisional
COG3250	LacZ	1.08e-39	6	473	1	466	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	3.49e-19	39	433	91	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	3.98e-14	23	433	55	485	beta-galactosidase.
pfam02837	Glyco_hydro_2_N	2.21e-13	23	141	4	134	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIQ16879.1	1.26e-235	5	583	7	581
AIQ73223.1	6.33e-231	5	583	7	581
ANY74929.1	1.04e-229	3	583	5	581
QOS82722.1	9.04e-228	5	583	4	579
AWV32566.1	9.69e-228	5	583	7	581

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	9.98e-81	9	578	28	581	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
3K4A_A	2.80e-32	23	499	17	508	Crystalstructure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12],3K4A_B Crystal structure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12]
4JHZ_A	5.00e-32	23	499	17	508	Structureof E. coli beta-Glucuronidase bound with a novel, potent inhibitor 2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-N-[(1S,2S,5S)-2,5-dimethoxycyclohexyl]acetamide [Escherichia coli K-12],4JHZ_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-N-[(1S,2S,5S)-2,5-dimethoxycyclohexyl]acetamide [Escherichia coli K-12]
3LPF_A	5.07e-32	23	499	17	508	Structureof E. coli beta-Glucuronidase bound with a novel, potent inhibitor 1-((6,7-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(3-methoxyphenyl)thiourea [Escherichia coli K-12],3LPF_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 1-((6,7-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(3-methoxyphenyl)thiourea [Escherichia coli K-12],3LPG_A Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 3-(2-fluorophenyl)-1-(2-hydroxyethyl)-1-((6-methyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)urea [Escherichia coli K-12],3LPG_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 3-(2-fluorophenyl)-1-(2-hydroxyethyl)-1-((6-methyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)urea [Escherichia coli K-12],5CZK_A Structure of E. coli beta-glucuronidase bound with a novel, potent inhibitor 1-((6,8-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(4-hydroxyphenyl)thiourea [Escherichia coli K-12],5CZK_B Structure of E. coli beta-glucuronidase bound with a novel, potent inhibitor 1-((6,8-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(4-hydroxyphenyl)thiourea [Escherichia coli K-12]
6LEM_B	1.62e-31	23	499	13	504	ChainB, Beta-D-glucuronidase [Escherichia coli]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P05804	8.96e-31	23	499	15	506	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
O18835	7.18e-28	10	499	29	541	Beta-glucuronidase OS=Canis lupus familiaris OX=9615 GN=GUSB PE=1 SV=1
Q4FAT7	7.21e-28	23	499	42	542	Beta-glucuronidase OS=Sus scrofa OX=9823 GN=GUSB PE=3 SV=1
O97524	1.71e-27	10	499	29	541	Beta-glucuronidase OS=Felis catus OX=9685 GN=GUSB PE=1 SV=1
P06760	7.19e-27	18	433	36	475	Beta-glucuronidase OS=Rattus norvegicus OX=10116 GN=Gusb PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000048	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001869_00080.